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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and commercial bovine serum albumin standards (Thermo Fisher 23208) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and a standard curve using commercial bovine serum albumin standards (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was diluted into 25μL with 5x concentration SYPRO Orange Protein Gel Stain (Sigma Aldrich) in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered protein pellet was further processed for western blot-based analysis and quantitation of TH protein (Millipore, Temecula ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin pre-clearing was done by incubating with Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) for 4 hrs at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Proteins were purified by Nickel-NTA resin and treated with TEV protease (1 mg lab made TEV per 40 mg of protein) and benzonase (100 units/ml, Novagen 70664-3) overnight at 4°C ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Western blot analysis was performed using a primary antibody of rabbit anti-protein A (1:25,000 dilution) (Sigma-Aldrich, St. Louis, MO) and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells used for total protein purification were lysed with 2 ml of a buffer containing 1% Sodium dodecyl sulfate (SDS; Sigma-Aldrich #436143), 50 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... SDS-PAGE and western blot analyses were used to detect proteins and modifications using the following antibodies: anti-phospho SMAD2 (Ser465/467, Sigma, 1:500), Smad2 Polyclonal Antibody (Life Technologies 1:500 ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Biochemistry 2022Quote: ... pH 8.1 (final urea concentration < 2 M) before addition of trypsin (1 µg per 50 µg of protein; Sigma-Aldrich Cat# T6567) for a 3 h incubation ...
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bioRxiv - Neuroscience 2021Quote: ... standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or μ rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01 ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
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bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... protein concentrations in the tissue extracts were measured by using Bicinchorinic Acid Solution / Coppersulfate Solution 50:1 (Sigma-Aldrich, St. Louis, USA). 40 μg of total protein was separated by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500 ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in 5% BSA in TBST and incubated overnight with primary antibodies targeting the following proteins: FLAG (M2; Sigma; 1:1000), phosphorylated tyrosine (P-Y-1000 MultiMab ...
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bioRxiv - Immunology 2022Quote: ... and adhered cells were exposed to exNef or exCont (1 μg/ml protein) for 48 h in the absence or presence of either 20 μM fluvastatin (Sigma, SML 0038) or 10 nM Picropodophyllin (PPP ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were then incubated overnight at 4ºC with primary antibodies for myelin basic protein (1:250 Millipore Cat# MAB384-1ML, RRID:AB_240837), neurofilament 160 kD medium (1:250 ...
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bioRxiv - Biophysics 2024Quote: ... 20 µL volumes of protein were plated on 35-mm dishes precoated with 1% Pluronic F-127 (Sigma Aldrich, St. Louis, Missouri). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were harvested and 0.45 µm filtered before loading on to a 1 ml HiTrap™ Protein G HP column (Cytiva) pre-equilibrated in PBS (79382, Sigma-Aldrich). The column was washed with PBS and bound proteins were eluted using 5 ml of 0.1 M glycine pH 2.5 into 1 ml of 1 M Tris pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were resuspended in cold Ripa buffer supplemented with 1% n-Dodecyl-ß-D-maltoside and complete ethylenediaminetetraacetic acid (EDTA)-free protein inhibitors (Sigma-Aldrich), and incubated at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... 100 ng purified recombinant CTR1-KD or 1 µg MPK4 was mixed with 2 µg Myelin Basic Protein (MBP, a common kinase substrate, Sigma Cat. M1891) and 1 mM ATPγS in kinase reaction buffer (10 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Molecular Biology 2024Quote: ... to alkylation with IAM 200mM and to complete protein digestion with 1 μg of Trypsin (Sigma-Aldrich Inc., St. Louis, MO, USA). The peptide digests were desalted on the Discovery® DSC-18 solid phase extraction (SPE ...
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bioRxiv - Plant Biology 2020Quote: ... antibodies and Protein A agarose beads (Sigma). After elution of immune complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were blotted onto Immobilon-P (Millipore) with a semi-dry blotting apparatus (TRANS-BLOT SD ...
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bioRxiv - Cancer Biology 2021Quote: ... protein G beads (EMD Millipore, #16-266), biotin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to PVDF membrane (Millipore). The membrane was cut into strips corresponding to the molecular weight of proteins of interest ...
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bioRxiv - Cell Biology 2020Quote: ... Polyclonal rabbit-anti-Protein A antibody (Sigma) and monoclonal mouse-anti-TAC102 antibody were used as described above ...