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Citations for Millipore Sigma :
3701 - 3750 of 5569 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the eluted proteins were concentrated in an Amicon®Ultra-15 Centrifugal Filter Unit (Millipore; 10 kDa cutoff) and incubated with the SUMO protease Ulp1 (Ulp1:His-SUMO-tH2 = 1:500 w/w ...
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bioRxiv - Microbiology 2023Quote: ... and concentrated 20 times using Amicon Ultra-0.5 Centrifugal Filter Unit Ultracel 100 kDa cut-off (Sigma-Aldrich). Following the concentration process ...
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bioRxiv - Immunology 2023Quote: ... the supernatant was filtered twice with the cap of a 0.45 mm sterilized filter (Millipore, Billerica, MA, USA). The filtrate containing H ...
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bioRxiv - Microbiology 2023Quote: ... the eluate was sufficiently pure and concentrated using an Amicron Ultra-15 centrifugal filter (3 kDa MWCO, Millipore) to a final concentration of ∼14 g/L ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80°C ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Systems Biology 2023Quote: ... The supernatant (the medium) was then concentrated using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma, UFC901024) with a 10 KDa cutoff by swinging bucket centrifugation at 4,000 x g for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and 8% D2O using a Millipore Amicon Ultra-15 Centrifugal Filter Ultracel-3K (Millipore #UFC900324, 3 kDa cutoff), concentrated to a concentration of 50 μM and transferred to an NMR tube after removal of any precipitates by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The final CAS at a concentration of 0.1 cigarette equivalents mL-1 was sterilized by filtration through a 0.22 μm pore size sterile filter (Millipore) and stored at 4 °C for use in subsequent experiments.
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bioRxiv - Microbiology 2023Quote: ... at a final concentration of 0.1 cigarette equivalents mL-1 was sterilized through a 0.22 μm pore size sterile filter (Millipore) and stored at 4 °C for use in subsequent experiments.
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was collected after centrifugation at 15,000g and filtration through a 0.45 μm Millex HV filter (Millipore). AtMCRS1-GFP was purified with anti-GFP antibody-conjugated magnetic beads (Miltenyi Biotec ...
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bioRxiv - Biophysics 2023Quote: ... Fractions corresponding to monomer were con-centrated using Amicon Ultra-4 centrifugal filters (MWCO 10 kDa; Millipore Sigma). The final protein concentration of Tau187 was determined by UV-Vis absorption at 274 nm using an extinction coefficient of 2.8 cm−1mM −1 calcu-lated from absorption of Tyrosine ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... 30 μl of the mating mix was added to sterile nitrocellulose filters (0.45 μm pore size; MF Millipore) on LB plates with inducers for gene expression when necessary ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were isolated from used leukocyte filters (Bloodworks Northwest) over a Ficoll gradient (Millipore Sigma, GE17-1440-02), cryofrozen at a concentration of 10-20E6 cells/mL in 90% FBS/10% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... for 1−4 hours at 37 °C and supernatant was collected using an Ultrafree filter column (Millipore Sigma). tRNA was recovered by isopropanol precipitation.
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bioRxiv - Cell Biology 2023Quote: ... followed by concentrating via centrifuge at 14,000g at room temperature in a 30-kDa-cutoff filter (UFC503096, Millipore) to 50 μl of solution ...
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bioRxiv - Biochemistry 2022Quote: ... The final supernatant was concentrated to > 1 mg/ml using a 3 kDa centrifugal filter unit (Millipore Sigma) and frozen in aliquots stored at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Purity was assessed using SDS-PAGE and fractions were concentrated using Amicon 3500 MWCO centrifugal filters (Sigma-Aldrich).
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bioRxiv - Biochemistry 2022Quote: Whole FBS (15 mL) was passed through an Amicon Ultra 3 kDa MW cutoff centrifugal filter (#UFC900308, Millipore) and the resulting ∼15 mL that passed through the filter (corresponding to <3 kDa FBS material ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed twice with 10 mL of filter-sterilized 1X PBS (#P5368-10 PAK, Sigma-Aldrich) containing 0.3 mM CaCl2 (#C1016 ...
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bioRxiv - Cell Biology 2023Quote: ... and the lysates were transferred to the Microcon YM-30 centrifugal filter units (EMD Millipore Corporation, Billerica, MA). The lysis buffer was replaced with 200ul UA (8M Urea ...
