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Citations for Millipore Sigma :
3501 - 3550 of 4914 citations for Recombinant Human CEACAM1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.15×106 of murine stromal cells expressing human delta like ligand 1 (Sigma) were combined with 7.5×103 of sorted and enriched KO-/- ...
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bioRxiv - Biochemistry 2023Quote: ... Zymosan A was opsonized by incubation with human serum (Sigma-Aldrich, no. H4522) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... human iPSCs were cultured on gelatin-coated dishes in differentiation medium (GMEM (Sigma) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... MDM were cultured in RPMI 1640 medium supplemented with 10% human serum (Sigma). VSVDG pseudotyped HIVD1 GFP virus was produced by transfecting of 293T with GFPDencoding genome CSGW ...
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bioRxiv - Pathology 2023Quote: Human hepatoma cell lines were grown in Minimum Essential Medium (MEM, Sigma Aldrich) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... The shRNAs targeting human MARCHF8 were ordered from Sigma-Aldrich (St. Louis, MO). The sgRNAs targeting Marchf8 were designed using the web-based software ChopChop (chopchop.cbu.uib.no ...
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bioRxiv - Cell Biology 2023Quote: ... glass coverslips were coated with 10 µg/mL human plasma fibronectin (FC010, Millipore) overnight at 37◦C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected with 270ng of esiRNA Human KCNJ2 (Cat. EHU019661, Millipore Sigma) or non-targeting control (NTC ...
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bioRxiv - Immunology 2024Quote: ... MILLIPLEX Map Human cytokine/chemokine Magnetic Bead Panel – Premixed 41 Plex (Millipore Sigma), run on a MAGPIX® xMAP instrument according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human spinal cord samples were homogenized with a dounce homogenizer (Sigma Aldrich, D8938) in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich ...
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bioRxiv - Cell Biology 2024Quote: ... primary human skeletal muscle cells were seeded and differentiated on laminin (Sigma Aldrich) coated 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... We obtained the antibodies through commercial suppliers: IgA from human serum (Sigma-Aldrich), IgM from human serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: Fresh or cryopreserved primary adult human hepatocytes (BeCytes Biotechnologies, BiolVT or Sigma-Aldrich) were resuspended in hepatocyte spheroid medium (HSM) ...
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips with C3bi-coated beads were incubated with rabbit anti-human IgM (Sigma) at 1:250 ...
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bioRxiv - Microbiology 2024Quote: Purified human PMNs were incubated with phorbol 12-myristate 13-acetate (PMA) (Sigma) 100nM for 4h ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Neuroscience 2024Quote: ... we used our LPS-sensitized model of HI described above and delivered IP 1.5 IU/g of hCG diluted in normal saline (Sigma-Aldrich, St. Louis, MO, Catalog no. CG-10) or normal saline alone as vehicle control two hours prior to LPS administration (Figure 2A) ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham, Dorset, UK; 2% BSA, Sigma-Aldrich, Gillingham, Dorset, UK in PBS). Anti-filaggrin primary antibody staining (mouse monoclonal 15C10 from Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Fixed brain samples were placed securely one a time in an MR-compatible holder immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, St-Louis, MO, USA). In order to assess volume differences throughout the mouse brain ...
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bioRxiv - Biochemistry 2021Quote: J774A.1 murine macrophage-like cells were grown in tissue culture flasks with hydrophobic surface for suspension cells in DMEM/FCS (DMEM high glucose medium (Sigma-Aldrich, St. Louis, United States) supplemented with 2 mM glutamine and 10% heat-deactivated FCS) ...
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bioRxiv - Microbiology 2021Quote: ... the MDCK monolayers were washed twice to remove FCS using PBS containing 1 μg/mL of trypsin treated with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) (Sigma-Aldrich, St. Louis, Mo, USA), inoculated with each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Genetics 2021Quote: ... the brains were removed from the fixative and placed into plastic tubes (internal diameter 1 cm, volume 13 mL) filled with a proton-free susceptibility-matching fluid (Fluorinert® FC-770, Sigma-Aldrich, St. Louis, MO). Images of excised brains were acquired on a 7T BioSpec animal MRI system (Bruker Biospin MRI GmbH ...
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bioRxiv - Genetics 2022Quote: ... the brains were removed from the fixative and placed in plastic tubes (internal diameter 1 cm, volume 13 mL) filled with a proton-free susceptibility-matching fluid (Fluorinert® FC-770, Sigma-Aldrich, St. Louis, MO). Images of excised brains were acquired on a 7T BioSpec animal MRI system (Bruker Biospin MRI GmbH ...
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bioRxiv - Microbiology 2023Quote: ... used in this study were previously cultivated in a rabbit kidney cell lineage (RK-13, ATCC CCL-37) in RPMI medium supplemented with 10% fetal calf serum (FCS-Sigma-Aldrich, St. Louis, MO, USA) and gentamicin at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Genomics 2024Quote: ... The reaction products were analyzed on 8% PAGE-SDS gels with silver staining or Western blotting for PAR using a recombinant anti-poly-ADP-ribose binding reagent (Millipore, Cat# MABE1031; RRID: AB_2665467).
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM recombinant calmodulin (Enzo, cat. BML-SE325-0001) and 2 mM Adenosine 5’-triphosphate (ATP) disodium salt hydrate (Sigma-Aldrich, cat. A1852-1VL) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...