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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... (1) A modification solution was made by dissolving 10 mM of 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC, Sigma-Aldrich Inc.) and 75 mM of N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... BSA was coupled to the PEG (3000) for 1h at RT with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 100 mM, Sigma Aldrich, 39391) and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2019Quote: ... were treated for 20 min at room temperature (RT) with 200 µl of Bind-silane solution—a mixture of 714 µl 3-(Trimethoxysilyl)propyl methacrylate (3-TMP, Sigma-Aldrich, M6514), 714 µl of acetic acid in 10 ml of 96% ethanol ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Neuroscience 2019Quote: ... samples were washed 3 × 3 minute with PBS followed by a second 30-minute fixation step in 1% osmium tetroxide (Sigma-Aldrich, 75632). A final 3 × 3 minute PBS wash step was performed before samples were serially dehydrated in ethanol with a final 30-minute dehydration step using hexamethyldisilazane (HMDS ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Neuroscience 2020Quote: ... FBS supplementation was decreased to 3% FBS 3 days prior to the addition of 10□M Retinoic Acid (RA; Sigma-Aldrich, R2625). During differentiation ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 (15 μg/mL) was immobilized on AR2G biosensors activated by 1-ethyl-3-[3-dimethylaminopropy] carbodiimide hydrochloride (EDC, E1769, Sigma, Shanghai, CHN) and sulfo-N-hydroxysulfosuccinimide (s-NHS ...
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bioRxiv - Microbiology 2021Quote: Viral stocks of molecular clones HIV-1NL4-3 and HIV-1NL4-3 BaL (AIDS Reagents Program) were generated by transfecting HEK293T cells using PEI transfection reagent (Sigma, Cat: 408727). Pseudotyped HIV-1VSVg-NL4-3 ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary guide oligonucleotides (forward: 5’-CACCGCAGGATTGAAGACCTTAACA-3’; reverse: 5’-AAACTGTTAAGCTCTTCAATCCTGC-3’) were custom synthesized separately by Sigma-Aldrich (St. Louis, MO, USA), annealed ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the AgNP suspension was washed with deionized (DI) water by ultrafiltration through a membrane with pore size of 3 kDa (Amicon Ultracel 3 KDa Ultrafiltration Discs, Millipore, Billerica, USA) under nitrogen gas pressure (1 bar ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hrs followed by addition of 3 µM Asunaprevir (Apexio: BMS-650032) and 500 µM 3-Indoleacetic acid (Sigma Aldrich: I2886). Cells were treated with following inhibitors to inhibit indicated kinases ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were probed overnight at 4°C with anti-HA antibody (MBL, M180-3) and anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Sigma-Aldrich, G8795) or with anti-dystrophin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... A 0.5 M stock solution of inducer was prepared by solubilizing 3-methyl-benzoate (3-MB) (Sigma-Aldrich, St Louis, MO, USA) in 0.5 M NaOH.
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bioRxiv - Systems Biology 2022Quote: ... The His6-MBP or His6-MBP-14-3-3 concentrations on the resin were determined by pipetting 3×200 μL of diluted resin (corresponding to 3×15 μL of resin) into a filter plate (Millipore, Burlington, Massachusetts), filtering out the buffer by centrifugation (500g) ...
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bioRxiv - Genetics 2023Quote: ... Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 µg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Chemical compounds were first identified using the NIST library and later confirmed with co-elution of synthetic 4-methyl-3-heptanone (Pfaltz and Bauer M19160) and 4-methyl-3-heptanol (Sigma Aldrich M48309). Compounds eluting after 30 minutes were excluded from the analysis due to lack of volatility.
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bioRxiv - Developmental Biology 2023Quote: ... oocytes are first isolated from ovaries by short enzymatic digestion (3 mg/ml Collagenase I, Sigma-Aldrich, #C0130; 3 mg/ml Collagenase II, Sigma-Aldrich, #C6885 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were loaded onto a Supelco™ Discovery™ HS F5-3 (15 cm×2.1 mm, 3-μm) column (Sigma-Aldrich Co.) kept at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 8029.3), and 0.5% sodium deoxycholate (AppliChem, A1531) in PBS supplemented with Phosphatase Inhibitor Cocktail 2 and Cocktail 3 (Sigma-Aldrich, P5726, P0044) and Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Immunology 2024Quote: ... The extract was reconstituted in a 500-μl solution of methanol/water (1:1) and filtered through a 3-kDa molecular filter (Amicon® Ultra Centrifugal Filter, 3 kDa MWCO, Millipore Sigma) at 4°C for 90 minutes to remove proteins ...
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bioRxiv - Plant Biology 2019Quote: ... 20 μL/mL Phosphatase Inhibitor Cocktail 3 (Sigma, P0044), 20 μL/mL Phosphatase Inhibitor Cocktail 2 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and Pellet Paint Co-precipitant (Merck Millipore #69049-3). Genomic DNA traces were removed with DNA-free DNase Treatment and Removal Reagents (FisherScientific #AM1906 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μM 3-Isobutyl-1-methylxanthine (IBMX, Sigma, #I7018) was supplemented to the media for the indicated time ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% of phosphatase inhibitor cocktail 3 (Sigma-Aldrich) was added to the adherent cells and permitted to incubate on ice for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides carrying a 3’ Cy5 fluorophore (purchased from Sigma) were ligated subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich), for 1 hour at 4°C on rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 3 μl of 8 mg/ml hexadimethrine bromide (Sigma) was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-γ-adaptin (100/3) from Sigma. Hybridomas producing antibodies against LDL receptor (C7 ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in 3% H2O2 (Sigma-Aldrich, Shanghai, China) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 3-methyl-2-oxovaleric acid sodium salt (ketoisoleucine, Sigma) or a combination of all three (BCKAs) ...