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated using an Amicon Ultra-15 100-kDa cut-off centrifugal filter unit (Merck Millipore) before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Developmental Biology 2022Quote: ... Filtered medium was concentrated using Amicon Ultra-15 Centrifugal Filter Unit (3 kDa cut-off; Millipore, cat. # UFC900308) at 5,000 g for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM EDTA and 1 mM dithiothreitol (DTT)] using a Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma). The sample was treated with Precission protease to remove the His-tag and create an authentic Mpro C-terminus ...
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bioRxiv - Biochemistry 2023Quote: ... The collected flowthrough was pooled and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S75 (16/600 ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Microbiology 2022Quote: ... Viral stocks for in vivo studies were concentrated using Amicon ultra-centrifugal filters (100 kDa MW-cutoff, Millipore). All in vivo infections were carried out in a CDC/USDA-approved BSL-3 facility at ISMMS CCMS.
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bioRxiv - Microbiology 2023Quote: ... Proteins were filter-concentrated using centrifugation and a 10-kDa or 30-kDa cut-off column (Millipore Sigma) and dialyzed at 4 °C for ∼24 h against Dialysis Buffer (50 mM Tris-HCl pH 7.5 ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... The dialyzed protein was concentrated using an Amicon Ultra centrifugal filter (10 kDa molecular weight cutoff) (Millipore Sigma). The protein concentration was determined using the BCA Protein Assay Kit (Pierce).
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bioRxiv - Biochemistry 2023Quote: ... The eluted samples were concentrated to 500 μL volumes using Amicon Ultra filter units (MWCO, 10,000; Millipore, USA) on Mono-Q 5/50 GL anion exchange columns (Cytiva ...
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bioRxiv - Microbiology 2023Quote: Transepithelial electrical resistance (TEER) of the filter-grown monolayers was measured using a Millicell-ERS device (Merck Millipore) as an index for integrity of the tight junctions (TJ).
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bioRxiv - Bioengineering 2023Quote: ... Unreacted Cy7 NHS Ester was filtered out using a spin filter (MWCO 10 kDa, Millipore Amicon Ultra-0.5). Fluorophore functionalized C7 was mixed with unfunctionalized C7 at a 1:1.5 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasma samples used for EV-RNA isolation with exoRNeasy were pre-filtered (0.2um filter, Millex-GP SLGP033RS, Millipore), following manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >15 mg ml−1 using 30-kDa MWCO centrifugal filter units (Millipore Sigma), aliquoted ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >10 mg mL−1 using a 30 kDa MWCO centrifugal filter (Millipore Sigma), aliquoted ...
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bioRxiv - Bioengineering 2023Quote: ... NPs were purified by centrifugation over a filter (molecular weight cutoff of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The supernatants were passed through a 0.45 μm followed by 0.22 μm PVDF filter (Millipore, Burlington, MA, USA) to remove residual host and bacterial cells ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The stimuli were applied by placing 150 µL of water or odorant on filter paper (Sigma-Aldrich, #WHA10347509) in 35 mm petri dishes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants were filtered through 50 mL syringes fitted with Millex-HV Syringe Filters (Millipore, 0.45 µm, PVDF, SLHVR33RS) and polyethylene glycol solution concentrate was added (5X concentrate ...
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bioRxiv - Plant Biology 2023Quote: ... The eluted protein was concentrated to 1 mg/ml using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma). Size-exclusion chromatography was performed using a Superose 6 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... CopiaGag capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit(EMD Millipore) to a final concentration of 0.8 mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... uptake experiments were performed by using the rapid filtration technique (Tommasini et al., 1996.) with nitrocellulose filters (0,45µm pore size, Millipore) as described by Francisco et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 40,000 g for 1 hour at 4 °C and filtered through 0.45 µm syringe-driven filters (Millipore). The cleared and filtered lysate was loaded onto a 1 ml StrepTrap XT column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: Spontaneous antibody secretion from IgA-switched B cell populations was assessed in MultiScreen 96-well filter plates (Millipore) that were coated with 2 µg/ml goat anti-human Ig (Southern Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... The medium containing the retrovirus was collected and filtered through a 0.45-μm filter unit (Ultrafree-MC; Millipore). The filtered medium was then added to HeLa cells for infection ...
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bioRxiv - Cancer Biology 2023Quote: ... the input media were centrifuged at 3,200 g for 30 min with 3 kDa MWCO filter units (Millipore); after centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: Peak fractions containing GSEC1B were concentrated to 0.04-0.1 mg/ml using a 100 kDa MWCO Amicon Ultra centrifuge filter (Millipore).