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Citations for Millipore Sigma :
3801 - 3850 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Parasites were maintained in fresh human erythrocytes at 2% hematocrit in complete RPMI-1640 (Sigma) medium in addition to 0.5% AlbuMaxII (Gibco by Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... was from Thermo Fisher Scientific (Waltham, MA) and human tissue plasminogen activator (tPA) from Sigma Aldrich (St. Louis, MO). Rabbit monoclonal anti-PAI-1 [EPR17796] (ab187263 ...
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bioRxiv - Cancer Biology 2023Quote: ... DMEM medium supplemented with 10% human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... human CD3+ T cells were stimulated with 10ng/ml PMA and 1μM Ionomycin (Sigma-Aldrich) or with 1 μg/mL pre-coated α-CD3 and 1 μg/mL soluble α-CD28 ...
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bioRxiv - Cell Biology 2023Quote: ... Human colorectal adenocarcinoma cells (Caco-2, ATCC) were maintained in medium consisting of DMEM (Sigma), 100 U/ml penicillin ...
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bioRxiv - Immunology 2023Quote: ... Human acute monocytic leukemia cell line (THP-1) was cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was incubated in Sudan Black (0.3% Sudan Black B (Sigma, Cat# 199664) in 70% ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA encoding human PF4 ORF was cloned into the pET-15b vector (Novagen, Madison, WI) and transformed into Origami B (DE3 ...
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bioRxiv - Immunology 2023Quote: ... diluted with a 10x volume of RPMI with 10% heat-inactivated human AB serum (Sigma), and centrifuged at 300 x g for 7 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... sub-cultured until mid-exponential phase then opsonized in human serum (Sigma-Aldrich, no. H4522) for 30 min ...
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bioRxiv - Immunology 2023Quote: Human macrophages were harvested from culture using PBS+5mM Ethylenediaminetetraacetic acid (EDTA; Sigma Aldrich #E7889) and cell lifters (Fisher Scientific #08-100-240) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Immunology 2023Quote: ... 20 mg of purified human IgG in PBS was immobilised to CNBr-activated Sepharose (Sigma) (10 mg IgG/ml packed Sepharose) ...
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bioRxiv - Microbiology 2023Quote: ... Monocytes were selected by adhering PBMCs in RPMI with 5% pooled AB human serum (Sigma), 1 mM HEPES (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 µg/mL, HPA006028, Sigma/Prestige Antibodies) was performed at 4 °C overnight in PBS/10% AB serum ...
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bioRxiv - Biochemistry 2024Quote: ... Human CXCL12 [50 ng/mL] and EGF [50 ng/mL] (Sigma Aldrich SRP3276 and SRP6253) were prepared in serum-free DMEM and used as chemoattractant in the lower chamber ...
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bioRxiv - Neuroscience 2024Quote: ... sections were double-immunolabeled for human nuclei and either Htt (1:200; clone EM48, Millipore) or IBA-1 (1:1,000 ...
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bioRxiv - Bioengineering 2024Quote: ... 10 µg/L human epidermal growth factor (hEGF; E9644, Sigma-Aldrich, St. Louis, MO, USA), 1% ITS-X supplement (094-06761 ...
