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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for 6 METHOXY 2 METHYL 1 PHENYLSULFONYL 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos and larvae in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Biophysics 2023Quote: ... and 2 mM magnesium chloride) with the addition of 1 μL Benzonase (Millipore Sigma) by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... and diluted 1:2 in cell culture medium containing 10 μg/ml polybrene (Sigma) before adding to HCT-8 cells ...
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bioRxiv - Bioengineering 2023Quote: ... 2 cover glass slips coated with fibronectin (Sigma-Aldrich; 10 μg·mL−1 in DPBS) in 24-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... purified cell pellets were resuspended and incubated with 2 mg ml-1 iodoacetamide (Sigma) at 4 °C for 30min ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2 mM L-glutamine and 1% P/S (both from Sigma-Aldrich) and they were activated with 125 ng/mL (final concentration ...
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bioRxiv - Biophysics 2023Quote: ... 1 μl of a 2 mM stock solution of Gramicidin D (G5002, Sigma-Aldrich) in ethanol was added to 2 ml of external solution with 5 mM glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... as in28 and rat anti-laminin-2 1:100 233 (Sigma-Aldrich, Cat. #L0663). For macrophage staining these included ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit α-phospho [(Thr202/Tyr204, Thr185/Tyr187)-ERK1/2 (12-302, Millipore, 1:1500); rabbit α-ERK1/2 (4695 ...
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bioRxiv - Microbiology 2023Quote: ... 2 (Oxoid, Hampshire, UK) supplemented with menadione (1 mg/mL) (Sigma Aldrich, Diegem, Belgium), hemin (5 mg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... The stained kidneys were cleared using BABB (Benzyl alcohol/Benzyl benzoate, 1:2, Sigma, cat numbers 108006 and B6630 ...
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bioRxiv - Biophysics 2023Quote: ... Isolated oocytes were incubated in 2 mg mL-1 collagenase type I (Sigma-Aldrich) for 6 hours to remove the follicular membrane and incubated in frog Ringer’s solution containing 88 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) together with 2 µl of 100 mg ml−1 Nile Red (Sigma) and further incubated for 5 min at 30 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... for 2 hours at room temperature and counterstained with Hoechst (1:1000; Sigma-Aldrich) for 10 minutes prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 2 uM A-83-01 (Selleckchem) and 1-5% (v/v) FBS (Sigma-Aldrich). After 1-2 weeks ...
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bioRxiv - Biophysics 2024Quote: ... MhsT was concentrated to 1-2 mL using 50,000 Dalton MWCO filter concentrators (Millipore) and centrifuged at 105,000xg for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... medium was changed to MEF medium with Dox (2 µg ml−1, from Sigma) to induce transgene expression (marked as day 0) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were transferred to BABB solution (1:2 ratio of benzyl alcohol, Sigma-Aldrich, 305197 ...
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bioRxiv - Genomics 2024Quote: ... placed into an enzymatic dissociation cocktail (2 U ml−1 Liberase (5401127001; Sigma–Aldrich) and 2 U ml−1 elastase (LS002279 ...
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bioRxiv - Cell Biology 2024Quote: ... Zebrafish embryos were treated with 0.2 mM 1-phenyl 2-thiourea (Sigma-Aldrich, P7629) after 24 hpf to prevent pigment formation ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal anti-RdRp/nsp12 (Sigma-Aldrich, SAB3501287-100UG, 1:500, 2 µg/mL), mouse monoclonal anti-nucleocapsid (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were treated with 0,003% 1-Phenyl-2-thiourea (PTU) (Sigma-Aldrich, USA, #P7629) to prevent pigmentation of the skin and to avoid any interference on the regions of interest during acquisition ...
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bioRxiv - Genetics 2024Quote: ... sclerotome induction was initiated with 1 μM C59 and 2 μM purmorphamine (Sigma-Aldrich). After the first day of sclerotome induction (day 4 ...
