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Citations for Millipore Sigma :
3501 - 3544 of 3544 citations for HSPB8 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Genetics 2024Quote: ... Oviducts were dissected and oocytes from 2 animals were collected in 30 μl drops of HTF medium (EmbryoMax® Human Tubal Fluid Millipore, MR-070-D). Sperm was collected from 2 cauda epididymides in 50 μl drops of HTF medium ...
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bioRxiv - Microbiology 2024Quote: ... using 100 mg of human stool sample per 1 ml of PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma-Aldrich, St-Louis, MO, USA). The suspension was incubated for 10 min to allow large particles to settle ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365-375 of human β-actin) were obtained from Sigma (Madrid, Spain). Mouse monoclonal Anti-GOK/STIM1 antibody (Clone 44/GOK ...
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bioRxiv - Immunology 2024Quote: ... followed by washing with pre-warmed PBS and incubation for another 5 days in RPMI medium supplemented with 10% human AB serum (HS, Sigma-Aldrich, St. Louis, MO), 100□U/ml penicillin ...
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bioRxiv - Biochemistry 2023Quote: McArdle RH7777 rat hepatocytes (McA cells) stably expressing human apoB100[31] were grown in culture plates coated with type I collagen from calf skin (Sigma Aldrich, St. Louis, MO) as previously described[32] and cultured in growth media composed of Dulbecco’s modified – Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2024Quote: For podocyte stimulation the intact kidney was perfused through the arterial catheter with 250 µl of 10 µM human angiotensin 2 (Sigma-Aldrich, Darmstadt, Germany, A9525), dissolved in custom kidney buffer (10 mM NaHCO3 ...
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Developmental Biology 2023Quote: ... Cumulus oocyte complexes were harvested 14 h after hCG injection and placed in human tubal fluid (HTF) medium supplemented with 4 mg/ml bovine serum albumin (BSA, A3311; Sigma-Aldrich, St. Louis, MO) covered with paraffin oil (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... Then we added a series of two-fold dilutions of purified human SP-A (0-10 µg/ml, 100µl/well) in TBST containing either 5 mM CaCl2 (Sigma-Aldrich, Saint Louis, MO, USA) or 10 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: 3μm sections of formalin fixed and paraffin-embedded (FFPE) human carotid artery samples from the Munich Vascular Biobank25 were mounted on 0.1% poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) pre-coated SuperFrost Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... the cells were spun down and the supernatants were collected for analysis by Human T Cell 17-plex panel (Sigma-Aldrich, Saint Louis, Missouri). For NK cell stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Cumulus-oocyte complexes were placed in a 100-μL droplet of human tubal fluid medium supplemented with 4 mg/mL bovine serum albumin (BSA; A3311; Sigma Aldrich, St. Louis, MO) (Minami ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions: anti-human γH2AX Ser139 (EMD Millipore; JBW301; 1:200), goat anti-mouse IgG AF546 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 80 µg/mL human HDL (Millipore, LP3, MW 175,000-360,000) or LDL (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: βII-spectrin-KO CRISPR cell lines were generated as follows: a predesigned vector containing a human βII-spectrin-targeting gRNA and eCas9-2A-tGFP was purchased from Millipore Sigma (MISSION gRNA ID: HSP00000399630). 24 hours after transfection of this plasmid ...
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bioRxiv - Bioengineering 2020Quote: ... MSCs embedded in the PEG-NB gels were stained for α-SMA with FITC-conjugated mouse monoclonal anti-human α-SMA (F3777, Sigma; dilution 1:300), cellular extra domain-A (ED-A ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSC lines were dissociated to form embryoid bodies and cultured on mitomycin C-treated SNL murine fibroblast feeder cells in standard human embryonic stem cell culture (hESC) medium (DMEM/F12; Sigma Aldrich, St. Louis, MO, USA) containing 20% knockout serum replacement (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... codon optimized for expression in human cell lines and modified to include the human ER localization signal was cloned into the expression vector (Catalog Nr: OGS606-5U) under the EF1α promoter (Sigma Aldrich, St. Louis, MO, USA). Positive clones were verified by Sanger sequencing (Microsynth ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Immunology 2020Quote: ... plasma samples were incubated at a dilution of 1:100 and bound antibodies were detected with goat anti-human IgM/HRP (Sigma: cat. A6907, 1:6’000 dilution), goat anti-human IgG/HRP (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and grown as undifferentiated 3D cultures in 500 µL IntestiCult™ Organoid Growth Medium (OGM) Human (STEMCELL™ Technologies, cat# 06010) supplemented with 10 µM Y-27632 (Sigma-Aldrich, cat# Y0503). Undifferentiated monolayers were produced by plating single cell suspensions obtained from 7 days old highly dense 3D cultures ...
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... After washing, IgE antibodies were detected using biotinylated goat anti-human IgE (Vector, Burlingame, CA, USA) and streptavidin peroxidase (Sigma-Aldrich, St. Louis, MO, USA). The wells were then washed again with PBST buffer and incubated with 100 ul 3,3’,5,5’- Tetramethylbenzidine (Kirkegaard and Perry Laboratories ...
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bioRxiv - Developmental Biology 2023Quote: ... COCs were placed in a 100-μl droplet of human tubal fluid (HTF) medium supplemented with 4 mg/ml BSA (Sigma-Aldrich, St. Louis, MO, USA)28 ...
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bioRxiv - Developmental Biology 2023Quote: ... following intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (∼ 90 hours pre-collect) and 5 IU human chorionic gonadotropin (hCG; Sigma; ∼40-48 hours pre-collect) and mating with BDF1 male mice (Jackson 100006) ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Microbiology 2024Quote: ... blood mononuclear cells were isolated by density gradient centrifugation from buffy coats of human healthy donors (Ficoll-Paque Premium, Sigma Aldrich, St. Louis, MI, USA). Monocytes were purified from peripheral blood mononuclear cells by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Bioengineering 2020Quote: ... co-staining was performed using rabbit anti-perilipin A antibody as an adipocyte marker in combination with a mouse anti-human mitochondria (hMito) antibody conjugated with Cy3 (EMD Millipore, Burlington, USA; 1:100 dilution). hMito was used as an alternative to Ku80 based on the availability of antibodies compatible for co-staining ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK5 was silenced with human or mouse shRNAs purchased as pre-cloned in the pLKO.1-puro plasmid (MISSION® shRNA, Sigma-Aldrich, St Louis, MO, USA). Control shRNAs in the same plasmid were also purchased (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... the autoclaved peg lid was incubated in a flat-bottom 96-well plate with 200 µl of 100 µg/ml fibrinogen from human plasma (product number F3879, Sigma-Aldrich, in 0.9% NaCl or PBS) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were transfected as described above with empty or human SLFN14 expression plasmids and a plasmid encoding Hluc in the presence of RNA Pol III inhibitor (Sigma ML-60218, 40 and 20 uM) or tofacitinib (Sigma PZ0017 ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Cancer Biology 2023Quote: ... and HPCD-3BP were incubated at various concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, and 200 μM) with 90 μL of human serum (Sigma, Cat No. H3667; St. Louis, MO, USA) at 37 °C for up to 8 h ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.