Labshake search
Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for 6 Chloro N methyl pyrimidine 4 5 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
-
bioRxiv - Cancer Biology 2020Quote: PKO cells transfected with Flag-tagged OGT before cultured in 5 mM glucose were collected and incubated overnight at 4°C with ANTI-FLAG M2 affinity gel (Sigma, A2220). Immunoprecipitated complexes were eluted and stained with Coomassie blue ...
-
bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were cultured for one week in 4 ml YNB media with a final concentration of 5 mM Nicotinamide (Sigma I17451). For copper treatment ...
-
bioRxiv - Cancer Biology 2021Quote: ... alongside a topical treatment for the tail skin (tails were dipped for 5 seconds in 25 mg/ml 4-hydroxytamoxifen (Sigma H6278) in DMSO) ...
-
bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
-
bioRxiv - Cell Biology 2022Quote: ... followed immediately by an equal volume of a freshly-prepared 5 mg/mL solution of 4-hydroxy-α-cyano-cinnamic acid (Sigma) in 50% aqueous (v:v ...
-
bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... The fused embryos were treated with 5 μmol/L ionomycin for 4 min and then incubated in mSOF medium (Sigma, USA) containing 2 mmol/L 6-Dimethylaminopurine(6-DMAP ...
-
bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
-
bioRxiv - Bioengineering 2022Quote: ... hBMSCs were frozen at passage 4 with 5*106 cells/ml in freezing medium containing fetal bovine serum (FBS, BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
-
bioRxiv - Neuroscience 2024Quote: Mice aged 3 to 5 months were deeply anesthetized and transcardially perfused with 50mL PBS followed by 50mL 4% (w/v) paraformaldehyde (Sigma-Aldrich) in PBS ...
-
bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... DHEA sulfate (5-androsten-3β-ol-17-one-3-sulfate) and testosterone (4-androsten-17β-ol-3-one) were purchased from Sigma Aldrich. 11β-hydroxyandrostenedione (11β-hydroxy-4-androstene-3,17-dione) ...
-
bioRxiv - Pathology 2023Quote: ... Freshly resected left ventricular tissue (∼2.5-4 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 µg/mL, Sigma, 47036) 30 min at 40C in BIOPS buffer containing the following ...
-
bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: ... Half of the beads were then incubated for 1 h at 4°C in 50 µl of 5 mM free CoA (C4282, Sigma-Aldrich) (dissolved in H2O) ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
-
bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
-
bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich] ...
-
bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
-
bioRxiv - Immunology 2023Quote: ... Slides were washed in 1X PBS for 5 min and coverslipped with mounting media (50% glycerol – Sigma, G5516 and 4% propyl gallate – Sigma, 2370).
-
bioRxiv - Microbiology 2023Quote: HLCs were infected at day 18 of the differentiation protocol at an MOI of 5 infectious units (IU) in the presence of 4% polyethylene glycol 8000 (PEG, Sigma-Aldrich) and 1.5% DMSO (Sigma-Aldrich) ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were exposed for six hours to virus with an MOI (multiplicity of infection) of 5 viral particles/cell in the presence of 4 μg/mL polybrene (Sigma-Aldrich). Cells were then washed ...
-
bioRxiv - Cell Biology 2024Quote: ... Parasites were synchronized to a 4-hour window by magnet-purifying schizonts and then incubated with uninfected erythrocytes for 4 hours before treatment with 5% D-sorbitol (Sigma S7900).
-
bioRxiv - Neuroscience 2024Quote: ... The medium was changed every 3–4 d and enriched with granulocyte-macrophage colony-stimulating factor (5 ng/ml GM-CSF, Sigma-Aldrich). After approximately 14 d at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: The femurs of 10-week-old mice were analyzed by VivaCT 2 weeks before (baseline) and 4 weeks after a single intraperitoneal injection (i.p.) of 5 mg/kg doxorubicin (Sigma-Aldrich, MO) formulated in H2O at 1 mg/ml or vehicle ...
-
bioRxiv - Biochemistry 2024Quote: ... Actin was then cleaved off the beads overnight at 4 °C using 5 μg/ml TLCK treated chymotrypsin (SIGMA: C3142-25MG) which was then inactivated by 1mM PMSF ...
-
bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
-
bioRxiv - Microbiology 2024Quote: ... placed in digestion media containing 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma) ...
-
bioRxiv - Genetics 2024Quote: ... Laemmli Buffer (100 mM Tris pH 6.8, 20% glycerol, and 4% SDS (Carl Roth, Germany)) and 5% β-mercaptoethanol (Sigma-Aldrich) were added to the samples after which they were heated to 95°C for 10 minutes.
-
bioRxiv - Cell Biology 2019Quote: ... the slides with attached intact axonemes were treated overnight at 4°C with blocking buffer containing the diluted primary antibody (mouse anti-acetylated tubulin, Sigma-Aldrich, clone 6-11B-1, 1:1000). On the next day ...
-
bioRxiv - Physiology 2021Quote: ... The resulting CX3CR1CreERT2(+/−)::Ptger4fl/fl offspring were injected at 6 weeks of age with 2 doses of tamoxifen (T5648, Sigma, 4 mg s.c., prepared in corn oil) 48 hours apart ...
-
bioRxiv - Cell Biology 2021Quote: ... 150 μM 4-(4-Morpholinobutylthio)phenol (MoTP) (Sigma) was added to embryos kept at 28.5°C from 24hpf onwards ...
-
bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
-
bioRxiv - Neuroscience 2020Quote: ... 4-AP (4-Aminopyridine, 500 μM, Sigma-Aldrich), FK-506 (1 μM ...
-
bioRxiv - Bioengineering 2019Quote: ... (ii) 4 hours of 4% sodium deoxycholate (Sigma) at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) was purchased from Sigma and dissolved in ethanol ...
-
bioRxiv - Developmental Biology 2021Quote: ... 4-hydroxy tamoxifen (4-OHT, Sigma-Aldrich, H6278) treatment (1 µM in EtOH ...
-
bioRxiv - Immunology 2021Quote: 4-hydroxytamoxifen (Tx) (4-OHT, #T5648, Sigma-Aldrich) was dissolved in sunflower oil to a concentration of 10 mg/mL for i.p ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μM 4-hydroxy tamoxifen (4-HT; Sigma) was added at the time of activation.
-
bioRxiv - Neuroscience 2022Quote: (Z)-4-Hydroxytamoxifen (4-OHT; Sigma-Aldrich #H7904) was first prepared as 25 mg/mL stock solutions in ethanol and frozen at −20C ...
-
bioRxiv - Neuroscience 2024Quote: ... and 4-aminopyridine (4-AP, 100 μM, Sigma), mecamylamine (10 μM ...
-
bioRxiv - Neuroscience 2022Quote: ... Chemicals used: 4-Aminopyridine (4-AP, Sigma #A78403), tetrodotoxin (TTX ...
-
bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT, Cat#: H7904, Sigma-Aldrich) was dissolved at 10mg/ml in peanut oil and 1mg was administered by intraperitoneal injection from E10.5 for 4 consecutive days ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
-
bioRxiv - Biochemistry 2023Quote: ... 4 mM MgCl2 and 4% DMSO (Sigma, 276855). The mixture was incubated for 30 min at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... 3-ethoxy-4-hydroxybenzaldehyde (121-32-4, Sigma), N-(acid-PEG10)-N-bis(PEG10-azide ...
-
bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OH TAM, Sigma-Aldrich, H7904), L-selenocystine (Sigma-Aldrich ...