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Citations for Millipore Sigma :
3451 - 3500 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: HDX of transferrin was performed by diluting solid apo-human transferrin (Sigma-Aldrich to a concentration of 10 μM in equilibration buffer (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... a 100 μg/mL stock of human epidermal growth factor (hEGF; Sigma: E9644) was prepared in 0.22 μm filter-sterilized 10 mM acetic acid (Chem Lab ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10 % human serum (male AB, H6914-100ml Batch SLBT2873, Sigma-Aldrich), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: We prepared samples of four different iron compounds: transferrin (holo-transferrin human, Sigma), apo-transferrin (apo-transferrin human ...
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bioRxiv - Cell Biology 2023Quote: ... 5UI of hCG—human Chorionic Gonadotropin (CG10, Sigma-Aldrich, St. Louis, MI, USA) were applied to mice at 1pm on the third day of the protocol (46th hour after using PGMS) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human vinculin (mouse monoclonal, VIN-1, Sigma Aldrich, V9131; WB 1:1000), anti-human Hic-5 (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... These evaluations were performed by the commercial Human ELISA kits (Sigma-Aldrich, USA) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 ng of human urokinase-type PLG activator (uPA, Sigma-Aldrich) was added per well ...
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bioRxiv - Cell Biology 2023Quote: ReNcell VM immortalized human neural progenitor cells (hNPC) were purchased from EMD Millipore, initially derived from the ventral mesencephalon region of human fetal brains ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 50ng of esiRNA Human KCNH2 (Cat. EHU080951, Milipore Sigma) or siRNA Universal Negative Control per 5.0 x 104 hiPSC-CMs using Mission® siRNA Transfection Reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with 270ng of esiRNA Human GJA1 (Cat. EHU105621, Millipore Sigma) or siRNA Universal Negative Control (Cat ...
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bioRxiv - Pathology 2023Quote: ... the anti-human CD31 primary antibody (clone JC70, Cell Marque, Millipore Sigma, USA), and the detection with impact DAB (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... and 1% (v/v) human serum from platelet poor plasma (P2918; Sigma-Aldrich) and seeded into a T75 cell culture flask that was previously coated with 0.1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... 2 μm latex green beads were incubated with human IgM (I-8260, Sigma), then in rabbit anti-human IgM (Sigma 270A ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.15×106 of murine stromal cells expressing human delta like ligand 1 (Sigma) were combined with 7.5×103 of sorted and enriched KO-/- ...
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bioRxiv - Biochemistry 2023Quote: ... Zymosan A was opsonized by incubation with human serum (Sigma-Aldrich, no. H4522) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... human iPSCs were cultured on gelatin-coated dishes in differentiation medium (GMEM (Sigma) supplemented with 1mM sodium pyruvate ...
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bioRxiv - Cell Biology 2023Quote: ... MDM were cultured in RPMI 1640 medium supplemented with 10% human serum (Sigma). VSVDG pseudotyped HIVD1 GFP virus was produced by transfecting of 293T with GFPDencoding genome CSGW ...
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bioRxiv - Pathology 2023Quote: Human hepatoma cell lines were grown in Minimum Essential Medium (MEM, Sigma Aldrich) supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2023Quote: ... The shRNAs targeting human MARCHF8 were ordered from Sigma-Aldrich (St. Louis, MO). The sgRNAs targeting Marchf8 were designed using the web-based software ChopChop (chopchop.cbu.uib.no ...
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bioRxiv - Cell Biology 2023Quote: ... glass coverslips were coated with 10 µg/mL human plasma fibronectin (FC010, Millipore) overnight at 37◦C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected with 270ng of esiRNA Human KCNJ2 (Cat. EHU019661, Millipore Sigma) or non-targeting control (NTC ...
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bioRxiv - Immunology 2024Quote: ... MILLIPLEX Map Human cytokine/chemokine Magnetic Bead Panel – Premixed 41 Plex (Millipore Sigma), run on a MAGPIX® xMAP instrument according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Human spinal cord samples were homogenized with a dounce homogenizer (Sigma Aldrich, D8938) in 1mL nuclei lysis buffer using the Nuclei Pure Prep Nuclei Isolation Kit (Sigma Adlrich ...
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bioRxiv - Cell Biology 2024Quote: ... primary human skeletal muscle cells were seeded and differentiated on laminin (Sigma Aldrich) coated 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... We obtained the antibodies through commercial suppliers: IgA from human serum (Sigma-Aldrich), IgM from human serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: Fresh or cryopreserved primary adult human hepatocytes (BeCytes Biotechnologies, BiolVT or Sigma-Aldrich) were resuspended in hepatocyte spheroid medium (HSM) ...
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bioRxiv - Immunology 2024Quote: ... coated with human placental type IV collagen (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured using Ex Plus in both the basolateral and apical compartments for 1 week ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips with C3bi-coated beads were incubated with rabbit anti-human IgM (Sigma) at 1:250 ...
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bioRxiv - Microbiology 2024Quote: Purified human PMNs were incubated with phorbol 12-myristate 13-acetate (PMA) (Sigma) 100nM for 4h ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G and Protein A Dynabeads (1:1 mixed) were blocked with 1mg/ml denatured yeast tRNA (Sigma-Aldrich, R5636) and 1mg/ml of BSA (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...