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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for PD 1 Human C93S HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cryosections of PD-fed mice were used for picrosirius red staining (Sigma–Aldrich, Germany) according to the manufacturer’s instructions ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... 30 min with MEK inhibitor PD 98059 (50 µM, Cat. no. 513001, Sigma-Aldrich), 30 min with the PKC activator Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a PD-10 desalting column (Sigma-Aldrich, Taufkirchen, Germany), transferred to a new tube ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were collected on Cu/Pd slot grids (Agar Scientific, G2564PD) coated with a film of 1% formvar (Agar Scientific) in chloroform (Sigma Aldrich, 32211-1L-M). To allow better alignment of dual-axis tomography ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse anti human Nestin (Millipore, product no: MAB5326, (1:200)) ...
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bioRxiv - Biochemistry 2021Quote: ... and CF-640R conjugated goat anti-human (1:1000; Sigma-Aldrich) secondary antibodies were used to probe for phospho-Histone H2A.X and β-Actin respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by incubation with rabbit anti-human TCF21 (Sigma, 1:100), mouse-anti-human-CD49e (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... Human head and neck carcinoma line UM-SCC-1 (EMD Millipore) was cultured in DMEM containing 20% FBS ...
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bioRxiv - Genomics 2021Quote: ... human Cot-1 placental DNA from Sigma-Aldrich (Oakville, ON, Canada), and target-specific biotin-labeled probes in hybridization solution ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-human Fc antibody (1:10000 dilution, Sigma Aldrich) was used ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000 HRP-labelled rabbit anti-human IgG (Sigma-Aldrich, #A8792) in blocking solution was added ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were labeled with goat anti-human ChAT (1/500; Millipore) or goat anti-IBA1 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... 1:3000 dilution of anti-human IgA (α-chain-specific) (Sigma) or 1:3000 dilution of anti-human IgM (μ-chain-specific ...
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bioRxiv - Genomics 2021Quote: ... Monoclonal mouse anti-human TRA-1-60 Antibody (Millipore MAB4360C3,1:100), Monoclonal mouse Anti-Stage-Specific Embryonic Antigen-4 Antibody (Millipore ...