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Citations for Millipore Sigma :
3551 - 3600 of 10000+ citations for Catenin alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... A luciferase solution containing recombinant luciferase from Photinus pyralis (Sigma-Aldrich, 20 μg/mL), ATP disodium trihydrate (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Iba-1 (FUJIFILM Wako, dilution 1:500) and mouse monoclonal antibody directed against GAD67 (Millipore Sigma, dilution 1:500). For the secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-c-Jun (Thr91, cell signal, 2303, 1:1000), rabbit anti-c-Jun (cell signal, 9165, 1:1000) and mouse anti-GAPDH (Sigma, Cat: G8795, 1:1000). Immunoreactivity was visualized using appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with a mixture of rabbit anti-P2X7R (1:500) and goat anti-IBA-I (1:1000)/mouse anti-GFAP (1:2000, #G6171, Sigma-Aldrich, St. Louis, MO)/rabbit anti-NeuN (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... IRDye 800nm CW Goat anti-Mouse (Li-Cor) at a 1:10,000 dilution for Muc5b or rabbit antilectin UEA-1 (Sigma, U4754) at a 1:1,000 dilution in blocking buffer for Muc5ac was added to the mucin blot and incubated at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: Immunocytochemistry was carried out on fixed unpermeabilized cells with polyclonal antibodies anti-Xlenpp4 used at 1/200 or anti-mEnpp4 (CR65; see31) used at 1/400 and anti-rabbit IgG FITC (Sigma) at 1/80 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then fixed and rat anti-MBP (1/5) [11] and rabbit anti-NF-H (1/1000, EMD Millipore) primary antibodies were added o/n at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody against vinculin was purchased from HRP-conjugated antibodies against mouse IgG (NA931V; 1:10 000) and rabbit IgG (NA934V; 1:10 000) were purchased from Sigma. Immunohistochemistry was performed by Molecular Pathology (Robarts Research Institute ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Immunology 2019Quote: ... Secondary HRP-conjugated antibodies (polyclonal goat anti-mouse IgG at 1/1000 or anti-rabbit IgG at 1/2500 from Millipore) were used to detect antibody binding ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with primary antibodies (rabbit anti-miniSOG 1:500 [Tsien Lab]; guinea pig anti-VGLUT2 1:2000 [Millipore #AB2251] ...
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bioRxiv - Cell Biology 2021Quote: ... followed by horseradish peroxidase (HRP)- conjugated swine anti-rabbit (1:5000) while Mrc1-FLAG in input samples was detected by HRP-conjugated rabbit anti-FLAG (1:3000, A8592, Sigma). Rabbit anti-Ctf4 (1:2000 ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901]; mouse anti-Pax6, 1:200 [Millipore #MAB5552] ...
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bioRxiv - Biochemistry 2020Quote: ... membranes were incubated for 1 hour at room temperature with 1:10,000 diluted peroxidase-conjugated goat anti-rabbit IgG (Sigma, A6154). After 4 washes in PBS with 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated with secondary antibodies (mouse, 1:10000 dilution, A9044, Sigma; rabbit, 1:10000 dilution, A9169, Sigma). Immunoreactive bands were visualized by ChemiDoc XRS+ imaging system ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal, 1:1000 dilution, Sigma), α-tubulin (mouse monoclonalDM1A ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in 3% normal donkey serum/0.25% Triton X-100 in PBS for 1 h at room temperature and then incubated overnight at room temperature in blocking solution containing primary antiserum (rat anti-mCherry, Invitrogen M11217, 1:1,000; chicken anti-GFP, Life Technologies A10262, 1:1,000; rabbit anti-vasopressin, Sigma-Aldrich AB1565 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.3% Triton X-100 in PBS for 1 hour and then incubated with rabbit anti-TH (1:1000; Millipore: AB1542) and sheep anti-fibrinogen (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were stained with rabbit anti-Actin or rabbit anti-TnI (1:1000; a gift from A. Ferrus) or mouse anti-Tubulin (1:1000, Sigma) and washed with TBS-Triton X (0.1%) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in 3% normal goat serum in PBS for 1h and incubated overnight at 4°C with the following primary antibodies: (1) rabbit monoclonal antibody against NeuN (1:1000, MAB377, Millipore), (2 ...
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bioRxiv - Neuroscience 2019Quote: ... We then removed the supernatant and incubated the lysates on ice for 1 hour with rabbit antibody to D2 receptors (1 μg per 100 μL, Millipore). Anti-rabbit Ig IP beads were added and samples were incubated overnight at 4°C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-synaptophysin (1:500; SMC-178D, StressMarq Bio.) and rabbit monoclonal anti-GluR1 (1:500; 04-855, Millipore). Secondary antibodies were Alexa Fluor 488-donkey anti-mouse IgG (1:300 ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The grids were washed once with 1% BSA in DPBS and incubated with a 1:10 dilution of rabbit anti-c-Myc antibody (Sigma) for 1 h ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... Tubulin as loading control was detected with anti-tubulin (1:10000, MBL) and HRP-conjugated anti-rabbit IgG as the secondary antibody (1:10000, Sigma). For detecting the heperphosphorylation of Crt1-13MYC ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked (for 1 hr) and incubated for 1 hr with primary rabbit anti-FLAG antibody (Sigma-Aldrich). Following buffer wash the membrane was incubated for 1 hr with HRP-conjugated IgG mouse anti-rabbit antibodies (ThermoFisher Sci.) ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Genetics 2020Quote: ... 4 RIMBP1 WT and 4 RIMBP1 KO cerebelli were cut parasagittal (30 um) and stained with antibodies anti-GAD65 (1:1000, mouse, DSHB) and anti-calbindin (1:1000, rabbit, Millipore). Synaptic puncta were imaged using a Nikon confocal microscope (A1RSi+ ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated with PBS + 1% milk solution containing a 1:10.000 anti-rabbit horseradish peroxidase conjugated secondary antibody (A0545, Sigma-Aldrich). The membrane was washed three times with PBS + 0.05% Tween-20 and then incubated for 5 min with Pierce ECL Plus substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: (1) rabbit anti-gamma amino butyric acid (GABA; Sigma-Aldrich #A2052, 1:1000 dilution) (2 ...
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Cancer Biology 2022Quote: ... rabbit anti-human βIII-tubulin (1:500; Covance, cat. MRB-435P) and α-tubulin (1:500; Sigma-Aldrich, cat. T9026). Primary antibodies were tagged using either goat anti-mouse AF647 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used: Rabbit anti-Orai1 (1:50, from Dr. Stefan Feske) and mouse anti-ORAI1 (1:50, SAB3500126, SIGMA), rabbit anti-STIM1 (1:200 ...