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Citations for Millipore Sigma :
3551 - 3600 of 4323 citations for Recombinant Human IL12B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Frozen-thawed human blastocysts (5-6 days post-fertilization) were gently treated by acidic Tyrode’s solution (Sigma, T1788) to remove the zona pellucida 4 ...
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bioRxiv - Microbiology 2023Quote: ... and human embryonic kidney (HEK) 293T or 293 cells were cultured in Dulbecco’s modified essential medium (DMEM) (Sigma) with 10% foetal calf serum (FCS ...
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bioRxiv - Pathology 2023Quote: Sub confluent human RPE cells were treated with 500 μM H2O2 (Sigma-Aldrich #H1009, Saint Louis, MO, USA) after sci control or siRNA transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were cultured in ImmunoCult-XV T cell expansion medium supplemented with human serum (10%; Sigma, USA), Penicillin-Streptomycin and IL-2 (100 IU/ml ...
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bioRxiv - Microbiology 2023Quote: ... Carbohydrate sources for the human fecal matter inoculated cultures were apple pectin (2.5 g/L, Sigma-Aldrich, USA) and porcine mucin (2.5 g/L ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL AF647 anti-human VEGF antibody (Sigma) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Cell Biology 2022Quote: esiRNAs specific to human caveolin-1 or ARHGAP29 and a non-targeting esiRNAs were purchased from Sigma-Aldrich and transfected at a final concentration of 50 nM using Dharmafect (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Petrisoft™ easy coat hydrogel 0.2 kPa or 50 kPa 6-well plates were coated with 5μg/ml human fibronectin (Sigma) and RNA lysates harvested as described.
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... spermatozoa from JF1/MsJ males were capacitated in Human Tubal Fluid medium (Merck Millipore, Cat#MR-070-D) supplemented with 10 mg ml−1 Albumin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The cleavage fragments were analyzed by western blot with either mouse anti-human fibrinogen polyclonal antibody (Sigma-Aldrich) at a 1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated anti-mouse IgG Fab (Cat# A9917) and anti-human IgG Fab (Cat# SAB4200791) were from Sigma. HRP-conjugated anti-mouse IgG (Cat# PA1-28568 ...
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bioRxiv - Neuroscience 2023Quote: ... 50mg fresh frozen postmortem human brain tissue was homogenized in Nuclei EZ Lysis buffer (NUC101-1KT, Sigma-Aldrich) and incubated for 5 min before being passed through a 70µm filter ...
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bioRxiv - Bioengineering 2023Quote: ... we pre-treated 48-well plastic plates with 200 μL of 20 μg/mL human fibronectin (Milipore Sigma) for 10 minutes at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: E0.5 embryos were cultured in embryo culture medium (EmbryoMax® Human Tubal Fluid, Sigma-Aldrich, # MR-070-D) under mineral oil for embryo culture (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... HUDEP-2 were maintained in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 330 μg/mL human holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at 8-8.5×106 cells in 75 cm² or 16.5×106 cells in 150 cm² culture flasks (TPP) coated with 0.25% fibronectin from human plasma (Sigma) and 1% extracellular matrix from Engelbreth-Holm-Swarm murine sarcoma (Sigma ...
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured using Fibronectin/Collagen/BSA ECM mixture-coated petri dishes (10µg/mL Human Fibronectin, Sigma Cat. No. F2006; 100µg/mL BSA, Sigma Cat.No.126575 ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from ATCC (TIB-202) and LX-2 human stellate cells were purchased from Sigma Aldrich (SCC064). LSECs were cultured in endothelial cell basal medium-2 (EBM-2 ...
