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Citations for Millipore Sigma :
3851 - 3900 of 8634 citations for BD 3 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Neuroscience 2023Quote: ... thoracic and lumbar dorsal root ganglia neurons were extracted from E17.5-E18.5 rat embryos and plated on glass coverslip coated with poly-L-ornithine (Sigma-Aldrich, MO, USA) for 4-6 hours before experiments.
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were dissected from postnatal Sprague Dawley rats (P0-P2) and dissociated with 0.25% trypsin (Sigma Aldrich, Merck, Darmstadt, Germany) and 0.004% DNAse (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were incubated overnight at 4°C with gentle shaking with rat anti-DAT (1:1000, Millipore Cat# MAB369, RRID:AB_2190413), rabbit anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... After 1-2 months old rats undergo trans cardiac perfusion with PBSx1 (02-023-5A, Biological Industries) followed by neutral buffered 10% formalin (HT5011, Sigma-Aldrich). The whole brain was removed and immersed in 4% paraformaldehyde (PFA ...
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bioRxiv - Physiology 2023Quote: ... the supernatant and the fasting and 15 min GTT serum was performed using Rat/Mouse Insulin ELISA kit (EZRMI-13K, Sigma-Aldrich).
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bioRxiv - Bioengineering 2023Quote: Decellularized rat lung and isolated lobes were perfused with a solution of 0.1% Methylene blue solution (Cat. # 03978, Sigma Aldrich, UK) in PBS either from the trachea or pulmonary artery ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse and rat kidneys were fixed by perfusion with 50-60 mL of 4% paraformaldehyde in PBS (158127, Sigma-Aldrich), dehydrated and embedded in paraffin at 58C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Cell Biology 2023Quote: Human EndoC-βH1 and rat INS1E beta cells were cultured as previously described [45] and incubated with lentiviruses expressing an NNAT-targeting shRNA (Sigma-Aldrich) or Silencer Select siRNAs (Ambion) ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti- cytokeratin 8 (TROMA-I; Developmental Studies Hybridoma Bank; 1:500) and rabbit anti- Sox9 (Millipore, Burlington, MA; 1:750). Detection of antibody binding was with the appropriate species-specific Biotin-SP-conjugated goat anti-antibodies (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... BMDMs were derived on untreated plastic plates (BD Falcon, Sigma-Aldrich) by culturing in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... strains were streaked from frozen stock onto Bacto agar (BD, Sigma) plates containing LB (Miller ...
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bioRxiv - Molecular Biology 2019Quote: Plasma RNA was extracted using TRI Reagent BD (T3809, Sigma Aldrich), in accordance with manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 2-3-week-old plants were treated with 150µM DCMU (D2425-100G, Sigma), kept for 1 hour in the dark and then exposed to high light (HL ...
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bioRxiv - Biophysics 2021Quote: ... Ultrapure water from a Direct-Q 3 UV water purification system (Millipore, USA) was used for all experiments.
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bioRxiv - Cell Biology 2020Quote: ... and 3 μM CHIR99021 on 0.1% gelatin (Catalog No. ES-006-B, Millipore)-coated plates (for the 2i condition) ...
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bioRxiv - Cell Biology 2019Quote: ... Fractions were analyzed by immunoblotting using an antibody to PC1/3 (Sigma, #SAB1100415). Band intensity was quantified using FIJI and plotted against the sucrose concentration of the fraction.
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bioRxiv - Cell Biology 2019Quote: ... all coverslips were incubated with a 5% 3-aminopropyltrimethoxysilane solution (281778, Sigma-Aldrich) in water for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: Cells were plated at 3*105 cells/well in fibronectin-treated (EMD Millipore) 96-well glass-bottom plates (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ml of culture was supplemented with 3 mg/ml propidium iodide (Sigma; stock 1 mg/ml in phosphate buffered saline (PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000037282 (shRNA Trim39#2) and TRCN0000438509 (shRNA Trim39#3) were from Sigma-Aldrich. Lentiviral particles were produced as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... bafilomycin A1 and 3-methyladenine were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich), an equivalent volume of DMSO was added to control samples.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The plates were washed 3 times with 0.05% Tween-20 (P2287, Sigma-Aldrich) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) containing 1 mM PMSF and phosphatase inhibitor cocktail 3 (Sigma-Aldrich). Cells were scraped off ...
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bioRxiv - Developmental Biology 2021Quote: ... The non-specific binding of antibodies was blocked with 3% BSA (Sigma-Aldrich) for 45 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... (1.5% CMC (3% (w/v) carboxymethylcellulose solution in sterile distilled water (Sigma C4888)) ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mg/ml N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (Sigma, 03450) in 50 mM HEPES were added on top of the hydrogels and incubated for 30 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... 2% DMSO and 300 μM 3-maleimidobenzoic acid N-hydroxysuccinimide ester (MBS; Sigma) dissolved in microtubule stabilization buffer specific for algae (MTB ...
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bioRxiv - Developmental Biology 2020Quote: ... 3-5 colonies were picked and incubated in 2mL Terrific Broth (T0918, Sigma) containing 100µg/mL Ampicillin for 8 hours ...
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bioRxiv - Genomics 2020Quote: ... before addition of 500 μM indole-3-acetic acid solution (“auxin”, Sigma-Aldrich) for different times to induce RPB1 degradation ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with 100 μg/ml of 3×FLAG peptide (Sigma). The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and were blocked with 3% bovine serum albumin (BSA; Sigma) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] were acquired from Sigma (USA). Purified water was prepared with a Milli-Q purification system from Millipore (Milford ...
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bioRxiv - Neuroscience 2019Quote: ... interleukin alpha (IL-1a) (3 ng/ml; Sigma-Aldrich; Cat. No. I3901-5UG) and complement component 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... selected for 2-3 weeks with 1 µg/ml of puromycin (Sigma- Aldrich), and surviving cells were fixed with methanol and stained with Giemsa (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then washed with PBS containing 3% Bovine Serum Albumin (BSA, Sigma). Following MeTAFlow assay the cells were passed through a strainer cap to achieve a single cell suspension and immediately analyzed using BD FACS Aria Fusion SORP Cell Sorter ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: ... Elution was achieved by using 250 μg/ml 3 × Flag peptides (F4799, Sigma). The resulting eluates were resuspended in TRIzol for the following RNA extraction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All fish were euthanized with ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma-Aldrich), and then fixed in ethanol or RNAlater solution (Thermo Fisher) ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... they were washed and blocked with 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) and 100 μM NiSO4 for 20 min ...
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bioRxiv - Genomics 2020Quote: ... To the XRN1-AID strain 0.2mM of auxin (3-Indoleacetic acid; Sigma-Aldrich) was added to the media 30 and 60 minutes before harvesting ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Genomics 2019Quote: ... thyroxine (0.4 µg/ml) and glucose (3 g/l) (all from Sigma-Aldrich).