Labshake search
Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
-
bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody OC (1:500 for mouse, 1:200 for human; polyclonal, AB2286, Millipore) and respective secondary antibody (1:400 ...
-
bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
-
bioRxiv - Bioengineering 2019Quote: ... Both fibroblast cell types were subsequently growth arrested by incubating with 1 μg/mL mitomycin-C (Sigma- Aldrich, St. Louis, MO) in culture medium for 4 hours prior to detachment from the culture substrates using trypsin as previously described 7 ...
-
bioRxiv - Genetics 2021Quote: ... Collected blastocysts were cultured on mitomycin-treated mouse embryonic fibroblast feeder cells in 2i-LIF medium (ESGRO Complete Basal medium (Millipore, Germany) supplemented with leukemia inhibitory factor (Wako ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were maintained on a layer of 40k per cm2 of mouse embryonic fibroblasts mitotically inactivated by Mitomycin C (Sigma M4287). Cultures were kept in incubators with 5% CO2 at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM, Sigma) in PBS with 1x cOmplete™ protease inhibitor cocktail (Roche Diagnostics ...
-
bioRxiv - Biophysics 2023Quote: 3T3 fibroblast and MV3 cells were cultured in high-glucose Dulbecco Modified Eagle’s Medium (D6546; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal calf serum (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2021Quote: AC16 cells (a proliferating cell line derived from human cardiomyoctyes16) were purchased from Millipore. Stable GCN5L1 knockdown was generated as previously described.17 Cells were treated with 10 nM 17β-estradiol (E2) ...
-
bioRxiv - Immunology 2023Quote: The BLaER1 Human B-cell Precursor Leukemia Cell Line was purchased from Millipore Sigma− Aldrich and cultured as described53 ...
-
bioRxiv - Developmental Biology 2024Quote: Human TS cells or differentiated EVT cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 20 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: HeLa (human cervix epithelial cells) cells were cultured in Minimum Essential Medium (MEM, Sigma) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2020Quote: Cells were immmunolabeled with primary antibodies targeting actin (#A2066; Sigma-Aldrich), Arl13b (#17711-1-AP ...
-
bioRxiv - Neuroscience 2021Quote: Primary microglia and BV2 cells were lysed in RIPA buffer (Sigma) containing protease and phosphatase inhibitor cocktail (Fisher) ...
-
bioRxiv - Genetics 2019Quote: ... Yeast cells were stained with primary antibody (anti-HA, Sigma-Aldrich, ref ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with primary antibody against SON (Sigma, HPA 023535) at a 1:300 dilution in DEPC-treated PBS for 1 hr in a humid chamber at RT ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were incubated with primary antibodies against γ-tubulin (Sigma, GTU88) and phospho-histone H3 serine 10 (PHH3 ...
-
bioRxiv - Immunology 2020Quote: ... Cells were incubated with primary anti-vinculin antibody (Sigma 1:200) for 1 hour at room temperature ...
-
bioRxiv - Biophysics 2020Quote: ... Cells were then incubated with primary antibodies to vinculin (Sigma V9131) or paxillin (Abcam 32084 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with unconjugated primary antibody: LAMC2 (SIGMA, HPA024638-100U) overnight at 4°C in the dark ...
-
bioRxiv - Neuroscience 2021Quote: ... Target cells were stained with α-TH primary antibody (Millipore, AB152) as in.[73] Imaging was performed using the confocal laser scanning microscope Leica TCS SP8 ...
-
bioRxiv - Genomics 2019Quote: ... Cells were then incubated with primary antibodies (1:400 MAP2 (Sigma) and 1:2000 BAF170 (Active Motif) ...
-
bioRxiv - Immunology 2021Quote: ... primary macrophages (1×104 cells) on poly-L-Lysine (Sigma, USA) coated glass cover slips were rinsed with PBS ...
-
bioRxiv - Cancer Biology 2020Quote: Primary tumor cells were lysed in cold RIPA buffer (Sigma Aldrich) supplemented with Aprotinin (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary acetylated tubulin mouse antibody (Sigma Aldrich) diluted 1:200 in 1% BSA in PBS and incubated for 1.5 hour at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T cells pre-stimulated with PHA (Sigma, 1µg/ml) and 10 ng/ml IL-2 (Stemcelll ...
