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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for 6H THIENO 2 3 B THIOPYRAN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... followed by buffer exchange (5 cycles of concentration to 0.5 ml volume and dilution with 5 ml buffer B) using a 3 kDa MWCO Amicon Ultra-15 centrifugal ultrafilter (Millipore), and the purified Alexa647-CAP was stored in aliquots at −80°C.
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bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
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bioRxiv - Physiology 2019Quote: ... heat-polished patch pipettes with tip resistances between 3 and 5 MΩ were used and contained amphotericin B (Sigma-Aldrich) as the perforating agent ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... The presence or absence of bacteria in infected and uninfected samples was checked through staining with Phenolic Auramine O-Rhodamine B dye (4) (1/3 dilution of stock solution) (Auramine O-861020-25gm, Sigma) (Rhodamine B-R6626-100gm ...
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Immunology 2022Quote: ... To asses mitochondrial fuel usage OCR was measured subsequent to the addition of the following drugs in different combinations as indicated: 3 μM bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (SML0601, Sigma), 2 μM UK-5099 (PZ0160 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated for 2 hours with 3 µg/ml 5-bromo-2’-deoxyurine (Sigma-Aldrich, B5002). Cells were then detached using trypsin/EDTA (GIBCO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Biophysics 2021Quote: NmeCas9 was expressed in Rosetta 2(DE3) cells (Millipore Sigma, 71400-3) and grown in LB supplemented with 100 µg/mL Carbenicillin and 34µg/mL Chloramphenicol ...
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bioRxiv - Immunology 2019Quote: ... the MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) powder is re-suspended into PBS ...
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bioRxiv - Neuroscience 2020Quote: ... containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and MiniComplete Protease Inhibitor Cocktail (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2021Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (Sigma-Aldrich) for the MTT assay was dissolved in PBS in stock concentration 5 mg/mL and stored at 4 °C in the dark ...
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bioRxiv - Neuroscience 2022Quote: 2’-3’-dideoxycitidine (ddC) was purchased from Sigma Aldrich (St. Louis, MO). AM1710 was synthesized by the lab of Alexandros Makriyannis (Northeastern University ...
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bioRxiv - Cancer Biology 2022Quote: ... containing phosphatase inhibitor cocktails 2 and 3 and protease inhibitors (Sigma-Aldrich), for 30–45min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... 400μL (3 mg/mL) of methylbenzothiazolinone-2-hydrazone (MBTH, Sigma M8006−1G), 200μL of sample and 0.5μI of alcohol oxidase (E.C ...
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bioRxiv - Biophysics 2019Quote: ... The (-)-1-methyl-2-(3-pyridyl)pyrrolidine was purchased from Sigma-Aldrich Brasil Ltda (Sao Paulo ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 µl (3–4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma) were added to each well and the plate incubated at 37°C for 3 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 µl (3–4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma) were added to each well and the plate incubated at 37°C for 3 h ...
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bioRxiv - Microbiology 2019Quote: ... and [5-(2-thienyl)-3-isoxazolyl]methanol were obtained from Sigma-Aldrich, MO (Table 1) ...
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bioRxiv - Genetics 2020Quote: ... 137.14 mg of 2-methylpyridine-3-carboxylic acid (Sigma Aldrich, cat. 325228) were resuspended in 500 μl DMSO anhydrous (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 2 and 3 emulsified 1:1 in Freund’s incomplete adjuvant (Sigma-Aldrich) on days 0 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µl (3–4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma) were added to each well and the plate incubated at 37°C for 3 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma, 03450) in 50 mM HEPES] for 30 min on slow agitation ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase inhibitors (cocktail 2; P5726; and cocktail 3; P0044; Sigma-Aldrich). Lysates were incubated for 5 min at 100 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich, P5726 and P0044). Homogenized sample was left incubating for disulfide bond reduction for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Spheroids were treated every 2-3 days with either GSK343 (Sigma-Aldrich), GSK126 (Selleckchem) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05 mM (1,4-di [3’-(2’-pyridyldithio)propionamido] butane) (DPDPB, Sigma 16646) in PBS for 5 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 2 and 3 inhibitor (Glutor, 0.05, 0.1, and 0.25 µM, Sigma, SML2765) for 6 to 72 hours ...
