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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Recombinant Mouse Fgf18 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: Recombinant human insulin (hINS) powder (Sigma Aldrich) was dissolved in 20 mM HCl (pH 2) ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant human TNFα (30 ng/mL, Sigma), and C1q (400 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant Human Insulin (Sigma cat no. I9278) 0.02mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant plasmids were transformed into TUNER (Novagen) cells in LB broth containing 10 μg/ml kanamycin at 37 °C shaking at 180 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... 0.002 mg/mL recombinant human insulin (Sigma), 0.1% Trace Elements A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... DNAse I recombinant (Roche by Sigma Aldrich) and sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10nM recombinant human (Leu15)-gastrin I (Sigma), 50ng/ml recombinant human EGF (Peprotech) ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant 6His-ErbB3 was purchased from Sigma. The in vitro binding assay was conducted by incubating 1 μg/ml recombinant ErbB3 with 1 μg/ml recombinant Rabaptin5 in a stringent buffer containing 50 mM Tris ...
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bioRxiv - Bioengineering 2021Quote: ... 0.002 mg/ml recombinant human insulin (Sigma), 0.1% Trace Elements A (Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... 0.5% recombinant human serum albumin (A9986, Sigma), 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... a recombinant HRV 3C protease (Sigma-Aldrich) was added to NSP7/NSP8 complex and incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant 2N4R human Tau (Tau441, AG960, Millipore) at serial dilutions ranging from 20 µg/ml to 0 µg/ml total tau was used to generate a calibration curve and calculate total tau in our samples.
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bioRxiv - Cell Biology 2022Quote: ... 0.025-μg·ml-1 recombinant human EGF (Sigma), 0.1-μg·ml-1 cholera toxin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... 10 μg/ml recombinant human insulin (Sigma), 2 IU/ml heparin (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL human recombinant bFGF (Sigma), and 10 μM Y-27632 (STEMCELL Technologies)) ...
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bioRxiv - Genomics 2023Quote: ... 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Microbiology 2024Quote: ... 5 ng of recombinant human AChE (Sigma) or 30 μg of tissue lysate was incubated with steroidal alkaloid compound or at room temperature for 30 minutes in 50 mM Tris pH 8.0 in a total volume of 90 μL ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant human IFN-α2a (H6041; Sigma-Aldrich) was reconstituted in PBS with 0.1% BSA and added to the basolateral pole at a final concentration of 100 U/ml ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.