-
bioRxiv - Immunology 2022Quote: A20 cell lines and primary B cells were seeded onto poly-L-lysine (Sigma-Aldrich) coated coverslips ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... Cardiac perfusion was performed with PBS followed immediately by ice-cold 4% PFA prepared in PB buffer (Sigma-Aldrich). Brains were removed and further fixed in 4% PFA for 24 hours at 4°C to ensure complete fixation ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
-
bioRxiv - Genetics 2022Quote: ... human HEK 293T embryonic kidney cells (Intercell, AG) and mouse MEFs embryonic fibroblasts Pml−/− (from Dr. Lallemand-Breitenbach) were cultivated in DMEM medium (Sigma-Aldrich, D6429) containing 10% of fetal calf serum (FCS ...
-
bioRxiv - Developmental Biology 2023Quote: A feeder layer of inactivated murine embryonic fibroblast cells was cultured in 6-well plates on gelatin (0.1%, Sigma-Aldrich G7041-100G) at a seeding density of 1.6 x 104 cells/cm2 in base medium consisting of DMEM (1x ...
-
bioRxiv - Developmental Biology 2023Quote: ... a feeder layer of inactive murine embryonic fibroblast cells was cultured in 6-well plates coated with 0.1% gelatin (Sigma-Aldrich G7041-100G) at a density of 1.6 x 104 cells/cm2 in a base medium containing DMEM (1x ...
-
bioRxiv - Genomics 2020Quote: ... Mouse Embryonic fibroblasts were crosslinked using 1% formaldehyde (Sigma cat. No. F8775) for 10 minutes followed by quenching with 0.125 M Glycine for 5 minutes and lysis with lysis buffer 1 -LB1- (50 mM Hepes KOH pH 7.5 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Blastocysts were cultured on irradiated mouse embryo fibroblasts (PMEF-N, Millipore Sigma) in ESGRO-2i medium (Millipore Sigma SF016-100 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
-
bioRxiv - Microbiology 2020Quote: Murine L929 fibroblasts were maintained in Joklik’s modified Eagle medium (JMEM, Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Systems Biology 2020Quote: ... and basic fibroblast growth factor (10 ng/mL; Merck Millipore, Watford, UK). Decidualization experiments were carried out at passage 2 ...
-
bioRxiv - Neuroscience 2019Quote: ... and basic fibroblast growth factor (25 μg/ml, Sigma-Aldrich, MO, USA). Differentiation was induced after 5 days in vitro (DIV ...
-
bioRxiv - Genetics 2020Quote: ... 10 ng/μL recombinant fibroblast growth factor basic (βFGF; Sigma-Aldrich, #F0291), 10 μg/μL Heparin (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... mouse embryonic fibroblasts were treated with Brefeldin A (B7651-5MG, Sigma Aldrich). qRT-PCR was performed using Fast SYBR Green master mix (Applied Biosystems ...
-
bioRxiv - Developmental Biology 2021Quote: ... Chick embryonic fibroblasts (CEF) were cultured in F-12/HAM (Sigma, #N6658) with 10% FCS ...
-
bioRxiv - Genomics 2020Quote: ... 4 ng/ml bovine Fibroblast Growth Factor and Insulin – Transferrin Selenium (Sigma).
-
bioRxiv - Immunology 2022Quote: ... fibroblasts were treated with BACE1 inhibitors (M-3 (500nM-4μM, Sigma-Aldrich) or AZD3839 (500nM-2μM ...
-
bioRxiv - Bioengineering 2023Quote: ... 1 ng/mL basic fibroblast growth factor (bFGF; Sigma-Aldrich, cat# F0291), 100 μg/mL heparin (Sigma-Aldrich ...
-
bioRxiv - Genetics 2023Quote: ... and 10 ng/mL basic fibroblast growth factor (EMD/Millipore, GF003AF-MG). The medium was replaced every 2 days ...
-
bioRxiv - Bioengineering 2019Quote: Genotoxicity assay was determined using Hek 293T cells (Human embryonal kidney cells, Sigma-Aldrich, USA) according to Comet assay as previously described [31] ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then stained with anti-human-IgG-FITC (Sigma, F9512) or anti-mouse-IgG-FITC (Sigma ...
-
bioRxiv - Immunology 2022Quote: Skin cells were incubated with 50 µg/ml human IgG (Sigma), Near IR Dead cell stain (Invitrogen ...