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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... followed 46–48 h later by 5 IU human chorionic gonadotropin (Sigma Aldrich, St. Louis, MO). The superovulated females were also bred overnight to vasectomized males for consistency across both treatment groups ...
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bioRxiv - Cell Biology 2023Quote: ... and the oocytes were matured in vivo with 5 U of human chorionic gonadotropin (hCG; Sigma) before mating with B6D2F1/J males (F1 hybrid of a cross between C57BL6/J and DBA2/J ...
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bioRxiv - Cell Biology 2023Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFB1 signaling was induced with 5 ng/ml final concentration of human recombinant TGFB1 (Sigma-Aldrich) for 4 h.
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells were treated with tetracycline hydrochloride (1 µg/ml; T7660, Sigma-Aldrich) for 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... following intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (∼ 90 hours pre-collect) and 5 IU human chorionic gonadotropin (hCG; Sigma; ∼40-48 hours pre-collect) and mating with BDF1 male mice (Jackson 100006) ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... hiPSCs were plated at a density of 4-5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... mice were further injected with 5 IU (0.1 mL/animal) human chorionic gonadotropin (hCG) (Sigma-Aldrich, USA). Twenty hours after PMSG injection ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG; Millipore Sigma, St. Louis, MO). The mice were euthanized 8 hours after hCG treatment and ovaries harvested.
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bioRxiv - Immunology 2021Quote: Lentivirus based knockdown of human ATG16L1 (5’-ACTGTAGCTTTGCCGTGAATG-3’) was performed using MISSION shRNA constructs (Sigma-Aldrich) and psPAX2 packaging system ...
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bioRxiv - Genomics 2020Quote: ... the cells were spun down and moved to “Phase 2” IMDM media containing 5% human serum (Sigma), 330µg/mL transferrin ...
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bioRxiv - Cancer Biology 2022Quote: PSCs were treated in Matrigel in 5%FBS DMEM with 20 ng/mL human TGFβ1 (T7039; Sigma), 300 nM Neratinib (S2150 ...
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bioRxiv - Cancer Biology 2022Quote: PSCs were treated in Matrigel in 5%FBS DMEM with 20 ng/mL human TGFβ1 (T7039; Sigma) for 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich, US), rabbit anti-human Oct4 (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... human Ndc80/Hec1 siRNA (sequence antisense: 5’-GAAUUGCAGCAGACUAUUA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), human KIF4A siRNA (sc-60888 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
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bioRxiv - Immunology 2022Quote: ... and 10U/mL IL-2 (Sigma) in complete RPMI ...
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bioRxiv - Microbiology 2023Quote: ... or IL-1RA (SRP3327, Sigma-Aldrich). At the indicated time points ...
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bioRxiv - Neuroscience 2023Quote: ... Lithium chloride (LiCl; Sigma-Aldrich, IL) was dissolved in water to a concentration of 0.15M and administered intraperitoneally (10 mL/kg).
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bioRxiv - Cell Biology 2023Quote: ... mouse IL-6 (Sigma-Aldrich, RAB0308), mouse TNF-α (Cayman ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... IL-1α (3 ng/ml; Sigma), and C1q (400 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and IL-2 (Millipore, Burlington, MA). Differences in induction of proteins post stimulation were calculated using log10(pg/mL fold-change +1) ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... and Hek293 (ATCC CRL-1573, embryonic kidney) were cultured in DMEM high glucose (Sigma-Aldrich), all supplemented with 2mM glutamine (Biochrome) ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293-GNTI cells were transfected with 1 mg DNA and 3 mg PEI (Sigma-Aldrich) per liter of cells at a density of 1.3-1.8E6 cells/ml ...
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bioRxiv - Cell Biology 2022Quote: SLC1A2/A3-expressing HEK293 Flip-In cells were plated on Poly-L-lysine hydrobromide (Sigma)-coated glass coverslips and induced with doxycycline (1 μg/ml ...