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Citations for Millipore Sigma :
3451 - 3500 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Cell Biology 2024Quote: Proteins were resolved in 10% SDS PAGE and transferred to PVDF membrane (Millipore, Burlington ...
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bioRxiv - Immunology 2024Quote: ... The proteins on the gel were then transferred to PVDF membranes (Millipore, IPVH00010), which were blocked in 5% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a 0.45µm Immobilon-P Membrane (Millipore Sigma cat. IPVH00010) in ice-cold Tris-Glycine Buffer with 20% methanol in a transfer tank either overnight at 10V or 1 hour at 50V ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-glial fibrillary acidic protein (GFAP Cy3-conjugated Sigma-Aldrich, C9205, RRID:AB_476889), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were electrophoretically transferred to an Inmobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore) using the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using a 30 K Amicon Ultra-4 concentrator (Millipore) and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000). Membranes were developed using the ECL Prime Western Blotting System (Amersham Protran ...
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bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein eluate was further concentrated in a 50 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superose 6 Increase 10/300 GL (Cytiva ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated protein was isolated from the samples using phosphotungstic acid (PTA; Sigma-Aldrich) as described.9 ...
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bioRxiv - Microbiology 2024Quote: ... All proteins were concentrated using a BioMax concentrator (EMD Millipore, Burlington, MA, USA) followed by passage through a 0.2-μm Ultrafree-MC centrifugal filtration device (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... parasitica lysates using the OxyBlot Protein Oxidation Detection kit (Millipore, Billerica, MA, USA). Carbonylated proteins were derivatized with 2,4 Dinitrophenyl hydrazine (DNP ...
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bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal anti-HCoV-OC43 nucleocapsid protein (1:1000, Millipore Sigma, MAB9012). Secondary antibodies coupled to HRP were purchased from molecular probes and Jackson Immuno-research.
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with the enhanced chemiluminescent HRP substrate Crescendo Immobilon (Millipore). Analysis was performed in ImageJ ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated with Amicon® 3 kDa MWCO concentrator (Millipore, Burlington, MA) and injected into a 10/300 Superdex 75 size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 μg of protein was loaded into a 10 kDa centrifugal filter tube (Millipore), washed twice with 200 μl of UA buffer (8 M urea in 0.1 M Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved using SDS-PAGE and transferred onto Immobilon-FL PVDF membranes (Millipore). Membranes were blocked using Odyssey blocking buffer (Li-Cor ...
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bioRxiv - Cell Biology 2020Quote: ... protein translation was briefly inhibited by treatment with 20 mg/mL cycloheximide (CHX; Sigma) for 8 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Systems Biology 2020Quote: ... Particle-bound proteins were transferred to a filter plate (Millipore MultiScreen, 0.22 µm, MSGVN2250) and centrifuged (1000 g ...
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bioRxiv - Developmental Biology 2021Quote: ... 70µg total protein per sample was reduced with tris-2-carboxyethyl phosphine (TCEP, Sigma) for 1 hour at 60°C at a final concentration of 5mM ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). Membranes were blocked with 2% BSA in TBS tween20 0.1% and incubated in the presence of the primary and then secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Protein lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St Louis, MO) as per manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Millipore, Cork, Ireland), which were blocked with a 2:1 ratio of Odyssey blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of proteins was incubated with anti-HA affinity matrix (Sigma, #11815016001) to pull-down SMO-HA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced by 0.2 mM Isopropyl-ß-D-thiogalactopyranoside (IPTG; Sigma-Aldrich) and continued at 20°C for 20 h ...
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bioRxiv - Cell Biology 2020Quote: ... isolated heart mitochondria were solubilized with digitonin (Sigma Aldrich, 6g/g digitonin/protein ratio) and resolved on a 4-12% Bis-Tris polyacrylamide gradient gel (Genscript) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was incubated with Protein G-coupled magnetic beads (Sigma, MO, USA) that captured with anti-GFP for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Protein quantification was carried out using BCA (Bicinchoninic Acid, Sigma-Aldrich, St. Louis, MO) method taking bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were expressed in Sf9 insect cells and purified on M2 agarose (Sigma Aldrich) as described (J ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Molecular Biology 2022Quote: Crude protein extracts were mixed with 1% Sodium Dodecyl Sulphate (SDS; Sigma-Aldrich, USA) and boiled for 1 min ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Cell Biology 2022Quote: ... were coated with the following ECM proteins when noted: gelatin (Sigma Aldrich, catalog # G2500) (0.1% (w/v ...
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bioRxiv - Genetics 2022Quote: ... Immune complexes were collected by centrifugation and cleared using Protein A agarose beads (Millipore) and incubated for 1.5 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein extracts were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). Tubulin was used as a loading control (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated using a 10 kDa molecular weight cut-off concentrator (Millipore) and loaded onto a HiLoad 16/600 Superdex 75 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of Magna ChIP protein A magnetic beads (16–661, Millipore, Molsheim, France) were added for 1h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was concentrated with a 30-kDa molecular mass cut-off concentrator (Millipore) by centrifugation for 5 min at 2,500 x g ...
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bioRxiv - Developmental Biology 2022Quote: ... and separated proteins were transfer to polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Fisher Scientific). Total lane protein was then determined via Ponceau stain (0.1% Ponceau-S in 5% acetic acid ...