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bioRxiv - Microbiology 2024Quote: ... TSB-grown bacteria were centrifuged and resuspended in human serum from male AB plasma (Sigma) and incubated for 16 h at 37 °C with shaking (180 rpm) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO; C7521). Ex Plus medium was added to both the apical and basolateral compartments and changed daily until the cells reached confluency on the inserts ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines from culture supernatants were analyzed using human cytokine MILLIPLEX Luminex (EMD Millipore). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Downregulation of PAK6 expression was performed utilizing an shRNA against human PAK6 (sh-1944, Sigma). Western blot in SH-SY5Y cell extracts was performed with anti-PAK6 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... we coated plates with 1 µg/ml of recombinant NP119 and used a goat anti-mouse IgG-HRP (ψ-chain specific) as secondary antibody (Sigma, A3673-1ML; diluted 1:1000). To measure anti-drug antibody responses directed against the AAV-delivered YTS169-IgG2a we coated plates with 1 µg/ml of YTS169-IgG2a or YTS191-IgG2a and used a goat anti-mouse IgG1-HRP (Jackson ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated with rabbit polyclonal anti-human P2X4 (Cat. No. HPA039494, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-mouse P2X4 (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... primary mouse myoblasts and human immortalized myoblasts were transfected with 60nM final of siRNAs (Sigma Aldrich) using LipoRNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with 1 ml of Stimulation Medium supplemented with 0.5 nM human insulin (Sigma) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore). Cytokines in mouse BALF were analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Neuroscience 2022Quote: Human iPSC lines were maintained on irradiated MEFs in hESCs medium [DMEM/F12 (Sigma-Aldrich, D6421) supplemented with 20% KO Serum Replacement (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... followed 48 hours later by i.p injection of human chorionic gonadotropin (hCG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human alveolar type II epithelial cell line A549 was purchased from Sigma-Aldrich (Poole, UK) and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2019Quote: ... which is made by supplementing RPMI Medium 1640 with 5 µg/mL human insulin (Sigma-Aldrich), 5 µg/mL human transferrin (Sigma-Aldrich) ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... or alkaline phosphate-labeled goat-anti-human IgM (1:2000, A9794, Sigma, St. Quentin Fallavier France). Conjugate binding was quantified with SIGMAFAST p-nitrophenyl phosphate (Sigma ...
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bioRxiv - Immunology 2019Quote: ... they were intraperitoneally injected with 2.5 iu human chorionic gonadotropin (HCG, Sigma Aldrich, St. Louis, MO) and immediately mated with male mice overnight (16 hours) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated on glass coverslips coated with 50µg/mL human type IV collagen (Sigma C7521) 24 to 48h before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... META-enriched cultures were isolated via resistance to complement-mediated lysis in 20% human serum (Sigma) for 30min at 37°C and validated via sherp expression [21,23].
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bioRxiv - Immunology 2019Quote: Human and mouse omentum samples were fixed for one hour on ice in 10% NBF (Sigma) and then permeabilized in PBS 1% Triton-X 100 (Sigma ...
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bioRxiv - Genomics 2021Quote: DNA from human keratinocyte cells was extracted using the “GenElute-Mammalian Genomic DNA Miniprep Kit” (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then 50 μL of alkaline phosphatase-conjugated secondary goat anti-Human anti-IgG (Sigma Cat # A9544), anti-IgA (Abcam Cat # AB97212) ...
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bioRxiv - Bioengineering 2021Quote: Immortalized human neural progenitor cells derived from the ventral midbrain (ReNCell VM) were obtained from Millipore. Cells were expanded on laminin-coated tissue culture flasks ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Cell Biology 2021Quote: ... Cytokines: interferon gamma (IFNγ) (SRP3058) and human Interleukin-4 (IL-4) (I4269) were from Sigma-Aldrich, UK ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (A1653) and mouse serum albumin (A3139) were purchased from Sigma (St Louis, MO). Fluorescently labeled human serum albumin (HS1-S5-1 ...
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bioRxiv - Neuroscience 2020Quote: ... were co-transfected into U87 human primary glioblastma cells by using ESCORT V transfection reagent (Sigma). The fold increase in relative luciferase activity is a product of the luciferase activity induced by TCF7l2-S2 or TCF7l2-E2 (backbone vector pCMV ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were washed and incubated with HRP-conjugated anti-human IgG antibody (Sigma-Aldrich; 1:10.000) followed by chemiluminescence detection.
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bioRxiv - Cell Biology 2021Quote: ... HAP1 cells were transfected with 600 ng/ml human VRK2 MISSION® esiRNA (EHU070731, Sigma-Aldrich) using Lipofectamine RNAiMAX (13778075 ...
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bioRxiv - Cell Biology 2021Quote: ... Fibronectin-coated coverslips were prepared by first covering them with 100 µL of human (Sigma F0556) or bovine (Sigma F01141 ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were cultured at 4% haematocrit (using human O+ RBCs) and synchronised with 5% sorbitol (Sigma). For transfection ...
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bioRxiv - Systems Biology 2021Quote: Dispersed human islets and iPSC-beta cells were exposed to 0.5 mmol/L palmitate (Sigma-Aldrich) for 24 h and 48h ...