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Citations for Millipore Sigma :
3501 - 3550 of 10000+ citations for Natural Cytotoxicity Triggering Receptor 1 NCR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were diluted in 0.1 % Triton X-100 in PBS (β - tubulin III: ab78078, abcam, 1:500; MAP2: M4403, Sigma, 1:500; PAX6: 60094, StemCell, 1:500) and incubated on the CO sections in a humidified chamber at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was blocked with 5% milk powder in TBST for 1 hour and probed with primary antibodies α-GFP (1:5000, g1544, Sigma Aldrich), α-myc (1:5000 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... Organoids were incubated for 2 days with primary antibodies (Anti-GFP at 1:100, A11120, Thermo Fisher Scientific; Anti-Otx2 at 1:100, HPA000633, Sigma-Aldrich) diluted in 1% NDS in PBS-T 0.2% ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381; Mouse anti-β-Tubulin, 1:2000, Sigma T5168) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies were rabbit anti-phosphorylated ERK (1/500; Tasaki et al., 2011a) or mouse anti-α-tubulin monoclonal antibody DM 1A (1/5,000; Sigma-Aldrich, T9026). An appropriate antibody conjugated with horseradish peroxidase (1/5,000 ...
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bioRxiv - Neuroscience 2022Quote: ... whereas transfected Flag-Glt-1 cells were incubated overnight with a mouse anti-Flag® primary antibody (1:200-Sigma Aldrich; #F1804) that recognized the DKK sequence ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and their concentrations were as follows: 1:1000 mouse-anti-α-SMA (Millipore Sigma; Burlington, MA; clone ASM-1/1A4), 1:300 rabbit-anti-Daam1 (Proteintech ...
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bioRxiv - Molecular Biology 2019Quote: ... Following fixation with cold methanol and blocking with 1% BSA, presence of RAGE in the nucleus was confirmed using immunofluorescence (primary antibody, 1:200 goat anti-RAGE (Millipore, CA, USA), 1:5000 secondary antibody donkey anti-goat 488 (LICOR ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was blocked with 3% BSA/Tris-buffered saline that contained 0.1% Triton X-100 (TBST) and was incubated with a 1:500 dilution of anti-PUM1 rabbit polyclonal antibody (Sigma-Aldrich, #HPA027424), 1:250 dilution of anti-PUM2 rabbit monoclonal antibody (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:400 mouse anti-light chain clathrin antibody (Sigma Aldrich, C1985) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...
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bioRxiv - Biophysics 2019Quote: ... Then they were incubated again for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and NURF-1.F isoform with FLAG tag was detected using 1:1000 Millipore ANTI-FLAG antibody (Millipore Sigma, Catalog number: F3165).
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bioRxiv - Neuroscience 2019Quote: ... retinas were incubated in primary antibodies (1:1000 rabbit anti-GFP, Invitrogen, Grand Island, NY; 1:500 goat anti-ChAT, Millipore, Billerica, MA) for 1-3 days ...
