Labshake search
Citations for Millipore Sigma :
3851 - 3900 of 7667 citations for Recombinant Ovine IL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were ultrafiltrated using 10 K pore size Amicon Ultra filter units (Millipore) and step-wise dialyzed against 5 mM Pipes-Na pH 7.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was induced with 0.25 mM IPTG in E.coli Rosetta BL21(DE3) (Novagen) in the presence of 50 µM ZnCl2 at 37° C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
-
bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 20 μM ...
-
bioRxiv - Biochemistry 2021Quote: ... Pure protein was concentrated using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa MWCO) to around 55 μM ...
-
bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Cell Biology 2021Quote: ... The secreted S proteins were purified by anti-FLAG M2 affinity resin (Sigma Aldrich). After loading two times ...
-
bioRxiv - Cell Biology 2021Quote: ... we inhibited global protein synthesis with cycloheximide (CHX, 100 μg/mL, Sigma Aldrich # C4859).
-
bioRxiv - Molecular Biology 2021Quote: ... Total proteins were extracted from transfected 293T cells using RIPA lysis buffer (Millipore Sigma) at 48 hpt ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 20 mg of protein was mixed with 2x Laemmli buffer (Sigma Aldrich, #S3401) and boiled for 5 min at 95°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were then electrotransferred to an Immobilon®-P polyvinylidene fluoride (PVDF) membrane (Millipore). Membrane was blocked with 5% non-fat milk ...
-
bioRxiv - Cell Biology 2019Quote: ... the purified protein was concentrated over a 10k Amicon Ultra-4 Centrifugal filter (Millipore) then immediately aliquoted ...
-
bioRxiv - Genomics 2021Quote: Proteins were separated using 6-15% SDS-PAGE and transferred onto PVDF membranes (Millipore). Then ...
-
bioRxiv - Biophysics 2020Quote: ... The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff, Millipore) and further purified using Superose 6 Increase 10/300 GL column (GE Healthcare).
-
bioRxiv - Cancer Biology 2021Quote: ... Protein transfer was checked by staining the membrane with Ponceau S red (Sigma-Aldrich). The membrane was then blocked using 5% bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 μL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
-
bioRxiv - Genetics 2021Quote: ... Proteins were blotted from the gel onto polyvinylidene difluoride (PVDF) membranes (Immobilon, EMD Millipore) and detected using primary and secondary antibodies ...
-
bioRxiv - Immunology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted according to protocols described before (42) ...
-
bioRxiv - Microbiology 2021Quote: The proteins in the elution were concentrated by Amicon Ultra centrifugal filters (Millipore Sigma) and separated briefly in a 10% Bis-Tris SDS-PAGE gel for 10 min ...
-
bioRxiv - Microbiology 2021Quote: ... The resultant protein bands were visualized after staining with Ponceau-S (Sigma-Aldrich, USA). Next ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Purkinje cell protein 4 (PCP4; Sigma, dilution 1:400), rabbit polyclonal antibody raised to Capn15 (Zha ...
-
bioRxiv - Immunology 2020Quote: ... ABCF1 protein was probed with primary anti-ABCF1 antibody (HPA017578, Sigma-Aldrich, Oakville, Ontario) at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Excess Protein-PEG2000-DPSE was separated from liposomes using a CL-4B Sepharose (Sigma) column ...
-
bioRxiv - Neuroscience 2020Quote: ... Synaptic proteins were detected mouse anti-PSD95 (1:500, MAB1598, Millipore, Burlington, MA, U.S.A.), guinea pig anti-VGLUT1 (1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary and secondary antibodies were diluted as follows: polyclonal rabbit-anti-Protein A (Sigma) detecting the PTP epitope 1:2000 ...
-
bioRxiv - Immunology 2021Quote: Actin was used as a housekeeping protein (Rabbit anti-Actin antibody (Sigma-Aldrich A2066) followed by Goat anti-Rabbit-HRP (Southern Biotech 4050-05)).
-
bioRxiv - Molecular Biology 2020Quote: ... and then with protein A agarose/salmon sperm DNA slurry (Millipore, Cat# 16-157). The beads were washed and eluted as described43 ...
-
bioRxiv - Biophysics 2020Quote: ... The protein was then eluted with lysis buffer containing imidazole (Sigma-Aldrich Co., USA) at different concentrations (20 mM ...
-
bioRxiv - Microbiology 2022Quote: ... The protein samples were concentrated by ultrafiltration using an Amicon Ultra-3KDa concentrator (Millipore). For further purification ...
-
bioRxiv - Neuroscience 2022Quote: ... Soluble proteins were then incubated with 1 μg of anti-FLAG antibody (F1804, Sigma) or 1 μg of mouse IgG (sc-2025 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein lysate was then blotting on a polyvinylidene fluoride (PVDF) membrane (Millipore, MA, USA) and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies.
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra-10k centrifugal filter device (Merck Millipore Ltd.), and the concentration was determined using Bradford assay kit (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected and pre-cleaned with Protein G Agarose beads (Sigma-Aldrich) for 1-2 h at 4 °C rotating to enhance specificity and the total protein content was quantified by Bradford assay ...
-
bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2022Quote: We conducted SEC on the SEC protein standards (molecular weights 12.4-200 kDa; Sigma) including β-amylase from sweet potato (200 kDa) ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was concentrated by centrifugal filtration (Amicon, EMD Millipore, 10-kDa MW cutoff) at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein recovery was maximized by adjusting the concentration of imidazole (Sigma cat# l5513-5G) as follows ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were transferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Sigma-Aldrich) at 100V for 1 hour on ice in NuPAGE Transfer Buffer (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-FL Transfer Membrane (Millipore). The membranes were blocked in PBS-T (PBS containing 0.05% Tween 20 ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: Total cell protein was extracted with lysis buffer (RIPA, Sigma-Aldrich, catalog no. R0278) containing 1% protease-phosphatase inhibitor cocktail (Thermo Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Proteins separated by SDS–PAGE were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100 V for 1 h at 4 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
-
bioRxiv - Microbiology 2022Quote: ... the total protein concentration of each sample was measured via Bradford assay (Sigma Aldrich). Samples were subsequently diluted to a final concentration of 0.5 µg total protein/µl ...
-
bioRxiv - Microbiology 2022Quote: ... The TS fraction was concentrated using Amicon protein concentrators with a 10KDa cutoff (Sigma).