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Citations for Millipore Sigma :
3901 - 3950 of 5563 citations for Rat Malonyl Coenzyme A Decarboxylase MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ChIP experiments were performed using EZ-Magna ChIPTM A/G Chromatin Immunoprecipitation Kit (Merck Millipore, 17-10086) according to the manufacturer’s procedures ...
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bioRxiv - Cell Biology 2022Quote: Hydroxyproline content was determined according to the manufacturer’s instructions (Hydroxyproline Assay Kit, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... The specific steps were carried out according to the instructions of a TUNEL kit (Merck Millipore, USA). Five regions were randomly selected from the ischemic penumbra of each brain slice under a 400× magnification objective lens (Olympus ...
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bioRxiv - Molecular Biology 2019Quote: Apoptosis was determined using the Annexin V-PI or Annexin V-7AAD Apoptosis Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: DNA isolations were performed using the GenElute™ Plant Genomic Miniprep Kit (Sigma-Aldrich, St. Louis, MO). DNA quality and quantification were determined using a Nanodrop ND-1000 (Nanodrop Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... harvested to prepare nuclear extract using the CelLytic™ NuCLEAR™ Extraction Kit (Sigma, Missouri, Unites States). For luciferase reporter assays ...
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bioRxiv - Systems Biology 2019Quote: ... Nuclei were washed once with 15 ml Nuclear Isolation Buffer from the CelLytic PN kit (Sigma-Aldrich) and re-pelleted ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The medium alanine aminotransferase (ALT) activity was quantified using the ALT assay kit (MAK052, Sigma-Aldrich Corp.).
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bioRxiv - Pathology 2019Quote: ... Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by using the spectrometric kits (Sigma Diagnostics). Triglyceride ...
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bioRxiv - Cell Biology 2019Quote: ... Cultured cells and parasites were regularly screened against mycoplasma contamination using the LookOut Mycoplasma detection kit (Sigma) and cured with Mycoplasma Removal Agent (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DHFR activity and screening of DHFR inhibitors testing was examined using the Dihydrofolate Reductase Assay Kit (Sigma). The assay is based on the ability of DHFR to catalyze the reversible nicotinamide adenine dinucleotide phosphate (NADPH ...
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bioRxiv - Molecular Biology 2019Quote: Telomerase activity was detected using the PCR-based Telomeric Repeat Amplification Protocol (TRAP) assay kit (Millipore, S7700). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... The proximity ligation assay was conducted using a DuoLink In Situ Mouse/Rabbit PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was extracted from the collected samples using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and was treated with RNase-free DNaseI (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining for apoptosis was performed using the Apoptag Fluorescein in situ Apoptosis Detection Kit (Millipore; S7110) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich) using a Fluoroskan Ascent fluorescence multiwell plate reader (Thermo Labsystems) ...
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bioRxiv - Pathology 2020Quote: ... Serum Triglyceride and serum free glycerol were quantified using Serum Triglyceride Determination Kit (Sigma-Aldrich, cat# TR0100) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... All constructs were prepared for transfection using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2020Quote: ... Residual concentrations of formate were checked by a Formate Assay Kit following the manufacturer’s protocol (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... Genotyping PCR was performed from genomic DNA from tails using standard protocol of N-Extract kit (Sigma).
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bioRxiv - Microbiology 2020Quote: Total genomic DNA was extracted using the REDExtract-N-Amp™ Tissue PCR Kit (Sigma- Aldrich, USA), which yields high quantities of DNA ...
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bioRxiv - Plant Biology 2021Quote: ... RNA from 100 mg of leaf tissue was extracted using the Spectrum Total RNA kit (Sigma-Aldrich). An on-column DNase digestion (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... sTRAP assay was measured with acetate buffer from acid phosphatase leukocyte kit (Sigma-Aldrich, St. Louis, MO), 1M sodium L-tartrate dibasic dihydrate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... following incubation using materials from the PKH26 Red Fluorescent Cell Linker Kit (Millipore Sigma, St. Louis, MO), the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... the chloroplasts were first isolated from the intact leaves using a chloroplast isolation kit (Sigma-Aldrich, USA), after which further intact chloroplasts were harvested using a 40/80% Percoll gradient ...
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bioRxiv - Physiology 2020Quote: Proximity ligation assay was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmids were extracted and purified using the GenElute plasmid miniprep kit (Sigma-Aldrich, St. Louis, MO, USA). EcoRI and KpnI cut sites were introduced using restriction enzyme cut-site primers (Supplementary file 3 ...
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bioRxiv - Cell Biology 2021Quote: The glycerol content of cell culture media was quantified using a Glycerol Assay Kit (Sigma Aldrich MAK117) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested backbone was purified from 0.7 % Agarose Gel using the GenEluteTM Gel extraction Kit (Sigma Aldrich, NA1111). Vector and miRNA target inserts were ligated at 1:10 molar ratio using 400 U of T4 DNA Ligase (NEB ...
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bioRxiv - Genetics 2020Quote: ... nuclei were enriched from the Dounce-homogenized brain tissues using Nuclei EZ prep Kit (Sigma, NUC-101). DNA were digested with micrococcal nuclease (MNase ...
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bioRxiv - Neuroscience 2021Quote: Glutamate quantity in hippocampal homogenates (10 μl) was measured colorimetrically utilizing Glutamate Assay Kit (Sigma-Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Proximity ligation assays were performed using the Duolink® flowPLA Detection Kit – Orange (Millipore Sigma, Cat. DUO94003) according to manufacturers recommended protocol with minor changes ...
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bioRxiv - Physiology 2021Quote: ... paraffin sections were dewaxed and rehydrated and stained using an Elastic Stain Kit (Millipore, HT25A, Burlington, MA). Brightfield imaging was done on a Nikon E600 microscope with Nikon NIS-Elements software ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of digoxigenin-labeled probes was performed using the DIG RNA Labeling Kit (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was extracted using the GenElute mammalian total RNA miniprep kit from Sigma (Sigma, RTN350-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was performed using manufacturer’s instructions for the EZ-Nuclear RIP Kit (EMD Millipore, #17-10 523), followed by cDNA synthesis and qRT-PCR for 5’ETS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Evolutionary Biology 2020Quote: All Digoxygenin (DIG) labeled probes were generated using the Roche DIG labeling kit (Sigma Cat no 11277073910). Antisense probes were generated using Pme1 (NEB cat no R0560S ...
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bioRxiv - Cell Biology 2020Quote: ... were constructed by the ligation-independent cloning method using a pET-41 Ek/LIC vector kit (Novagen). The coding sequences of human LC3B and GATE-16 proteins were amplified by PCR with KOD-Plus-Neo DNA polymerase (Toyobo) ...
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bioRxiv - Immunology 2020Quote: ChIP assays were performed with an EZ ChIP kit purchased from Upstate Biotechnology (Millipore Corporation Billerica, MA). Briefly ...
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bioRxiv - Immunology 2020Quote: Deglycosylation of Bl-Eng2 was performed by using the GlycoProfile(tm) IV Chemical Deglycosylation kit (Sigma-Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... to be further randomly amplified using a modified Whole Transcriptome Amplification 2 (WTA2) kit procedure (Sigma-Aldrich). The products were purified ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA extraction was performed with a REDExtract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... paraffin-embedded tissue sections (3-mm) using commercial Masson’s trichrome staining kits (Sigma–Aldrich, St. Louis, MO) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The amplified hoc products were gel-extracted following manufacturer’s protocol (GenElute™ Gel Extraction Kit, Sigma Aldrich). Wildtype and D246N hoc genes were individually cloned in-frame to expression vector pPROEX-HTb ...