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bioRxiv - Biophysics 2024Quote: ... For microfluidic surface endothelialization the channels were incubated overnight at 4°C with 10% human fibronectin (Sigma-Aldrich). HUVECs (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... Baby Hamster Kidney (BHK) and Human Embryonic Kidney (HEK) 293 were grown in Dulbecco’s modified eagle’s medium (DMEM; Sigma) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated in 48-well plates pre-treated with 50 mg/mL human fibronectin (FC010-10MG, Millipore) and incubated for 24 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... To this purpose culture dishes/glass slides were first coated with fibronectin (Sigma-Aldrich, Darmstadt, Germany, human plasma) at 5 µg/cm2 concentration diluted in sterile PBS ...
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bioRxiv - Immunology 2024Quote: ... purified human Tsen were pre-treated with or without the proteasomal inhibitor MG132 (10 µM; M7449; Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin supplemented with 10 ng/ml EGF (Epidermal growth factor human, Sigma-Aldrich, Cat#E9644) and FGF (Fibroblast growth factor basic human ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... Micropatterned coverslips were functionalized with a solution of 10 µg mL-1 FN from human plasma (Sigma Aldrich #F1056) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... TIFs (Telomerase immortalised fibroblasts29) and human embryonic kidney (HEK)293Ts cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (D5796, Sigma). All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... 1×105 ESCs were plated on a 3.8 cm2 plate coated with human plasma fibronectin (Millipore, cat. no. FC010) and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... and 5 μm sections were prepared and incubated with a mouse anti-human IgG4 mAb (clone MRQ-44; Sigma) or isotype control ...
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bioRxiv - Synthetic Biology 2019Quote: ... we grew the cells in plastic 48-well plates that were pretreated with 50 μg/mL human fibronectin (Millipore) for at least 10 min at 37 °C before cell plating (to improve cell adherence) ...
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bioRxiv - Cancer Biology 2019Quote: The AsPC-1 and Capan-2 human PC cell lines were obtained from Sigma-Aldrich (St. Louis, MO, USA) and Thermo Fisher (Waltham ...
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bioRxiv - Neuroscience 2019Quote: ... the eye cup was treated for ∼ 15 minutes with human plasmin (∼ 50 µg mL−1, Sigma or Haematologic Technologies) to aid vitreous removal.
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bioRxiv - Biochemistry 2020Quote: ... Other antibodies used in this study include those specific to human CD3 (UCHT1 clone, Cat. number 217570, Merk-Millipore), Vav1 (Cat ...
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bioRxiv - Microbiology 2019Quote: ... a human telomerase immortalised retinal pigment epithelial line was passaged in Dulbecco’s modified minimal essential medium (DMEM)-F12 (Sigma) supplemented with 200 mM glutamine ...
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bioRxiv - Immunology 2019Quote: ... monocytes were cultured at 1 × 106 cells/ml in RPMI-1640 medium supplemented with 1% human AB serum (Sigma), 20 ng/ml M-CSF (Peprotech) ...
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bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... an additional blocking step was performed by incubating cells with 2 mg/ml γ-globulins from human blood (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were washed with 60x slurry volume in lysis buffer and eluted through overnight incubation at 4°C with 160μl of Human Rhinovirus (HRV)-3C protease (Millipore) in a final volume of 2-3ml ...
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bioRxiv - Bioengineering 2020Quote: ... Three sections at varying depths within the tumor are immunostained with mouse anti-human nuclei (clone 235-1, Millipore) followed by secondary Dylight 488 horse anti-mouse (Vector) ...
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bioRxiv - Synthetic Biology 2021Quote: ... IPSCs were plated at 25,000 cells/cm2 in a 24-well plate coated with 15µg/ml human laminin (Sigma, USA). The following day ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded onto glass coverslips coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation) at a density of 80,000 cells and 40,000 cells for the PDGFRα homodimer cell line and PDGFRβ homodimer cell line ...
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bioRxiv - Cancer Biology 2020Quote: Human AGCT-derived KGN cells (Riken, Tsukuba, Japan) and JGCT-derived COV434 cells (Sigma-Aldrich, St. Louis, MO, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-Ham’s F12 (Caisson ...