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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for Mouse IgG2a Isotype Control Antibody Biotin 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Physiology 2022Quote: ... Primary FSP1 mouse antibody (1:1000 dilution, Cat # 07-2274, 12 kDa) was purchased from EMD Millipore (Burlington, MA). Primary αSMA rabbit polyclonal antibody (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used for immunoblotting: β-Actin (Mouse monoclonal; A5441; AC-15 clone; Lot# 127M4866V; Sigma-Aldrich), Rsad2 (Mouse monoclonal custom made ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatants were analyzed by western blotting with mouse monoclonal antibodies specific for KRAS (1:1000, WH0003845MI; Sigma-Aldrich) and GAPDH (1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatants were analyzed by western blotting using mouse monoclonal antibodies specific to KRAS (1:1000, WH0003845MI; Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 3-5 times with 1x TBST for a total of 20 min and then incubated for an hour with 1:3,000 dilution of secondary antibody (goat anti-mouse alkaline phosphatase conjugate, Sigma). The membrane was washed again with 1x TBST 3-5 times for a total of 20 min and developed using 5-bromo-4-chloro-3-indolylphosphate (BCIP)/ nitro-blue tetrazolium (NBT ...
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bioRxiv - Pathology 2022Quote: ... and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94,Sigma-Aldrich, St. Louis, MO) or 1:1000 anti- CD163 monoclonal antibody (Novus Biologicals ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were washed and then treated with a 1:1000 dilution of mouse anti-FLAG antibody (clone M2, Sigma) for 60 minutes then washed 3x in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Every 10 μg of chromatin was incubated with 2 µg of mouse M2 anti-FLAG monoclonal antibodies (Sigma-Aldrich) overnight at 4° C on a nutator ...
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bioRxiv - Physiology 2023Quote: ... and subsequently used for IP with 48 μg of an anti-NaVPAN mouse monoclonal antibody (mαNaVPAN, Sigma-Aldrich, #S8809), raised against the SP19 epitope (Vassilev et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were incubated with 15 μg of mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich, F1804, RRID: AB_262044) in 1 mL of PBS containing 0.5% BSA and 0.1% Triton X-100 at 4°C for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... the cells were immunostained with a mouse monoclonal antibody against SARS-CoV-1/2 nucleoprotein [clone 1C7C7 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the brain slices were washed 3 times using 1 x PBS in a shaker and incubated in blocking solution (10% normal goat serum and 0.2% Triton X-100 in PBS) for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were rabbit anti-MAP2 (ab281588, abcom) and mouse anti-β-tubulin III (T8578, Sigma-Aldrich). Then ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated in a 1:500 dilution of 4D2 mouse anti-PLSCR1 antibody (Millipore Sigma, cat. #MABS483) in PBS-BG (1x PBS with 0.5% bovine serum albumin and 0.1% glycine ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000, Sigma) and anti-c-Myc rabbit antibody clone 7E18 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... samples stained with primary FBXO11 (mouse) and LONP1 (rabbit) antibodies were incubated with Anti-Rabbit PLUS (Sigma-Aldrich DUO92002) and Anti-Mouse MINUS (Sigma-Aldrich DUO92004 ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody solution at 4 °C overnight (mouse anti- FLAG (1:1000; Sigma-Aldrich; F1804), mouse anti-RpS6 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed 3 times for 5 minutes with TBS-T and incubated in secondary antibody 1:10,000 dilution goat anti-mouse HRP (Sigma).
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bioRxiv - Immunology 2024Quote: ... nonspecific antibody binding was blocked by incubation with an anti-CD16/32 antibody (clone 2.4G2; UCSF Ab production facility) and whole mouse IgG (Sigma) on ice for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was used for a western-blot experiment using a mouse-anti-Puromycin antibody (1:5,000, Millipore MABE343). To detect primary Ab ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore Sigma; MA, USA) or 1:200 goat anti-DCX (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies (insulin rabbit monoclonal, Cell Signaling 3014, 1:3000 and glucagon, mouse monoclonal, Sigma-Aldrich G2654, 1:1000) were applied and incubated for 60 minutes followed by secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... the tissues were incubated with primary mouse anti–α-actinin (sarcomeric) antibody (1:750, Sigma-Aldrich, cat. no. A7811), and vimentin (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated overnight at 4°C in blocking buffer with primary antibody (1:500 mouse anti-Elastin, Millipore Sigma MAB2503), washed ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunostaining for NOTCH3 was performed using mouse monoclonal NOTCH3/N3ECD primary antibody (MABC594, Millipore Sigma, 1:25-1:100) with incubation for 32min following CC1 antigen retrieval for 32min ...
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bioRxiv - Microbiology 2023Quote: The following primary antibodies were used for immunoblotting: mouse monoclonal anti-α- tubulin (#T5168, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-MX1(MxA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Microbiology 2023Quote: ... The secondary horseradish peroxidase (HRP)-conjugated antibodies directed against rabbit or mouse were used at a 1/20,000 dilution (Sigma). Detection of luminescent signal was performed with a Luminata Western HRP substrate kit (Millipore).
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bioRxiv - Microbiology 2023Quote: ... This was followed by probing with secondary HRP conjugated anti-mouse or anti-rabbit antibody (1:2000 dilution, SIGMA). Proteins were visualized using the Clarity Western ECL substrate (Bio-Rad 1705060EM ...
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bioRxiv - Bioengineering 2022Quote: ... and chondroitin sulfate were visualized via staining for anti-collagen type I mouse monoclonal antibodies (Sigma MAB3391, 1:100), anti-collagen II mouse monoclonal antibodies (DSHB II-II6B3 ...
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bioRxiv - Biochemistry 2022Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti rhodopsin clone 4D2 (Sigma-Aldrich: MABN15) and allowed to incubate for 30 minutes then washed with PBS containing 15 mM NaN3 and 2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were then probed overnight at 4°C with the following antibodies diluted in 5% BSA-TBST: Mouse anti-FLAG monoclonal antibody (1:10,000 dilution, Cat #F1804, Clone M2, RRID: AB_262044, Sigma Millipore), Mouse anti-Menin monoclonal antibody (1:2,000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...