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Citations for Millipore Sigma :
3701 - 3750 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The sperm suspension was capacitated for 1 h in 200 μl HTF medium (Millipore, MR-070-D). Subsequently ...
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bioRxiv - Genetics 2024Quote: Cell lysates were harvested from 100,000 cells using 30µl of TBS + 1% Octyl β-D-glucopyranoside (Sigma) and mini-cOmplete protease inhibitor table (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Neurons were plated on coverslips coated with poly-D-lysin (0.1 mg/mL; Sigma or Thermo Fisher) at a density of 180,000 cells/well ...
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bioRxiv - Biophysics 2024Quote: ... 0.0005% CHS) then protein was eluted with elution buffer (wash buffer with 10 mM d-Desthiobiotin (Sigma)) ...
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bioRxiv - Systems Biology 2020Quote: All channels were coated with 1 mg/mL poly-D-lysine (PDL) solution (Sigma-Aldrich, St. Louis, MO) to prevent the detachment of collagen gels from the channel walls [43] ...
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bioRxiv - Cell Biology 2020Quote: ... 1□mM MgCl2, 0.2□mM Na2HPO4, 12□mM NaHCO3, 5.5□mM D-glucose, 1.8□mM CaCl2; Sigma–Aldrich) at physiological conditions (37°C ...
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bioRxiv - Genetics 2021Quote: Induced mature brown adipocytes upon Fto knockdown were treated with 5 mg/mL actinomycin D (A9415, Sigma-Aldrich) to assess RNA stability of Ucp1 and C/ebpα transcripts ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM MgCl2, 0.2 mM Na2HPO4, 12 mM NaHCO3, 5.5 mM d-glucose, and 1.8 mM CaCl2; Sigma-Aldrich) for at least 30min at 37°C and then imaged on a Zeiss Axio Observer Z1 inverted microscope equipped with a Hamamatsu ORCA-Flash 4.0 sCMOS camera ...
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bioRxiv - Biophysics 2022Quote: ... we plated the cells on the 12 mm round coverslips coated with 1x Poly-D-Ornithine (Sigma-Aldrich) in 12 well plate ...
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bioRxiv - Bioengineering 2022Quote: ... and antimycin A (AA) (500 nM) and 2-deoxy-D-glucose (2-DG) (25 mM, all Sigma-Aldrich) were prepared in XF assay medium and loaded into the appropriate injection ports on the cartridge plate and incubated for ten minutes in a non-CO2 incubator at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Single colonies were inoculated and protein expression was induced using isopropyl-β-D-thiogalacto-pyranoside (IPTG, Sigma Aldrich). Cell lysates were screened for protein expression using SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Genetics 2020Quote: ... The cumulus-free oocytes were washed and kept in the KSOM+AA medium (Millipore, Cat#MR-121-D) in an incubator (Sanyo ...
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bioRxiv - Biophysics 2021Quote: ... 2 x 105 cells per 60 mm culture dish were seeded on poly-D-ornithine (Sigma Aldrich, P8638) coated #1.5 18 mm glass coverslips (Marienfeld ...
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bioRxiv - Bioengineering 2021Quote: The excised gel fragment was electroeluted in 1X TAE buffer using 3.5 kDa MWCO D-Tube dialyzers (Novagen) according to the manufacturer′s protocol with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Undifferentiated cells were removed by adding Ara-C (5 μM final concentration; cytosine β-D-arabinofuranoside hydrochloride, Sigma) at day 4 post-induction (4 dpi) ...
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bioRxiv - Neuroscience 2021Quote: ... Glial cells proliferation was inhibited by adding Ara-C (cytosine β-D-arabinofuranoside hydrochloride, 2.5 μM, Sigma-Aldrich) at DIV6-8 ...
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bioRxiv - Cancer Biology 2020Quote: ... poly-D-lysine hydrobromide and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (Saint-Quentin Fallavier, France). Biotech grade Tris was obtained from Interchim (Montlucon ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were cultured from E18 Igsf8 conditional knock out mice and plated on poly-D-lysine (Millipore) and laminin (Invitrogen)-coated glass coverslips (Glaswarenfabrik Karl Hecht) ...
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bioRxiv - Microbiology 2020Quote: ... Tween-20 binding buffer (0.05 M Tris pH 7.4, 0.15 M sodium chloride, 0.2% n-Dodecyl β-D-maltoside; all reagents from Sigma), which were then washed twice with 200 µL of PBST binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... and the IPTG-inducible promoter (Plac) was activated with 1 mM IPTG (β-D-1-thiogalactopyranoside, Sigma-Aldrich). For all related experiments ...
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bioRxiv - Bioengineering 2021Quote: ... Bone marrow cells were cultured (7 d, 37°C, 5% CO2) in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma) enriched with 10% L929-conditioned media (containing macrophage colony-stimulating factor) ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal) (Sigma) at a final concentration of 100 μg/ml as indicators ...
