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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Endotoxins were removed from the plasmids using the Endotoxin removal Solution (Sigma, E4274-25ML).
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bioRxiv - Microbiology 2019Quote: Chimeric phage was generated by homologous recombination using plasmid pNL9164 (Sigma-Aldrich, MO, USA) in S ...
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bioRxiv - Synthetic Biology 2020Quote: The creation of all bacterial IgG expression constructs involved plasmid pCOLADuet™-1 (Novagen), which is designed for the coexpression of two target genes from independent upstream T7 promoter/lac operator regions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Variants were subcloned from the pEDA3 background into protein expression plasmid pET-29b(+) (Novagen) or into the constitutive expression plasmid pEDA2 (Wrenbeck et al ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Immunology 2019Quote: ... The purified plasmid was transformed into S2 cells grown in EX-CELL 420 (Sigma) with 25 μL ExpreS2 Insect-TR 5X (ExpreS2ion Biotechnologies) ...
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bioRxiv - Biochemistry 2019Quote: ... The purified plasmid was transformed into S2 cells grown in EX-CELL 420 (Sigma) with 25 μL ExpreS2 Insect-TR 5X (ExpreS2ion Biotechnologies) ...
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bioRxiv - Microbiology 2019Quote: ... strains harbouring pGCP123 plasmids were grown in 1000 µg/mL kanamycin (Sigma Aldrich, USA) and strains harbouring pMSP3535 plasmids were grown in 100 µg/mL erythromycin unless stated otherwise ...
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bioRxiv - Genetics 2021Quote: The Csm2-Psy3 heterodimer was cloned into the dual expression plasmid pRSFDuet (EMD Millipore) which encode a Strep-tagged Csm2 and FLAG-tagged Psy3 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.8 µg plasmid expressing HIV-1 gag-pol using X-tremeGENE 9 (Sigma). Media was changed 6 h later and lentivirus containing culture supernatants were collected at 48 and 72 h post-transfection ...
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bioRxiv - Biochemistry 2020Quote: The phytochrome from Deinococcus radiodurans strain R1 (DrBphP, gene DR_A0050) in pET21b(+) plasmid (Novagen) was kind gift from Prof ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid expression was induced with 1 mM IPTG (Sigma-Aldrich, St. Louis, MO, USA) and the culture was grown further at 16 °C overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Bacteria were grown overnight with the plasmid of interest in Lysogeny Broth (Sigma-Aldrich) and the respective antibiotics ...
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bioRxiv - Biochemistry 2021Quote: ... a high-copy plasmid from a different incompatibility group (pBR223 origin of replication; Novagen) containing the lacIq repressor ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting PCR fragment and the Plasmid DNA of the overexpression vector pET28a(+) (Novagen) were digested with NdeI and BamHI ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... strains harbouring pMSP3535 plasmids were selected using 100 ug/mL erythromycin (Sigma Aldrich, USA) and induction of gene expression was performed using 5 ug/mL nisin from Lactococcus lactis (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... the construct plasmid was transformed into BL21(DE3)pLysE bacteria (Sigma-Aldrich, CMC0015-20X40UL), and grown in TB supplemented with 100 µg/mL ampicillin and 33 µg/mL chloramphenicol at 37 °C until an OD600 of 0.4 was reached ...
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bioRxiv - Microbiology 2022Quote: ... 25 µg of viral plasmid and 100 µl branched PEI (pH 7.4) (Sigma-Aldrich) were added to the cell culture ...
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bioRxiv - Biochemistry 2022Quote: ... The expression plasmid was transformed into Rosetta-Gami®2 (DE3) competent cells (Novagen) to maintain disulfide bond formation ...
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bioRxiv - Microbiology 2021Quote: ... Up to 12 µg linearised plasmid DNA was added to an electroporation cuvette (Sigma), and 100 µL cells were subsequently added ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid vectors were transformed in BL21(DE3) competent cells (Invitrogen or Sigma Aldrich) and grown overnight in a starter culture of 50 mL LB media (Fisher Bioreagents ...
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bioRxiv - Molecular Biology 2019Quote: ... bacterial strain BLR (DE3) was co-transformed with pLacRARE2 plasmid from Rosetta2 strain (Novagen), GST-p125 and pCOLA-hPold234 ...
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bioRxiv - Molecular Biology 2019Quote: ... WT and mutated DEF6 plasmids were transfected (Gene-juice Transfection Reagent, Novagen, #70967-3) into COS7 cells and 24h after the transfection ...
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bioRxiv - Developmental Biology 2021Quote: Zinc finger nuclease (ZFN) plasmids targeting the LEPTIN gene were designed by Sigma-Aldrich. Fetal fibroblasts were isolated from 30-day old male pig embryo ...
