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Citations for Millipore Sigma :
3451 - 3500 of 3780 citations for IGSF11 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Multiplex bead assays for plasma samples were performed in duplicate for TNF from the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma). Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cancer Biology 2019Quote: ... Human ovarian cancer cell line OV90 (ATCC CRL-11732) was cultured in 1:1 volume of MCDB 105 medium (Sigma-Aldrich) and Medium 199 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... All other cell lines were grown in Dulbecco`s modified Eagle medium (DMEM) (Fisher, UK) supplemented with 2% human serum (Sigma, UK) and maintained in an humidified sterile incubator with 5% CO2 at 37°C.
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... Females were superovulated at 6-8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HNECs were then seeded on 12 mm transwell inserts with 0.4 μm pores (Costar 3460) coated with human placental collagen (Sigma C7521-10MG) at a density of 203,000-333,000 cells per well and maintained in PneumaCult -Ex Plus Medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... The serum-free medium –SerM was prepared as described previously by replacing 10% v/v heat-inactivated human serum with oleic and palmitic acid (Sigma Aldrich) in a final concentration of 30 µM each ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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bioRxiv - Cell Biology 2021Quote: Human leukaemia cells HL-60 and breast cancer cell line MCF-7 were cultured in RPMI and DMEM media (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2021Quote: ... falciparum parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Bioengineering 2021Quote: ... or type I collagen microchannels coated overnight with 50 μg mL−1 human placental collagen IV (Cat. no. C5533, Sigma-Aldrich) and 25 μg mL−1 fibronectin from human plasma (Cat ...
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bioRxiv - Developmental Biology 2020Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 μg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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bioRxiv - Cancer Biology 2021Quote: Selective depletion of the myeloid cell compartment in human neocortical slices was performed by supplementing the growth medium with 11 μmol of Clodronate (Sigma, D4434) for 72h at 37ºC ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Biochemistry 2021Quote: ... was cultivated in fresh human erythrocytes (either A⁺ or O+) in RPMI-1640 culture medium supplemented with 25 mM HEPES (pH 7.5, Sigma Aldrich, USA), 0.2 mM hypoxanthine (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... asexual blood stage cultures were maintained at 0.75-5% parasitaemia and 4% haematocrit using O+ or A+ human erythrocytes (NHS National Blood Service) supplemented with 30 units/ml heparin (Sigma-Aldrich). Culture medium was prepared from RPMI-1640 with 25mM HEPES (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... containing a cDNA sequence of WT human CAV1 codon optimized for E.coli expression and cloned into a pET20 vector (Novagen No. 69739) using Nde I and Xho I restriction sites ...
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bioRxiv - Genomics 2020Quote: ... of pregnant mare’s serum gonadotrophin (PMSG) (USBiological Life Sciences G8575A) and 5 IU of human chorionic gonadotrophin (hCG) (Sigma-Aldrich C1063) 46-48 h later ...
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bioRxiv - Immunology 2020Quote: Human bronchial epithelial cells (BEAS-2B) were obtained (ATCC, Manassas, VA, USA) and maintained in HAM’s F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD ...
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bioRxiv - Immunology 2020Quote: ... The THP1-Dual and THP1-Dual KO (IRF3/TBK1/MyD) cells (human lung monocytes) were cultured in RPMI 1640 (Sigma-Aldrich) medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Microbiology 2021Quote: ... and then seeded at a 1:4 dome-to-well ratio in a 96-well plate coated in 100 µL of 34 µg/mL human collagen IV (Sigma C5533). Cells were fed every two days and were used for experiments after they were fully confluent (4-5 days) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Bioengineering 2021Quote: Day-21 TSC2+/+ organoids were incubated in Advanced RPMI + 1X L-GlutaMAX with 50ng/ml human IL1-β (Sigma-Aldrich # H6291) for 96hs ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into mouse hypothalamus Pomc neuronal cells (mHypoA-POMC/GFP-1 from Sanbio #CLU500) or human astrocytes (CCF-STTG, ATCC) grown in 96-well plates of 132 using X-tremeGene (Sigma, 6366244001) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Genetics 2022Quote: ... then dissociated again using “program C” and transferred through a 100 μm cell strainer into 20mL RPMI containing 10% human AB serum (Sigma Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: Serum samples were diluted 1:2 in serum matrix for analysis using the Milliplex Non-Human Primate Magnetic Bead Panel as per the manufacturer’s instructions (Millipore, Burlington, MA). Concentrations for G-CSF ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Cell Biology 2022Quote: ... medium was exchanged with 900 μl of fresh growth medium before cells were transfected with 40 nM of esiRNA against human FXR (MISSION esiRNA, Sigma, EHU133161). EsiRNA was diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Cancer Biology 2022Quote: Human glioma cell line U-87MG (ECACC Product number 89081402, Salisbury, Wiltshire, UK) was maintained in complete medium MEM (Sigma, USA), supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 Mio cells per mL were stimulated in cRPMI (see Methods – primary human material and cell lines) with 20 ng/mL PMA (Sigma Aldrich) for 1h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from approximately 1,000 differentiated aggregates (∼1,000 cells per aggregate) or approximately 35 mg of frozen human pancreas using a nuclei permeabilization buffer [0.1% Triton X-100 (Sigma-Aldrich, T8787), 1X Pierce Protease Inhibitor (Fischer ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were transferred into a 100-μL droplet of human tubal fluid (HTF) medium supplemented with 4 mg/mL bovine serum albumin (BSA; Sigma-Aldrich) (Minami et al. ...
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bioRxiv - Immunology 2022Quote: ... T cells were cultured with X-VIVO 15 Cell Medium (Cultek, BE02-060Q) supplemented with 5% AB human serum (Sigma, H4522), penicillin-streptomycin (100 mg/mL) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson Immuno Research 109-036-088 109-035-129 and Sigma A0295) in blocking buffer at a 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...