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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Molecular Biology 2019Quote: Purified proteins were concentrated to the indicated protein concentration described in the text using Amicon Ultra centrifugal filters (10K MWCO, Millipore) and added to solutions at varying concentrations with the indic ated final salt and molecular crowder concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Cell Biology 2020Quote: ... The target protein was detected by specific primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated antibodies (for less abundant proteins) (Sigma) or fluorescence-labeled secondary antibodies (for abundant antigens ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for target proteins with specific antibodies against phosphorylated or total proteins and secondary antibodies before being developed using Immobilon chemiluminescent substrate (Millipore) and visualized using the Odyssey Infrared Imaging System (LI-COR).
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Physiology 2023Quote: ... All images were normalized total protein from the same membrane stained using Amido Black total protein-stained membrane (A8181, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... and 50 µL was taken as protein input while the remaining lysate was pre-cleared using 100 µL or Protein G Sepharose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Instrument performance was monitored daily using a system suitability test data consisting of a 30-minute gradient injection of 30fm BSA and 60fm of casein protein digest (both standards were trypsin digested in-house from commercial protein stocks (Sigma)) that was run before each sample ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Physiology 2023Quote: ... Supernatants containing equal amounts of soluble proteins were mixed with 2x SDS protein sample buffer containing 100mM DTT (Sigma-Aldrich), denatured by incubating on a heat block at 98 C for 5 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Protein STOP1 was selectively enriched from the total protein extract using Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823). Subsequently ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were concentrated with Centricon filters (Merck-Millipore) when needed and/or diluted to a final concentration of 1 mg/mL in PBS.
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bioRxiv - Cancer Biology 2021Quote: ... and proteins were transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk or 5% BSA in TBST (Tris-buffered saline containing Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Sigma) and incubated in 5% milk powder in PBS 0.1% Tween-20 (0.1% PBST ...
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bioRxiv - Immunology 2021Quote: ... affinity purified by protein A (HiTrap, Sigma-Aldrich), and tested for endotoxin levels (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-Protein A (Sigma-Aldrich, cat. # P2921) were used ...
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bioRxiv - Immunology 2021Quote: ... and proteins were concentrated using 10kDa concentrators (Millipore).
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bioRxiv - Genetics 2021Quote: ... proteins were transferred to PVDF membranes (Immobilon, Millipore) and blocked in TBS containing 5% skim milk and 0.1% Tween-20 ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were transferred to PVDF membrane (Millipore) and incubated with blocking buffer (4 % skim milk in 1 × PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were concentrated using Vivaspin columns (SIGMA) and quantified using the Bradford assay (SIGMA) ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transfered to a PVDF membrane (Millipore) at 23 volts for 0.5 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was measured with BCA (Sigma-Aldrich), according to manufacture instructions ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed using protein lysis buffer (Sigma) with protease and phosphatase inhibitor cocktails (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Monoclonal IgG directed against UBE3A protein (E8655, Sigma) and anti-β-ACTIN monoclonal antibody (A5316 ...
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bioRxiv - Molecular Biology 2021Quote: ... then pre-cleared with protein G beads (Sigma). Protein lysates were incubated with either anti c-MYC agarose beads (Sigma A7470) ...
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bioRxiv - Molecular Biology 2020Quote: ... protein extraction was performed using RIPA buffer (Sigma) supplemented with protease/phosphatase inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Molecular Biology 2020Quote: Protein extraction using trichloroacetic acid (TCA, Sigma T0699) and western blotting methods are similar to those described in (12) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a PVDF membrane (Millipore), which was then blocked in 5% milk in PBS + 0.2% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Protein was transferred onto activated PVDF membrane (Millipore) using trans-blot Turbo system (Biorad) ...
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bioRxiv - Genomics 2019Quote: ... Protein G agarose beads (Millipore Sigma, #16-266) were washed twice with PBS and 100 μg of anti-biotin antibody (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were concentrated by ultrafiltration (Amicon 30K, Millipore). The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... 500 U/mL LIF Protein (Millipore Sigma ESG1106), 3 µM CHIR99021/GSK-3 Inhibitor (Millipore Sigma 361571) ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was transferred to PVDF membrane (Millipore) and processed for blotting ...