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Citations for Millipore Sigma :
351 - 400 of 3527 citations for FGF 19 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... plasma from COVID-19 patients was loaded into wells of an agarose gel that contained DNA from salmon testes (D1626, Sigma) and SYBR Safe (S33102 ...
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bioRxiv - Microbiology 2020Quote: ... or 40 µg/ml of trimethoprim-sulfamethoxazole (SXT) at a ratio of 1:19 (2 µg/ml of trimethoprim and 38 µg/ml of sulfamethoxazole; Sigma) as a positive control ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli K-12 (ATCC 19020) and Escherichia coli EK-19 were inoculated in sterile Luria Broth (LB, L3522, Sigma) and incubated at 37 °C overnight with a shaking speed of 200 rpm ...
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bioRxiv - Bioengineering 2022Quote: ... samples were blocked and permeabilized with 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma-Aldrich now Merck) in PBS for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... and end of the gradient (fractions 19-21) were combined and concentrated prior to sequencing using an Amicon Ultra-0.5 30KDa filter (Millipore Sigma) [49] ...
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bioRxiv - Physiology 2022Quote: ... Young (12-16 weeks) and aged (18-19 months) mice receiving the solvent (Phosal 50PG (368315, Lipoid) containing 3.3% ethanol (32221, Sigma-Aldrich) and 10% polyethylene glycol 400 (807485 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... fish were kept in the dark with a final concentration of 100 µM yohimbine (CAS: 65-19-0, Sigma-Aldrich) for 30 minutes before imaging as described in30 ...
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bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM total lipid mixtures were extruded through a 0.05 μm pore size 19 mm polycarbonate membrane (Sigma, cat#WHA800308) with filter supports (Avanti ...
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bioRxiv - Biophysics 2023Quote: ... The samples were blocked and simultaneously permeabilized using 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma Aldrich), in PBS (room temperature ...
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bioRxiv - Immunology 2024Quote: ... PMA was prepared based on our previous study (19) in which 1 mg of PMA was dissolved in 1.9 ml of 20% DMSO (Sigma-Aldrich) to obtain a 1 mM stock solution ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2020Quote: Geosmin (CAS #16423-19-1) was used at a concentration of 1:1000 (Sigma-Aldrich, St. Louis, USA, Product Number UC18). 1-Methylindole (CAS #603-76-9 ...
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bioRxiv - Neuroscience 2020Quote: ... myotube compartments were treated with 19 μM of the AChR competitive antagonist tubocurarine hydrochloride pentahydrate (DTC) (Sigma, Cat N° T2379-100G) 10 min before analysis ...
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bioRxiv - Biophysics 2020Quote: ... imaging was performed in a PBS based imaging buffer based on the dSTORM buffer described by van de Linde and others (19) containing 100 mM MEA (Sigma-Aldrich), 0.6 mg/mL Glucose Oxidase (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... hyposmia was induced by bilateral intranasal injections with 20 μL of 5% (0.17M) zinc sulfate (Sigma-Aldrich, catalog #7446-19-7) in male C57BL/6N mice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated for 30 minutes at 37°C in a humidified incubator in 50 µl of monomer solution containing 19% sodium acrylate (Sigma, 408220), 10% acrylamide ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were incubated in primary antibodies diluted in blocking buffer for 1hr (1:1000 PR8 NP polyclonal, gift from Dr. Adolfo Garcia-Sastre; 1:1000 γ-tubulin DQ-19, #T3195 Sigma; 1:1000 α-tubulin ...
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bioRxiv - Molecular Biology 2019Quote: ... sodium acrylate (A, cat. #408220), 19:1 acrylamide/bis-acrylamide (cat. #A2917), and ammonium persulfate (APS, cat. #A3678) were purchased from Sigma Aldrich. Ultrapure N,N,N’,N’-tetramethylethylenediamine (TEMED ...
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bioRxiv - Neuroscience 2019Quote: ... Analysis by Western blot shows a band at 19 kDA from rat olfactory bulb which is blocked by inhibition with specific OMP immunizing peptide (Sigma datasheet).
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bioRxiv - Cell Biology 2022Quote: ... Conditional degradation of mAID tagged EWSR1 protein in the AUX treated OsTIR7-19 cells was confirmed by Immunocytochemistry and Western blot analysis using anti-FLAG (Sigma, #F7425) and anti-EWSR1 (Santa Cruz ...
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bioRxiv - Immunology 2022Quote: ... hamsters were injected intraperitoneally with either PBS or 150 mg/kg and 100 mg/kg of cyclophosphamide (CAS: 6055-19-2; PHR1404; Sigma Aldrich) at 3 and 1 days before SARS-CoV-2 infection ...
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bioRxiv - Cell Biology 2023Quote: ... VeroE6 cells were transfected with pHLARE plasmids alone and then after 16 hours were treated with either 200 nM BafilomycinA1 (19-148) from Sigma-Aldrich for 150 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...