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bioRxiv - Immunology 2022Quote: ... cells were transduced by adding ~1-2 ml of RV supernatants supplemented with 50 μM 2-βME and 8 μg/ml polybrene (Millipore, Cat # TR-1003-G), followed by centrifugation for 90 min at 524g at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... samples were taken up in a 1:1 (v/v) mixture of 100% MeOH and a 2:1 (v/v) mixture of benzyl alcohol (Sigma Aldrich, Saint Louis, USA) and benzyl benzoate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... FLAG-IP lysis buffer (50 mM HEPES pH 8.0, 100 mM KCl, 2 mM EDTA, 0.1% NP40, 10% glycerol, 1 mM PMSF, 1 mM DTT, and Sigma protease inhibitors P8340 1:500). Resuspended lysates were first froze and thawed twice (dry ice and 37 °C waterbath ...
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bioRxiv - Cancer Biology 2022Quote: ... After 2 hours medium was replaced with 2 mL medium containing 2 % FBS and 2 mg/mL polybrene (Sigma). After an hour this medium was removed and replaced with 2mL of medium containing 5x 106 IU of lentiviral vector ...
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bioRxiv - Neuroscience 2021Quote: Quantification of IL-6 in media was assessed via Human IL-6 ELISA kit according to manufacturer instructions (Sigma Aldrich; St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... transduced cells were selected by incubating the monolayer in DMEM:F12 (1:1)+10% FBS containing 2 μg/mL Puromycin dihydrochloride (Sigma, USA) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9-sgRNA vector and a control vector (pEGFPN1) in the ratio: 2:1:1 using PEI (18) from Sigma Aldrich, Inc ...
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bioRxiv - Developmental Biology 2021Quote: Embryos used for long-term imaging or imaging after 1 dpf were treated with 0.003% 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) to prevent melanin pigment formation.
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bioRxiv - Biochemistry 2019Quote: ... were amplified by PCR from genomic DNA and cloned into the multiple coning sites (MCS) 1 and 2 using the NcoI/NotI and NdeI/XhoI restriction sites of a modified pETDuet-1 vector (Novagen) containing an N-terminal PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... 400 mM KCl, 2 mM MgCl2, 0.002% Brij35, 1 mM β-mercaptoethanol, 1 mM imidazole, 50 mM L-Arginine, Sigma#A5006 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Cell Biology 2019Quote: ... The pellet was lysed in lysis buffer (50 mM Tris-HCl, pH 8, 125 mM NaCl, 1% NP-40, 2 mM EDTA, 1 mM PMSF [Sigma, 93482-50ML-F] ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
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bioRxiv - Cell Biology 2020Quote: ... For AsiSI or I-PpoI nuclease induced DSBs, cells were treated with Shield-1 (1 μM, AOB1848, Aobious) and 4-OHT (2 μM, H7904, Sigma) for 5 hours before washing with PBS and adding back media.
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bioRxiv - Plant Biology 2021Quote: ... 1 g of the infiltrated leaf tissue was ground in 4 mL of extraction buffer (1/2 tablet of Sigma Complete™ mini protease inhibitor (per 15 mL) ...
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bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v) microcrystalline cellulose and 2% FCS (mixed 1:1) overlay (Sigma-Aldrich). The assay was incubated at 37°C and 5% CO2 for 70 hours then fixed in 4% PFA for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2) acid solution for 8 min (#HT153, #HT152, Sigma), and then they were stained in Aniline Blue solution for 5 min (#HT154 ...
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bioRxiv - Developmental Biology 2022Quote: ... to induce brain regional patterning, EBs were treated individually with SAG (1:10,000) (Milli- pore, 566660) + IWP-2 (1:2,000) (Sigma-Aldrich, I0536) for ventral identity and with cyclopamine A (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 1 - 2 µl of solutions containing 0.1 or 1% of the non-ionic detergent Triton X-100 (TrX; Sigma-Aldrich), dissolved in 0.1 M phosphate-buffered saline (PBS ...