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bioRxiv - Cell Biology 2020Quote: ... We used a previously reported plasmid (McMahon et al., 2012) with the pET21b (Novagen) backbone that expresses a C-terminal truncation of Sec12 (Sec12ΔC ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were sub-cloned into the plasmid expression vector pET-28a (Novagen) previously cut with the restriction enzymes EcoR I and Xho I ...
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bioRxiv - Immunology 2022Quote: ... the plasmid DNA was added with transfection reagent and serum-free DMEM (Sigma-Aldrich,) to cells for 24 h by strictly following manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The above plasmids were co-transfected into brown preadipocytes and selected with puromycin (Sigma). Cells were re-plated without puromycin and single cell clones were examined by genotyping ...
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bioRxiv - Molecular Biology 2022Quote: ... the plasmids were used to transform BL21 (DE3) chemically competent cells (Sigma-Aldrich-Aldrich). After transformation and growing the transformed cells on antibiotics selection agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’-pGEM-T construct was then isolated with GenEluteTM Plasmid miniprep kit (Sigma) and digested with 0.5 µl (10 U ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasmids were concentrated using an Amicon spin centrifugal filter unit (Millipore Sigma). A small portion of these plasmids was also prepared with 32 Illumina library indexes and sent for sequencing along with the output library.
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bioRxiv - Cancer Biology 2024Quote: ... The sgRNA plasmid was then transfected into 4T1 cells using Nanojuice transfection reagent (Millipore). After 48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transfected with plasmid encoding Flag-DYRK1B using PEI Prime (Sigma-Aldrich), then 4 h later medium was changed and cells were left for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... The subcultures of strains carrying pBAD33 plasmids were grown with 0.2% arabinose (Sigma-Aldrich). OD600 was measured ...
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... EGFP sequence cloned into the pIEx-4 plasmid (Sigma-Aldrich, Saint Louis, MO, USA) served as a negative control for cell line selection.
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bioRxiv - Cell Biology 2024Quote: ... The cloned plasmids were transfected into HEK293T cells using GeneJuice transfecting agent (Merck Millipore). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... MPP6 and NO66 were PCR cloned from plasmid DNA or cDNA into pFLAG (Sigma), pEF6 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... coli XL-I Blue cells with the Sigma GenElute Plasmid Miniprep Kit (Sigma-Aldrich) as specified by the manufacturer.
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bioRxiv - Genetics 2023Quote: ... plasmids were transformed into Rosetta2(DE3) Plyss SinglesTM competent cells (Millipore Sigma, Catalog #70236), and individual colonies were chosen and optimized for protein expression during the following day ...
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bioRxiv - Physiology 2023Quote: ... All vectors were prepared with the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (Sigma). On day 0 ...
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bioRxiv - Biophysics 2023Quote: ... packaging plasmids at a 1:1:1:2 molar ratio using polyethyleneimine (Sigma-Aldrich). Viral supernatant was collected 48 and 72 h post transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... Pre-validated CompoZr(R) ZFN plasmids for Il2rg (CKOZFN33739) were purchased from Sigma-Aldrich. Il2rg ZFN mRNAs were synthesized by in vitro transcription with mMESSAGE mMACHINE T7 Ultra kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilized cells were incubated with the specified primary antibodies (mouse monoclonal anti-acetylated tubulin T6793, Sigma-Aldrich; and mouse monoclonal anti-FLAG M2 antibody, F1804, Sigma-Aldrich) for 1 h washed with PBS ...
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bioRxiv - Physiology 2019Quote: ... and incubated for 2 h at room temperature with anti-myosin heavy chain I antibody (MHCI; mouse IgM, Developmental Studies Hybridoma Bank: reference #A4.480) and anti-dystrophin antibody (mouse IgG2b, Sigma Aldrich: reference #D8168) in 5% goat serum diluted in PBS (1:1 PBS dilution) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were probed with anti-mouse-AID (1/25, eBioscience, 14-5959-82) and anti-mouse-tubulin (1/5000, Sigma-Aldrich, T9026). Then ...
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bioRxiv - Neuroscience 2020Quote: ... one was reacted against anti-calbindin antibody raised in mouse (Swant Inc., Marly, Switzerland) and another against anti-NeuN antibody raised in mouse (EMD Millipore, Germany). The remaining series was reacted with either anti-parvalbumin antibody raised in mouse (Swant Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Neuroscience 2023Quote: Cytokines from mouse brain homogenates prepared in section 2.7 were measured using Millipore mouse cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...