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bioRxiv - Bioengineering 2022Quote: Specimens were permeabilized with 4% paraformaldehyde and incubated for 1 hour at room temperature in primary antibody anti-vinculin (1:500, Sigma Aldrich, V4505), secondary antibody Alexa Fluor 488 (1:600 ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (1:500, anti-GLT (gift from Dr. N.Danbolt) and anti-Nischarin (1:100, Sigma, Cat. No: HPA023189). For control PLA ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with primary antibodies, GFP (Novus NB1001770, 1:100) and NeuN (EMD Millipore MAB377, 1:150) in 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... All four antibodies were used together (1:200) for staining protocols in sequential stainings with Chicken anti-neurofilament 200 (NF200, Millipore, 1:1000), Rabbit anti-S100 (Dako ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was subsequently blocked using 1% casein in 1x PBS and incubated with primary antibody solutions with following dilutions: mouse anti-HA (Sigma, 1:500), rabbit anti-HSP101 (r950 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibodies in blocking buffer in a humidified chamber for 1 h: anti-FLAG M2 (1:2000 dilution, Sigma Aldrich, F3165) and an endogenous compartment marker antibody from rabbit (Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Neuroscience 2021Quote: ... Then the membrane was stripped and probed again with primary antibodies (p44/42 MAPK, Cell Signaling 9102, 1:4000 dilution, β-actin 1:10,000, A5316, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with PBS and incubated with secondary antibody and primary conjugated α-smooth muscle actin antibody (1/1000 dilution, 1 hour, room temperature, A-11034; Molecular Probes, Eugene, USA. C6198; Sigma) before counterstaining with DAPI (5 min ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated in rabbit anti-Iba1 (1:1000, Wako, Cat.#019-19741) and chicken anti-GFAP antibody (1:2000, Sigma, Cat#AB5541) dissolved in PBST at 4 °C for 24 hours ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... and 0.4% Triton X-100 in Tris-bufered saline (TBS) at room temperature (RT) for 1 h and then incubated with primary antibodies against Sftpc (AB3786, Millipore; 1:500), Pdpn (8.1.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... of fixed slice cultures were prepared and stained with antibodies against Iba1 (rabbit, 1:1000; Fujifilm Wako #019-19741) and GFAP (mouse, 1:1000; Sigma-Aldrich #G3893). Slices were incubated in appropriate Alexa Fluor® dye-conjugated secondary antibodies (donkey ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were used at 1:1000 and included: mouse monoclonal anti-β1/β2-adaptins (clone 100/1; Sigma, St. Louis, MO); mouse monoclonal anti-clathrin heavy chain (clone 23 ...
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bioRxiv - Developmental Biology 2020Quote: ... washed with TBS-T and incubated overnight with primary antibodies (anti-Actin 1:10000 Santa Cruz Biotechnology #sc1616; anti-Dab1 1:2000 Sigma-Aldrich #HPA052033 ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Plant Biology 2021Quote: ... The glutathionylated VirE2 was detected by the anti-glutathione monoclonal primary antibody (My biosource, 1:1000) and anti-mouse (Sigma, 1:1000) secondary antibody (Klaus et al ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibody was diluted 1:100 in blocking buffer (rabbit-α-LAMP1: 1:100; rabbit-α-mTOR (HPA071227, Sigma-Aldrich Co. LLC): 1:12.5 ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Neuroscience 2020Quote: Immunostaining for ChAT or TH was performed using the following antibodies: rabbit anti-TH 1:200 (Pel-Freez cat # P4010-0) or goat anti-ChAT 1:25 (Millipore cat # AB144P). Sections were fixed in an acetone/methanol mixture (1:1 ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight followed by goat anti-rabbit secondary antibody conjugated with Cy3 for 1 hr at room temperature (1:500, Millipore, Cat. #AP132C). Since the purpose of this staining was to evaluate the presence of extracellular HMGB1 in the tendon ...
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bioRxiv - Cell Biology 2021Quote: ... then washed twice in PBS and incubated 1 hour with the tertiary antibody goat anti-mouse whole molecule conjugated to FITC (Sigma, 1:400). The intrabody anti-GFP nanobody 3 x HA-tagged (INbGFP::3HA ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were rinsed three times with PBS and incubated 1 hour at RT with secondary antibodies and DAPI (1/10000, Sigma-Aldrich D9564) diluted in the same solution and rinsed 3 times with PBS before mounting on slides with VectaShield® Vibrance mounting medium.
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bioRxiv - Neuroscience 2022Quote: ... 5.5% goat serum and 5.5% donkey serum and incubated overnight with a guinea pig polyclonal vGlut2 antibody (1:250, Millipore AB2251-1, RRID:AB_2665454). After primary antibody incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated for 1 hour at room temperature with a goat anti-mouse alkaline phosphatase-conjugated secondary antibody (dilution 1: 5000; Sigma-Aldrich, USA) and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2023Quote: ... and stained according to the immunohistochemistry protocol above (primary antibodies: anti-ZnT3, rabbit polyclonal, 1:500, Synaptic Systems; anti-puromycin 1:1000, EMD Millipore) and imaged on the Zeiss LSM 880 with Airyscan using a LD LCI Plan-Apochromat 25x/0.8 mm Korr DIC M27 and 1.8x zoom ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...