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Citations for Millipore Sigma :
3501 - 3550 of 4328 citations for Recombinant Human CD2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... suspended in a solution prepared in the following order: 30 μL of human male AB serum (Sigma-Aldrich), 60 μL human plasma fibronectin (EMD Millipore) ...
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bioRxiv - Bioengineering 2022Quote: ... KCs were cultured and serially passaged into 7 μg/cm2 human collagen IV-coated plates (#C7521; Sigma-Aldrich). All cell sources were obtained at Yale-New Haven Hospital under protocols approved by the Yale University Institutional Review Board including ...
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bioRxiv - Biophysics 2022Quote: ... for mouse ILC2s and human peripheral ILC2s was supplemented with MEM nonessential amino acid solution (M7145, Sigma-Aldrich), 10 mM HEPES buffer (H3537 ...
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bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were transduced in mTESR in the absence of antibiotics and with 6 μg/mL polybrene (Millipore). The lentiviruses containing the GOIs were transduced using 10 MOI in the presence of the reverse tetracycline transactivator M2rtTA (MOI 10) ...
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bioRxiv - Genomics 2019Quote: ... was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% AB human serum (Sigma Aldrich), 100 U/mL recombinant human IL-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Cell lines and isolated primary human immune cells were treated with etoposide (ETP, Sigma-Aldrich, cat. number E1383) at the indicated concentrations from a 50 mM stock that was prepared from a lyophilized powder reconstituted in DMSO (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Cell Biology 2020Quote: Human monocyte-derived macrophages were separated from healthy donor buffy coats by centrifugation through a Histopaque 1077 (Sigma) gradient and adhesion purification ...
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bioRxiv - Immunology 2020Quote: ... Cell enrichment buffer consisted of 0.22 μm filtered PBS supplemented with 0.5% heat inactivated human AB serum (Sigma).
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bioRxiv - Biophysics 2021Quote: ... Samples were stained using a 1:100 dilution of the primary antibodies (rabbit polyclonal anti-human IRSp53, Sigma and rabbit polyclonal anti-human IRTKS antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in Human Plasma-like Media (HPLM) (8) + 1% pen/strep + 5% dialyzed serum (Sigma Aldrich). For SARS-CoV-2 peptide stimulation ...
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bioRxiv - Microbiology 2022Quote: ... The Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Immunology 2019Quote: ... Total antibody concentration between determinants was controlled by addition of human IgG2/kappa where needed (Sigma Aldrich; AG504). Cells were washed with ice-cold PBS and added to dengue immune complexes (MOI 5) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza ...
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bioRxiv - Genomics 2020Quote: Human induced pluripotent stem cells (hiPSCs) were thawed with mTeSR1 (STEMCELL, #85850) and 1 μM H1152 (Millipore, #555550), seeded on Matrigel (Corning ...
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bioRxiv - Physiology 2021Quote: ... C2C12 myotubes were cultured in the differentiation medium containing 2 or 200 nM human insulin (Cat.# I9278, Sigma) for 16 hours prior to reaching day 10 (Fig.1A) ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: Overnight cultures were standardised to an OD600nm of 0.1 and incubated in 10% pooled human serum (Sigma Aldrich) diluted in PBS for 90 min at 37°C with shaking ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2021Quote: Human muscles were dissociated and digested with pronase enzyme (1.5mg/mL; protease from streptomyces griseus P5147-5G Sigma) in DMEM at 37°C for 20 min and passed through a 100 mm cell strainer (repeated 4 times) ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Human osteosarcoma U2OS cells (ATCC® HTB-96™) were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Developmental Biology 2021Quote: ... Human NTOs were permeabilized and blocked using .3% Triton X (PanREAC AppliChem) and 0.5% BSA solution (Sigma-Aldrich) for 30 m ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 -Clonetics ...
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bioRxiv - Microbiology 2020Quote: ... Serum susceptibility was determined by incubation of late log-phase cells with 66 % normal human serum (Sigma-Aldrich) as described (Short et al ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-human RIPK3 (clone 1H2; produced in-house5 and available as Millipore MABC1640; 1:2000/1:400), rabbit anti-phospho-S227 human RIPK3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were cultured in DMEM (high glucose, Sigma-Aldrich, SH30022.01) with 10% FCS (Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... and human eEF1A1 (Horizon Discovery) was cloned into the NdeI and XhoI restriction sites of pET30a (Millipore Sigma). Quikchange mutagenesis (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... The stability of the purified radioimmunoconjugate [225Ac]DOTA-YS5 (0.05 mL, 7.43 µCi) was verified by incubating with human serum (0.45 mL; Sigma Aldrich) and 0.9% Saline (0.45 mL ...
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bioRxiv - Microbiology 2022Quote: ... the apical channel was incubated with collagen IV (30 μg/mL) from human placenta (Sigma, cat. no. C7521) and collagen I (200 μg/mL ...
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bioRxiv - Microbiology 2022Quote: Pulmonary A549 adenocarcinoma human alveolar basal epithelial cell lines [13] were obtained from ECACC (#86012804, Sigma-Aldrich, Germany) and cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Microbiology 2022Quote: ... 72) and diluted to OD600nm 0.1 in defined medium supplemented with 5% v/v human serum (Sigma-Aldrich). OD600nm was monitored at 3 ...
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bioRxiv - Microbiology 2022Quote: ... ubiquitin carboxyl-terminal esterase L1 (UCHL1) (29) using rabbit anti-human UCHL1 (Sigma, HPA005993 at 1:1,000 dilution). The secondary antibody was Alexa Fluor 594-conjugated goat anti-rabbit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney cells (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Catalog no. D1152; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... 1 g/kg body weight) or human regular insulin solution (1 units/kg, catalog no. 19278; Sigma-Aldrich) was administrated intraperitoneally and blood glucose was measured from the tail vein at 0 ...
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bioRxiv - Molecular Biology 2022Quote: HeLa and human embryonic kidney (HEK) 293 cells were cultured in Dulbecco’s Modified Eagle’s medium (high glucose, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: Control siRNA (siRNA scr) (siRNA Universal Negative Control #1 SIC001) and human GPC3 siRNA (NM_004484; SASI_Hs01_00205845) were purchased from Sigma-Aldrich and used at a concentration of 50 nM ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Cell Biology 2024Quote: ... the media was removed and monolayers fixed with methanol prior to antibody addition targeting human collagen I (Sigma) followed by AlexaFluor 488 secondary antibody (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse embryos and human embryos were cultured in droplets of pre-conditioned KSOM (Sigma-Aldrich, MR-121-D) and GTL (Vitrolife ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured using Fibronectin/Collagen/BSA ECM mixture-coated petri dishes (10µg/mL Human Fibronectin, Sigma Cat. No. F2006; 100µg/mL BSA, Sigma Cat.No.126575 ...
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bioRxiv - Immunology 2023Quote: ... AP-labeled goat anti-human IgG (γ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) and AP-labeled goat anti-human IgA (α-chain specific ...
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...