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Citations for Millipore Sigma :
3451 - 3500 of 4323 citations for Recombinant Human BMPR2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody against pTBC1D1 Ser231/237 (07-2268, mouse/human) was from Millipore (Burlington, MA, USA) and the antibodies against TBC1D1 (ab229504) ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Cell Biology 2023Quote: ... human HT1080 fibrosarcoma cells (ACC315; DSMZ Braunschweig) were cultured in Dulbecco’s modified Eagle medium (DMEM; Sigma Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Human pancreatic cancer cells (BxPC-3; ATCC, CRL-1687) were cultured in RPMI-1640 medium (Sigma‒Aldrich) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: Human U937 monocytic cells were propagated and differentiated into macrophages using phorbol myristate acetate (PMA, Sigma-Aldrich) as previously described (Tanner et al. ...
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bioRxiv - Molecular Biology 2024Quote: Human neuroblastoma SH-SY5Y cells (ATCC CRL-2266) were cultured in Dulbecco’s modified Eagle’s Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Physiology 2024Quote: ... followed 48 h later by injection of 5 IU of human chorionic gonadotrophin (hCG) (Sigma Cat.# CG10). Thirteen to fourteen hours after hCG injection ...
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bioRxiv - Evolutionary Biology 2024Quote: PC-9 are human adenocarcinoma cells derived from undifferentiated lung tissue were obtained from Sigma (Sigma, USA). PC-9 cells were cultured in RPMI-1640 medium supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Antibody bound to GP was then detected with anti-human antibody conjugated to HRP (EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Cell Biology 2020Quote: Senescence in normal human fibroblasts was induced using five treatments with 30 μM tert-butyl hydroperoxide (tBHP, Sigma) for 5 consecutive days (1 h/day ...
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bioRxiv - Immunology 2021Quote: ... were added to either tissue culture-treated plates coated with 10 μg/ml human plasma fibronectin (Sigma-Aldrich) or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... mice underwent the full superovulation procedure with an intraperitoneal injection of 10 IU human chronic gonadotropin (hCG, Sigma) 48 hours after PMSG administration ...
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bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... human HT-1080 fibrosarcoma cells (CCL-121; ATCC) were maintained using Eagle’s Minimum Essential Medium (EMEM; Millipore Sigma) supplemented with 2 mM L-glutamine and containing 10% (vol/vol ...
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bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no ...
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bioRxiv - Biochemistry 2021Quote: ... reactions were then incubated in the presence of 50 μg/mL (~0.1 units) human salivary α-amylase (Sigma) or 1.5 M hydroxylamine for a further 60 min at 37°C ...
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bioRxiv - Genetics 2021Quote: ... AG1-0 cells were passaged according to the product manual for PluriSTEM Human ES/iPS Medium (Millipore Sigma), which is feeder-free and utilizes Dispase II (Millipore Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse monoclonal antibodies against human LMα3 (clone CL3112) and mouse IgG (both Sigma-Aldrich, St. Louis, Missouri, USA) were used at 0.5 μg mL−1.
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in Human IntestiCult™ Organoid Growth Medium (StemCell) supplemented with 10 mmol/L Y-27632 (Sigma), 5 mmol/L CHIR99021 (ReproCell ...
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bioRxiv - Neuroscience 2021Quote: ... the eye cup was treated for ∼15 minutes with human plasmin (∼50 μg/mL, Sigma or Haematologic Technologies) to aid vitreous removal ...
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bioRxiv - Cell Biology 2019Quote: ... either directly or after coating with 10 µg/ml human plasma fibronectin (cat no. FC010; Millipore, Billerica, MA), as noted in the figure legend.
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bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated with the respective primary antibody (rabbit anti human, anti-ANK1 or anti-EPB41, Sigma-Aldrich) overnight ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...
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bioRxiv - Neuroscience 2020Quote: Chromatin for ChIP was prepared from immortalized human microglia using the Imprint® Chromatin Immunoprecipitation kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Human colon cancer cell lines HCT116 TP53+/+ and HCT116 TP53-/- were grown in McCoy’s 5A Medium (Sigma, M9309) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibody was detected with an anti-human antibody conjugated to horseradish peroxidase (HRP; EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... α-taxilin (NM_175852.4) and its truncated segments were amplified from human cDNA and cloned into pcDNA3.1(+) (Sigma-Aldrich). Full-length α-taxilin and γ-taxilin were also cloned into p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa D2 parental control cells were transfected with 300 ng/ml human VRK1 MISSION® esiRNA (EHU119541, Sigma) using HiPerFect Transfection Reagent (301707 ...
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... Then the inoculum was aspirated and DMEM supplemented with 5% NBCS and 7.5 µg/mL human immunoglobulin (Sigma) was added ...
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bioRxiv - Immunology 2021Quote: ... Serum IgG subclasses were tested with isotype-specific murine anti-human IgG subclasses (Sigma-Aldrich, S:t Louis, MA).
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma), 100U/mL P/S ...
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bioRxiv - Microbiology 2021Quote: ... resolved by SDS–PAGE and analysed by immunoblotting using primary antibodies specific for human DDX42 (HPA023571, Sigma-Aldrich), Flag (M2 ...
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bioRxiv - Microbiology 2021Quote: ... Human HeLa cells (ATCC CCL-2) were maintained in Minimum Essential Medium Eagle (MEM) (Sigma-Aldrich, Taufkirchen, Germany), 7% FBS and 1% MEM non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: Human THP-1 monocytes were differentiated into macrophages by 24 hr incubation with 100 nM PMA (Sigma-Aldrich) in complete RPMI medium at 1.6 × 105 cells/well in 12-well plates ...
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bioRxiv - Bioengineering 2022Quote: ... suspended in a solution prepared in the following order: 30 μL of human male AB serum (Sigma-Aldrich), 60 μL human plasma fibronectin (EMD Millipore) ...
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bioRxiv - Bioengineering 2022Quote: ... KCs were cultured and serially passaged into 7 μg/cm2 human collagen IV-coated plates (#C7521; Sigma-Aldrich). All cell sources were obtained at Yale-New Haven Hospital under protocols approved by the Yale University Institutional Review Board including ...
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bioRxiv - Biophysics 2022Quote: ... for mouse ILC2s and human peripheral ILC2s was supplemented with MEM nonessential amino acid solution (M7145, Sigma-Aldrich), 10 mM HEPES buffer (H3537 ...
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bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...