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bioRxiv - Neuroscience 2023Quote: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium (MTT) (Millipore Sigma) is converted to water-insoluble product formazan by reduction at mitochondrial complex II of the electron transport chain ...
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bioRxiv - Biochemistry 2024Quote: ... exponentially growing TriEx cells (3 mL suspension, 2×106 cells/mL, Novagen) in serum-free Insect-XPRESS medium (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM PMSF and phosphatase inhibitors cocktails 2 and 3 (Sigma-Aldrich). Lysates were cleared by centrifugation at 17000 x g for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Mice were anesthetized by exposure to 2% to 3% isoflurane (Sigma-Aldrich) for 5 to 10 minutes and intranasally infected with 40 μL of a suspension containing 105 spores for the leukopenic model and 106 spores for the corticosteroid model ...
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bioRxiv - Microbiology 2024Quote: ... and media was replaced every 2-3 days with MEME medium (Sigma), supplemented with 2 mM L-Glutamine (Gibco) ...
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bioRxiv - Synthetic Biology 2022Quote: ... FSL-B (Function-Spacer-Lipid with blood group B trisaccharide) (Sigma Aldrich) or FSL-isoGb3 (Function-Spacer-Lipid with iso-globotriaosyl saccharide).
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bioRxiv - Developmental Biology 2022Quote: ... 2/3 mTeSR1 + 1/3 N2 medium + LSX + PSD Day 6,7: 1/3 mTeSR1 + 2/3 N2 medium + PSD At day 6 cells were detached by Accutase solution (Sigma-Aldrich) and incubated at 37°C for 20 minutes to obtain a single cell suspension ...
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bioRxiv - Molecular Biology 2023Quote: ... were cleaned by sonication with 3 M NaOH then Piranha solution (2:3 ratio of 30% (w/w) H2O2 to sulfuric acid) and treated with 3-glycidyloxypropyl trimethoxysilane (GOPTS) (Sigma). GOPTS was then reacted with a mixture of 9 parts α-hydroxy-ω-amino PEG 3000 to 1 part α-biotinyl-ω-amino PEG 3000 by weight (Rapp Polymere) ...
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bioRxiv - Cell Biology 2023Quote: ... except the protein sample was concentrated to 4 mg/ml and mixed with 0.005% 3-([3-Cholamidopropyl]dimethylammonio)-2-shydroxy-1-propanesulfonate (CHAPSO; Sigma Aldrich) immediately before vitrification ...
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bioRxiv - Developmental Biology 2022Quote: Egg chambers were dissected in phosphate-buffered saline (PBS pH 7.4) + 0.1% Triton X-100 and incubated for 2 hours in 1mM latrunculin B (LatB; Sigma). They were then fixed 20 min in 4% PFA (in PBS pH 7.4) ...
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bioRxiv - Microbiology 2019Quote: ... at 4°C and resuspended in 2 ml of NP40 lysis buffer (1× CellLytic B (Sigma, MO, USA), 50 U DNAse I (Fermentas ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Plant Biology 2022Quote: ... 2012) were selected with 2 μg/ml hygromycin B (Duchefa) or 15 μg/ml PPT (phosphinothricin, Sigma-Aldrich), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were carried out using the indicated antibodies: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), BLM (Bethyl ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Bioengineering 2023Quote: ... b-mercaptoethanol and 1000 U ml-1 LIF) and 2 ug ml-1 doxycycline (cat. 33429 Sigma-Aldrich). MEFs were reprogrammed in the presence of doxycycline for 12 days and without doxycycline for the last 5 days ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody65 (kind gift of G ...
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bioRxiv - Cancer Biology 2021Quote: ... Hygromycin B (Sigma, #H3274) was used at 200 μg/ml to select cells expressing YAP variants.
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bioRxiv - Cell Biology 2019Quote: ... and cytochalasin B (Sigma). All initial stocks were dissolved in 100% dry DMSO and freshly diluted in imaging media before experiments.