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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... washed in water until the stain turned blue approximately 2 mins and then counter stained with 1% Eosin (Sigma-Aldrich, Cat. no. 17372-87-1) for 30 Sec ...
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bioRxiv - Immunology 2022Quote: ... CHO CoV-2-Spike cells were used as target cells and were fluorescently labeled with PKH67 Fluorescent Cell Linker Kit (Sigma Aldrich) prior to incubation with mAbs ...
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bioRxiv - Microbiology 2021Quote: ... DNA was then extracted from 2 mL of cell culture using a GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich, USA). DNA samples were subjected to an initial quality check using a Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: Cell viability and cytotoxicity of antibiotics and peptides were determined against human corneal epithelial cells (HCE-2, CRL-11135, ATCC, Manassas, Virginia, USA) using cell-counting-kit-8 (CCK-8) assay (Sigma Aldrich, Merck Life Science UK Limited ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was removed and the cell pellet was resuspended in 500 μL Diluent C containing 2 μL PKH67 dye (PKH67 Green Fluorescent Cell Linker Kit, Sigma-Aldrich), incubated for 5 minutes at room temperature ...
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bioRxiv - Genomics 2019Quote: ... with 2 mL of ice-cold EZ-Prep lysis buffer from the Nuclei EZ-prep Isolation Kit (Sigma, Cat #NUC-101). The tissue was dounced 25 times with Pestle A ...
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bioRxiv - Immunology 2022Quote: ... 100-150 mg of snap-frozen tissue were mechanically homogenized in 750 µL of Lysis Solution/2-mercaptoethanol mixture and total RNA was isolated using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). Reverse transcription for up to 2 µg of total RNA was performed using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Following primary antibody incubation coverslips were washed 2× in PBS and then the proximity ligation assay was carried out (Duolink In Situ Red Starter kit (Sigma Aldrich) according to the manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA was extracted from 2 ml of overnight culture from each time point using the GenElute™ Bacterial Genomic DNA kit (Sigma) and 10 ng analysed by qPCR using a Light Cycler 480 real-time PCR system (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... in FibroGRO™ Complete Media Kit supplemented with bFGF (75 ng/mL) and 2% of foetal bovine serum (FBS, Sigma-Aldrich). Only iPSC-cFbs with a passage number ≤ 10 (P10 ...
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bioRxiv - Immunology 2023Quote: Inflammation-associated with neutrophils and monocytes tissue infiltration was indirectly determined by measuring the MPO activity in 2 cm length fragments of distal colon using an MPO colorimetric activity kit assay (Sigma-Aldrich), following the manufactures instructions.
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bioRxiv - Microbiology 2024Quote: Cytotoxicity was studied according to the manufacturer’s instruction either using cytotoxicity LDH assay kit-WST (Dojindo) or by measuring transepithelial electrical resistance (TEER) (Millicell® ERS-2, Millipore) as previously described (25) ...
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bioRxiv - Cell Biology 2019Quote: ... Other antibodies used in the study were: Rabbit anti-twinfilin-2 antibody (Sigma-Aldrich #HPA053874, WB, 1:100), rabbit anti-CAPZß antibody (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... 2 mM EDTA and 1% Triton X-100) with 20 mg/mL lysozyme from chicken egg (Sigma, L4919) using a tissue grinder (BMS BC-G10 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos older than 24hpf were transferred into E3 medium containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma P7629) to inhibit pigment formation and aid imaging.
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Sigma) (sigma) ...
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bioRxiv - Immunology 2022Quote: ... All wells were treated for 1 hour with 10 µM 2’,7’-dichlorofluorescin diacetate (DCF-DA, Sigma, D6883) in Ringer buffer (155 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...
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bioRxiv - Immunology 2019Quote: ... Manually processed tonsils and peripheral blood samples were diluted 1:2 with Hank’s Balance Salt Solution (Sigma-Aldrich) and layered over 20 mL of Histopaque-1077 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: Cortices were dissected from CTL and N4-1/2 bDKO mice with tools cleaned with 70 % ethanol (Sigma) and RNA-Zap (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... Animals were grown from 10 hours post fertilization (hpf) in 200 μM of 1-phenyl-2-thiourea (Sigma) to prevent melanogenesis (Karlsson et al. ...
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bioRxiv - Molecular Biology 2019Quote: Percoll gradients (1-2 per sample depending on final OD) were formed by vortexing 1.16ml Percoll (Sigma P1644) with 42 μl 5 M NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Tilapia of both sexes were anesthetized with 2-phenoxyethanol (1 ml/ liter; Sigma-Aldrich Corp., St. Louis, MO) and decapitated ...
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bioRxiv - Evolutionary Biology 2020Quote: Adult male frogs (n = 2) were anesthetized with 1% MS-222 (tricaine, Sigma-Aldrich, St. Louis, MO, USA) and sacrificed by decapitation ...
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bioRxiv - Cell Biology 2021Quote: ... topped up to 140 ug/ul for the final 2 h and then DNA was extracted with 20 ml Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma), precipitated with ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2020Quote: ... HIV-GFP produced with p8.91 or p8.2 used in Figure 1 were DNase treated for 2 hours at 37°C (DNaseI, Sigma) prior to ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Neuroscience 2020Quote: ... A borosilicated glass micropipette was filled with NES and 2 mM Glutamate or 1 mM GABA (Sigma Aldrich), and moved via a micromanipulator MP-225 (Sutter Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
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bioRxiv - Cell Biology 2021Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW: 114,17; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cell Biology 2020Quote: ... All embryos were maintained in Ringer’s solution at 28.5°C and treated with 0.003% 1-phenyl-2-thiourea (PTU) (Sigma-Aldrich) from 8 hpf.
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543 ...
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bioRxiv - Biochemistry 2022Quote: ... Fatty acids were extracted using a chloroform-methanol mix (2:1; Thermo-Fisher) in 0.01% butylated hydroxytoluene (Sigma) prior to centrifugation at 200 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: A375 cells in 100 mm dishes were challenged by 2 mM of octanoate-1-13C (Sigma Aldrich #296457) for 6 or 20 hrs ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Cell Biology 2022Quote: Evaporated lipids were resuspended in 10µL 2:1 chloroform/methanol and spotted on dehydrated silica plates (EMD Millipore; dehydrated by baking for 30 minutes at 100°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immobilized larvae were then immersed in MS-222 (0.02%) and 1-phenyl-2-thiourea (PTU, 0.003%, Sigma-Aldrich) containing E3 during imaging ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K). Due to the linear nature of DNA ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Neuroscience 2022Quote: ... samples were stained with the fluorescent nucleic acid marker 4,6-diamino-2-fenilindol (DAPI 1:3000; Sigma-Aldrich), and mounted with a Fluorescent Mounting Medium (DAKO ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 2:1 ratio of beads to reaction volume with an additional 1.8x supplementation of isopropanol (Sigma).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Developmental Biology 2019Quote: ... either 1 μl of rabbit α-Bru1 (gift from A. Ephrussi) or 2 μg of rabbit IgG (Millipore) were incubated with ovarian lysate over night at 4°C ...