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Citations for Millipore Sigma :
351 - 400 of 1038 citations for Rcombinant Cynomolgus PDCD1LG2 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal calf serum (FCS; Sigma-Aldrich or Gibco), 2 mM Glutamine (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... FCs were incubated with 1% (v/v) casein solution (Sigma-Aldrich) for 3 to 4 h and flushed with 1 ml buffer (20 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FCS) (Sigma-Aldrich). Unless specified otherwise ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T were grown in DMEM supplemented with 10% FCS (Sigma-Aldrich). HUVEC were cultured in Endothelial Cell Growth Medium (Cell Applications ...
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bioRxiv - Biochemistry 2022Quote: ... the secondary antibody HRP anti-Human IgG (Fc specific) (Sigma-Aldrich) was added (1:15,000) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were imaged in DMEM supplemented with 10% FCS (Sigma-Aldrich), 100U/ml penicillin ...
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bioRxiv - Immunology 2020Quote: ... in RPMI supplemented with 10% foetal calf serum (FCS) (Sigma-Aldrich), 100 U/mL penicillin ...
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bioRxiv - Microbiology 2020Quote: ... streptomycin (100 μg/ml) and 10% fetal calf serum (FCS) (Sigma). The recombinant VACV VT7LacOI ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% heat-inactivated FCS and 1 % Penicillin/Streptomycin (Sigma cat#P0781) in a 28°C incubator with 5 % CO2 to 80-90 % confluency before harvesting ...
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bioRxiv - Neuroscience 2021Quote: ... eyes were incubated in 20% foetal bovine serum (FCS) (F7524; Sigma) in order to block proteinase K activity ...
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bioRxiv - Biochemistry 2020Quote: ... goat-anti-mIgG(Fc) conjugated with HRP (1:42000, A0168, Sigma) for mFc tagged antigen versions or goat-anti-hIgG(Fc ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorinated oil (Fluorinert FC-40, Cat# 86508-42-1, Sigma-Aldrich) was loaded into the device for sealing microwell arrays after introducing freeze-thaw lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1% FCS and with 0.1% Penicillin Streptomycin (PS, Sigma), minced and incubated at 37°C with the digestion solution (collagenase 2.5mg/ml in EBSS) ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-human Fc antibody (1:10000 dilution, Sigma Aldrich) was used ...
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bioRxiv - Microbiology 2020Quote: ... streptomycin (100 mg/ml) and 5% fetal calf serum (FCS) (Sigma). IBDV infections were performed on preconfluent (ca ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 2% (v/v) FCS and 2mM EDTA (Sigma, E7889). Wash buffer consists of PBS supplemented with 2% FCS.
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Biophysics 2022Quote: ... containing 10mM HEPES (Roth) and 0.5% FCS (Biochrom GmbH, Merck Millipore). One chamber slide was used for gel expansion and the other one as non-expanded control ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C at any time ...
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bioRxiv - Bioengineering 2023Quote: ... 100μL of ALP-conjugated anti-human IgG Fc-ALP (Sigma-Aldrich) diluted 1:5000 in M-PBST was added and incubating for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Media were supplemented with 5% fetal calf serum (FCS) (Sigma-Aldrich) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with RPMI containing 10% (v/v) FCS (Sigma) and 2 mM L-Glutamine (R10) ...
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bioRxiv - Immunology 2023Quote: ... and 10% (v/v) heat-inactivated fetal calf serum (FCS) (Sigma). Cells were added to sterile 96 well U-bottom tissue culture plates pre-coated with purified NA/LE anti-CD3 (Clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich #A2429), at a 1:4000 dilution was added ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µM ABA or 1 µM Fusicoccin (FC) (all from Sigma) dissolved in absolute ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 20% fetal calf serum (FCS; Sigma-Aldrich cat# F7524) and 50 ng/mL vascular endothelial growth factor (VEGF165 ...
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bioRxiv - Cell Biology 2024Quote: ... was used to prepare unstimulated cells or FCS (Biosera or Sigma) to prepare stimulated cells ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral particles encoding Flag-tagged human PDHA1 or shRNAs targeting mouse Pdha1 (TRCN0000041914/TRCN0000041914 (Sigma)) were produced in 293T packaging cells by transient transfection using Jet-PEI reagent (Ozyme) ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged Scube was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...