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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Cancer Biology 2021Quote: 4-hydroxytamoxifen (4-OHT, Sigma, #7904) was dissolved in 100% ethanol (1 mM stock solution) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-HT [Sigma, H7904]) was dissolved in methanol to a concentration of 5 mg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... or 4-Hydroxytamoxifen (4-OH, Sigma) was dissolved to a final concentration of 1mg/ml or 3mg/ml in 90% corn oil (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1μM 4-hydroxytamoxifen (4-OHT; Sigma) was added to select wells 5 days prior to imaging to allow for adequate Cre-mediated recombination and expression of MCT4 shRNA ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... 4 × 10−4 M monothioglycerol (Sigma), 50 µg/mL ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: 4-PBA (4-phenylbutyric acid, Sigma) and 5-PVA (5-phenylvaleric acid ...
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bioRxiv - Developmental Biology 2019Quote: ... 4-Methylumbelliferone (4-MU, Sigma-Aldrich) was dissolved in DMSO to a stock concentration of 100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-OHT, Sigma, H7904) was dissolved in 100% DMSO at a concentration of 20mg/ml and 25 ul of the 4-OHT solution was injected via intramuscular injection into the left mouse gastrocnemius muscle ...
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bioRxiv - Developmental Biology 2021Quote: 4-hydroxytamoxifen (4-OHT; Sigma H7904) stock was prepared by dissolving the powder in ethanol ...
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bioRxiv - Cancer Biology 2022Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) stock was prepared by dissolving the powder in ethanol to reach 5 or 10 mM concentration and thoroughly vortexed and aliquoted into 100 μl aliquots ...
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bioRxiv - Neuroscience 2022Quote: ... 4 aminopyridine (4-AP, 100µM; Sigma). The currents recorded at the same imposed potential were averaged for each cell to perform the quantification ...
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bioRxiv - Neuroscience 2022Quote: ... 4-aminopyridine (4-AP, Sigma, 275875), and gaboxadol (Sigma ...
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bioRxiv - Immunology 2022Quote: ... 4-hydroxytamoxifen (4-OHT from Sigma) was administered i.p ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma Aldrich) was diluted in ethanol following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-OHT; Sigma, H7904) was dissolved in corn oil/ethanol (90% corn oil ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-HT, Sigma-Aldrich) was dissolved in 100% ethanol (10 mg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... 4-Hydroxytamoxifen (4-OHT; Sigma #H6278) was dissolved in corn oil according to a modification of published methods [24] ...
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bioRxiv - Developmental Biology 2024Quote: 4-hydroxytamoxifen (4-HT, H7904; Sigma) was dissolved in ethanol at a final stock concentration of 5 mM and kept in single-use aliquots in the dark at –80°C ...
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bioRxiv - Developmental Biology 2023Quote: 4-Hydroxytamoxifen (4-OHT, H7904, Sigma) was dissolved in ethanol to final concentration of 10 mM ...
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bioRxiv - Cell Biology 2024Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) was dissolved at 20 mg/mL in ethanol by shaking at 37°C for 15 minutes and was then aliquoted and stored at -20°C for up to several weeks ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) was administrated to 12 weeks old male mice at a dose of 1.0mg/40g body weight via intraperitoneal injection for five consecutive days ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately incubated at 4°C in the primary antibody against the N-terminus of human Fos (overnight, 1:2000; Rabbit polyclonal, ABE457, Millipore; RRID: AB_2631318 (56) (Exp ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein of cell extracts was determined by Bradford method and GST□BHMT was precipitated with glutathione agarose (Sigma). Precipitates were then mixed in SDS□PAGE sample buffer ...
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bioRxiv - Neuroscience 2019Quote: ... The sample was dialysed into TBS overnight and the GST-fusion construct was cleaved using Thrombin (Novagen, Merch 69671). dArc capsids formed spontaneously and the cleaved GST protein was removed by washing the sample over a 100 kDa MWCO spin filtration column (GE vivaspin).
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bioRxiv - Cell Biology 2019Quote: ... GST (clone 8-326, MA4-004 from Thermo Fischer, 1:1000) and Cyclin B (C8831 from Sigma, 1:1000). The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... GST-Cdk5rap2 was purified by affinity chromatography using a glutathione (GSH)-conjugated agarose column (Sigma-Aldrich, St. Louis, MO). Sf9 cell lysates expressing GST-Cdk5rap2 were incubated with GSH-agarose for 1 hour at 4° C and washed with 1x PBS containing 1% Triton X-100 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was spun down at 19,000 rpm for 30 minutes and the supernatant was incubated with GST-Bind resin (Millipore) at 4 °C for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Cancer Biology 2022Quote: ... with the following changes: 90 minute primary antibody incubation at RT (rabbit α-GST, Sigma Aldrich #G7781, 1:4000) and 30 minute secondary incubation at RT (goat α-rabbit HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Cell Biology 2023Quote: ... 15 μg of GST-INCENP wt or S61E were incubated with the indicated amounts of CDK1-CyclinB1 (Sigma #SRP5009) in 50 μL reactions in kinase buffer (50 mM Tris– HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were resuspended in GST-Pull Down equilibration/wash buffer (125 mM Tris-HCl pH 7.5, 150 mM NaCl + Protease Inhibitor Cocktail (Sigma) + 1 mg/ml lysozyme ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Developmental Biology 2024Quote: 10 ug of GST-Nde1-T243A-T246A or GST-Nde1-S214F-T243A-T246A were incubated with or without 0.2 ug active Cdk5 or Cdk1 (Millipore), 400 uM ATP ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...