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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for Recombinant Human CFHR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein solutions were concentrated using Amicon Ultra centrifugal filter (Millipore) for application to FPLC AKTA pure (GE Heathcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; prewashed in lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was transferred to an Immobilon PVDF membranes (Millipore Sigma) via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Merck-Millipore) and washed twice in TBS buffer followed by incubation in Intercept-TBS Blocking Buffer (Li-COR ...
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bioRxiv - Cell Biology 2024Quote: ... Relative protein loading was visualized using Ponceau S Solution (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... then after preclearing with protein A–Sepharose beads (Sigma-Aldrich), HY5 antibody (AS12 1867 ...
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bioRxiv - Microbiology 2024Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was induced using 0.5 µg/ml doxycycline (Sigma) for 24 h.
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently with protein A agarose beads (16-125, Millipore) for another 4 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples (50 µg) were reduced with DTT (Millipore Sigma) at a final concentration of 20 mM at 95°C for 10 minutes and alkylated with iodoacetamide (Millipore Sigma ...
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bioRxiv - Genetics 2024Quote: ... Protein bands were visualized using enhanced chemiluminescence reagents (Millipore, WBKLS0500).
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bioRxiv - Immunology 2024Quote: ... wells were coated with Protein A from Staphylococcus aureus (Sigma). After 48h ...
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bioRxiv - Cancer Biology 2024Quote: ... was pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was measured using Bradford Reagent (B6916, Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were blotted on a PVDF membrane (Merck Millipore, IPVH00010). Membranes were incubated in primary antibodies mouse anti-Vinculin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Secreted proteins were reduced in 10mM Dithiothreitol (DTT; Sigma-Aldrich) at 20°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich, USA) on a spectrophotometer (Infinite F50 Tecan ...
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bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to immobilon NC transfer membranes (HATF00010, Millipore) at 400 mA for 2 h in transfer buffer (25 mM Tris ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified from the medium (Ex-Cell405, Sigma-Aldrich) 2 days post-infection by directly flowing over Ni-Penta™ Agarose-Base Resin (Marvelgent Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was concentrated using a centrifugal filter (Millipore; UFC803024) in storage buffer (20 mM Tris HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin in TBST (20 mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein samples were transferred to a Immobilon NC membrane (Millipore) using a Genie Blotter (Idea Scientific) ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration was measured using the BCA Assay (Sigma).
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bioRxiv - Neuroscience 2022Quote: Protein concentrations were determined using the Bradford method (B6916, Sigma), samples were boiled for 5 min in 4x-Laemmli’s sample buffer (1610747 ...
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bioRxiv - Developmental Biology 2022Quote: ... rabbit anti-pro-surfactant protein C (Sigma, Ab3786; 1:2000), rabbit anti-TGFβ2 (Proteintech ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a PVDF-FL membrane (05317, Millipore) with a current of 2.5 mA/cm2 for 1 h ...
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bioRxiv - Microbiology 2023Quote: Total protein concentration was determined colorimetrically (Bradford Reagent, Sigma-Aldrich). The standard curve was prepared using Bovine Serum Albumin (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... protein expression was induced with 0.5 mM IPTG (Sigma-Aldrich) for 1.5 h ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... Proteins in gels were stained with SYPRO orange (Sigma-Aldrich), and gels were imaged with a Typhoon FLA9500 scanner (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred at 100 V to PVDF membrane (Millipore) for 1.5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 40 μl of prewashed protein A beads per sample (Sigma; equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich B6916). Lysates were resolved on a 4-12% Bis-Tris NuPage gel (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in 2× Laemmli buffer (Sigma-Aldrich), boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 10 mg/ml reduced glutathione (Sigma) in 50mM Tris pH 8 ...
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bioRxiv - Neuroscience 2023Quote: ... immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799). Trem2 was immunoprecipitated with either a Rabbit monoclonal antibody raised against the carboxy-terminus of mouse Trem2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (EMD Millipore, Darmstadt, Germany) and the membrane was blocked with 5% fat-free milk powder in TBST and incubated for 1 h at room temperature or overnight at 4°C with primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured using BCA assay (Sigma-Aldrich #1003290033). 40–50 μg of cell lysate were loaded in precast SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis ...
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bioRxiv - Physiology 2023Quote: ... Separated proteins were transferred to polyvinylidene difluoride membrane (PVDF, Millipore) using a Trans-Blot Turbo Transfer Pack (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Activated protein was transferred to a PVDF membrane (ED Millipore) using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein loading was visualized with Amido Black Staining Solution (Sigma).
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred onto PVDF membrane (Immobilon-FL, Millipore) using a semi-dry transfer apparatus (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Filtered media was then loaded onto a Protein A (Millipore) column prewashed in ice cold HEPES-buffered saline (HBS ...
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bioRxiv - Cancer Biology 2023Quote: Protein was isolated using RIPA buffer with protease (Sigma Aldrich) and phosphatase cocktail inhibitors (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). Membranes were blocked in 5% skim milk (Nacalai ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was determined using Bradford reagent (Sigma Aldrich, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were precipitated by adding 5-sulfosalicylic acid (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein expression was induced by adding L-arabinose (Sigma-Aldrich) to a final concentration of 0.2% when the cultures reached a OD600 of 0.4 ...