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bioRxiv - Cell Biology 2020Quote: ... and plated on coverslips or tissue culture plastic coated with 1 mg/ml poly-D-lysine (Sigma-Aldrich). For axonal length ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by 1 mM isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, Cat. No. I6758) and occurred overnight at room temperature under shaking ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Biophysics 2020Quote: ... Both 2N4R and Tau187 variant expressions were induced by incubation with 1 mM isopropylß-D-thiogalactoside (Sigma Aldrich) for 2–3 h ...
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bioRxiv - Bioengineering 2022Quote: ... pSANG10-Avi clones were expressed in the same way in the presence of 50μM free D-biotin (Sigma).
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bioRxiv - Neuroscience 2022Quote: Dissociated spheroids or NGN2-induced neurons were plated as a 10μl droplet in Poly- D-Lysine (Sigma-Aldrich) coated wells of a CytoView MEA 48-well plate (Axion BioSystems ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... spleens from female Balb/cJ mice were dissociated in 2 mg/mL Collagenase D (Sigma-Aldrich, Cat# 11088882001) in Opti-MEM media (Gibco ...
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bioRxiv - Neuroscience 2022Quote: Rat embryonic hippocampal neurons were grown on coverslips coated with 0.1M borate buffer (50mM boric acid, 12.5mM sodium tetraborate in water, pH 8.5) 60ug/mL Poly-D-Lysine (Sigma), and 2.5ug/mL Laminin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Lesions were assessed 14–16 d after 6-OHDA injection by challenge with apomorphine (0.05 mg/kg, s.c.; Sigma) (Schwarting and Huston ...
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bioRxiv - Neuroscience 2020Quote: ... on 75-cm2 tissue culture flasks coated with poly-D-lysine (100 μg/mL, Sigma Aldrich, cat#P6407) and incubated at 37 °C in 5% CO2 atmosphere ...
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bioRxiv - Neuroscience 2022Quote: ... dishes and plates had been applied overnight at 37°C and contained 60μg/ml poly-D-lysine (Sigma) and 2.5μg/ml laminin (Sigma ...
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bioRxiv - Genetics 2022Quote: ... Yeast cells were cultured in synthetic complete (SC) medium (2% D-galactose, 0.67% yeast nitrogen base without amino acids, 0.2% drop out (Sigma), (0.0076% uracil ...
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bioRxiv - Genetics 2022Quote: ... with 100µL of buffer solution (pH 4.3) containing 0.5 mM of 4-methylumbelliferyl-β-D-galactopyranoside substrate (Sigma). A kinetic analysis was performed at 37°C for 1-2 hours ...
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bioRxiv - Immunology 2022Quote: ... was added to a suspension of 5-nitroso-6-(D-ribitylamino)uracil (20.4 mg, 0.069 mmol) in sterile water (1.5 mL, Sigma-Aldrich). The mixture was briefly sonicated until the nitrosouracil dissolved and a colourless solution was obtained59.
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bioRxiv - Biophysics 2022Quote: ... The protein was dialysed overnight at 4°C in ITC buffer (25 mM HEPES pH 7.0 and 20 mM NaCl) using D-tube Dialysis Midi MWCO 3.5 kDa (Novagen) dialysis tubes before the experiment ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure D-2959) photoinitiator (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... GBS bearing the pFfluc-Erm plasmid must be exposed to the cofactor D-luciferin (Millipore Sigma cat. # L9504), which was prepared in a filter-sterilized stock solution at a concentration of 28 mg/mL in water ...
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bioRxiv - Biophysics 2020Quote: ... followed by overexpression after induction with 1 mM isopropyl β-D-1-thiogalactopyranoside (Sigma-Aldrich, Zwijndrecht, The Netherlands). The ClyA monomers were purified using Ni+-NTA affinity chromatography and oligomerized by incubation in 0.2 % D-maltoside n-dodecyl-β-D-maltopyranoside (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were replated on day 6 with medium containing Cytosine β-D-arabinofuranoside hydrochloride (2.5 μM, Sigma). The cells were harvested and frozen on day 10.
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bioRxiv - Immunology 2021Quote: ... which was detected using a fluorometric β-galactosidase substrate 4-methyl-umbelliferyl-β-D-galactopyranoside (“MUG”; M1633, Sigma).
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bioRxiv - Immunology 2021Quote: ... Lymphocyte digest media was then added to the lungs (1% FBS, 2.25 mg/mL Collagenase D (11088866001, Sigma), 0.15 mg/mL DNase I in PBS in 4 mL final volume) ...
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bioRxiv - Plant Biology 2020Quote: ... Pip and NHP were quantified against external standard curves of (D,L-piperidine-2-carboxylic acid, Sigma-Aldrich) and NHP with six calibration points ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Systems Biology 2020Quote: ... Syringes connected to inlets I22 and I11 also contained 1 mM isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Sigma).
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bioRxiv - Neuroscience 2021Quote: ... The cells plated for experiments on poly-D-lysine coated plates (10 µg/ml in water, Sigma-Aldrich) in Neurobasal media supplemented with 2% B27 ...
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bioRxiv - Biochemistry 2021Quote: ... Full deuteration controls were obtained by incubating SecA in buffer F containing 6M Urea-d4 (98% D, Sigma) overnight at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded on glass coverslips coated with 50 µg/ml poly-D-lysine (Sigma, cat. no. P7280). After 24 h ...