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Citations for Millipore Sigma :
3851 - 3900 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
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bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
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bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from human RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Sigma). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
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bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
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bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
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bioRxiv - Microbiology 2024Quote: ... PBMC were adjusted at a density of 106 cells/mL in RPMI supplemented with 10% human AB serum (Sigma-Aldrich) and seeded in 96-well culture plate coated with collagen at 10 µg/cm2 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... High-binding 96-well microtiter plates (Costar, Cambridge, MA) were coated overnight at 4°C with a goat anti-human IgG Fc antibody (Sigma) at 3 µg/mL in Carbonate-Bicarbonate buffer (0.1 M ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed three times with 0.25% (w/v) saponin and subsequently blocked with 1X PBS containing 10% (v/v) human serum (Sigma Aldrich) and 0.25% (w/v ...
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bioRxiv - Physiology 2024Quote: ... The induction medium consisted of DMEM/F-12 (+10 % FBS, +1 % PenStrep) supplemented with 340 nM human insulin (Sigma-Aldrich), 1 µM dexamethasone (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.4) and Tris-buffered saline (TBS, pH 7.4) and blocked in a solution of 1% human serum albumin (Sigma-Aldrich) and 0.1% Triton-X (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 30-day-old human neuronal cultures were exposed to various doses of Aβ-42o (Sigma-Aldrich, St. Louis, Missouri, USA), ranging from 0.1 µM to 5 µM ...
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bioRxiv - Immunology 2024Quote: ... Both human and mouse islets were hand-picked and dispersed using a non-enzymatic dispersion solution (Sigma-Aldrich, Cat #: C5914) for 3 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... The experiment was conducted after compound treatment of either cell lysates (Xenopus laevis oocytes, human astrocytes, whole brain lysate) in 0.4% NP40 PBS (Sigma-Aldrich) or living human astrocytes (ScienCell ...
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bioRxiv - Biophysics 2024Quote: ... at a density of 6 × 105 cells/ml in 400 ml of glutamate-free RPMI supplemented with 20% FBS under constant agitation of 60 rpm for 4-5 days and in the presence of 2.5 μg/ml anti-human IgM antibody (Sigma-Aldrich) to induce the KSHV lytic cycle ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed with PBS-T and 25 μL of detection antibody diluted in assay buffer added to each well: goat anti-human IgG-AP (1:1,000) (Sigma Aldrich) or goat anti-human IgG3-AP (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... Samples were evaluated using the MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A Magnetic Bead Panel (Millipore HCYTA-60K-PX48). Creatinine concentration was measured using the Creatinine Parameter colorimetric assay (R&D Systems KGE005) ...
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bioRxiv - Immunology 2024Quote: ... human memory Th cells were labelled with 0.05 mM carboxyfluorescein diacetyl succinimidyl ester (CFDASE) cell proliferation reagent (Millipore Sigma Aldrich) according to published protocol (26) ...
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bioRxiv - Bioengineering 2024Quote: ... Fresh human subcutaneous lipoaspirate from patients undergoing elective liposuction surgery was processed by digestion in collagenase Type IA (Sigma Aldrich), and then density centrifugation was used to isolate the stromal vascular fraction ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 cells were treated with esiRNA targeting human HSC70 or firefly luciferase as control for 72 hours prior to the experiment (Sigma, human HSC70 ehu115141 ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts) and anti-CD68 mouse monoclonal antibody (used for immunofluorescence in human tissue samples) were from Millipore/Sigma (cat ...
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bioRxiv - Cancer Biology 2024Quote: The human ETV6::RUNX1 Reh B-ALL cell-line (DSMZ #ACC 22;,) was maintained in RPMI-1640 medium (Sigma #R0883) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Cell Biology 2024Quote: HEK293 cells (human embryonic kidney cells) and βarr1/2 knockout cells were maintained as a monolayer in T-75 cm2 flasks (Sigma). βarr1/2 knockout HEK293 cells were made by using CRISPR-Cas9 (kindly provided by Asuka Inoue ...
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bioRxiv - Cell Biology 2024Quote: ... bacterial filaments were opsonized by resuspending the bacterial pellet in a 4 mg/ml solution of human IgG (Sigma-Aldrich) in PBS and allowing the sample to mix on a rotator overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: The human colorectal adenocarcinoma cell line Caco-2 (HTB-37; ATCC) was grown in DMEM with 20% FBS (F1051-500mL; Sigma), 1% sodium pyruvate (S8636-100mL ...
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bioRxiv - Developmental Biology 2024Quote: ... The following day the zona pellucida of the 2-cell stage human embryos was removed by brief acidic Tyrode’s solution treatment (Sigma, T1788).
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bioRxiv - Developmental Biology 2024Quote: To generate human and mouse unguided brain organoids single cell suspensions were obtained through incubations in Accutase (Sigma-Aldrich, A6964) for 4min at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: Human HEK293T cell line was grown in Dulbecco’s Modified Eagle’s Medium containing high glucose with HEPES modification (Sigma-Aldrich®, D6171), supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Murine or human differentiated brown adipocytes were fixed with 4% PFA and stained with 0.3% Oil Red O solution (Sigma-Aldrich, O0625). The images were observed under a Nikon microscope.
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were selected from the “in-human” collection docking screen and purchased from different vendors: Pterin (Sigma-Aldrich, P1132), Verdiperstat (MedChem Express ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Developmental Biology 2020Quote: Female 7-12-week-old FVB/NJ mice were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PSMG) (ProSpec HOR-272) followed by 5 IU human chorionic gonadotropin (hCG) (Sigma CG10) 48 hours later.
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Cell Biology 2021Quote: ... in the presence of HODHBt or DMSO control and cultured in complete RPMI 1640 medium containing 10% human AB serum (Sigma-Aldrich). As a control ...
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[18F]DCP, First Generation PET Radiotracer for Diagnosis of Radiation Resistant Head and Neck CancerbioRxiv - Cancer Biology 2020Quote: The ex vivo serum stability of the radiotracer [18F]DCP was performed following previously published methods (55–57) and human serum (Sigma Aldrich). [18F]DCP (~1-2 mCi ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines stably expressing shRNA to human FBXO7 were generated as described previously (33-35) and selected with 2 µg/mL puromycin (Sigma-Aldrich). To vary glucose concentration ...