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Citations for Millipore Sigma :
3451 - 3500 of 3706 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Samples of gB-SE or gD-SE immobilized on Strep-Tactin Sepharose beads were incubated with 1 mL of diluted human γ-globulins (G4386; Sigma) (0.082 and 3.04 mg/mL in medium 199 containing 1% FCS and ADCC assay buffer for neutralization assays and ADCC assays ...
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bioRxiv - Microbiology 2022Quote: Human foreskin fibroblast (HFF; ATCC® designation SCRC-1041 ™) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Sigma, D6546) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C and 5% CO2. Human BLaER1 cells (a kind gift of Thomas Graf) (Rapino et al. 2013) cells were grown in RPMI (Sigma-Aldrich) supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: The MISSION® pLKO.1-puro Non-Target shRNA (scr) and the shRNA against the UTR of human ZIP11 gene (5’-TCCTGATTGACTCTGATTATA-3’, Cat. TRCN0000434903) were from Sigma-Aldrich. The mammalian gene expression vector pLV[Exp]-EGFP/Neo-Ef1A (pLV ...
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bioRxiv - Neuroscience 2023Quote: ... and Aβ 1-42 using the Milliplex® MAP Human Amyloid Beta and Tau Multiplex Assay kit (Millipore Sigma HNABTMAG-68K). All kits were read on a MAGPIX® system (Luminex ...
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bioRxiv - Immunology 2023Quote: ... 100,000 T84 cells per well were seeded on glass bottom 8-well chamber slides coated with 2.5% human collagen (Sigma #C5533-5MG) diluted in water ...
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bioRxiv - Microbiology 2023Quote: ... Presence or absence of a ZCCHC3 specific band with each clone was determined by western blotting using an anti-human ZCCHC3 antibody (SAB1408147, Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human CD56 Positive Selection Kit II) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Despite the successful reprogramming of a human neonatal foreskin or adult skin (normal) fibroblast line purchased from Sigma (Cat 106-05A), multiple attempts to reprogram the Nigerian fibroblasts failed for reasons that are not entirely clear ...
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bioRxiv - Cell Biology 2023Quote: ... coating for 5 min at 22 °C protected from light with sterile filtered DOPA in pH 10 [10 mM] Tris buffer DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 µg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... were superovulated by injecting them with pregnant mare serum gonadotropin (5 IU, Prospec) and human chorionic gonadotropin hormone (5 IU, Sigma-Aldrich) intraperitoneally at 48-h intervals ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.75 IU/kg of human insulin (Actrapid 100 IU/mL, Novo Nordisk) for insulin tolerance tests (ITT) or 2 g/kg pyruvate (Sigma-Aldrich) for pyruvate tolerance tests (PTT ...
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bioRxiv - Bioengineering 2023Quote: ... Human mammary epithelial cell line (MCF10A) was cultured in DMEM/F12 (Cytivia: SH30023.01) with 5% horse serum (Sigma Aldrich: H1138-500ML), 0.2% Normocin ...
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bioRxiv - Biophysics 2023Quote: Human Serum from human male AB plasma (H6914, 1st lot SLCH8785, 2nd lot SLCJ3635) and (H4522, lot SLCK9619) were purchased from Sigma-Aldrich. Serum samples from healthy individuals and cancer patients ...
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bioRxiv - Cell Biology 2022Quote: Ibidi µ-Slide I slides (#80106, IbiTreat) were incubated with 10 μg/mL fibronectin (from human plasma, #2006, Sigma-Aldrich Inc.) in PBS at room temperature for 1.5 hr ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... the caudal epididymis was dissected and the epididymal sperm was collected on a needle and capacitated in EmbryoMax Human Tubal Fluid media (HTF, EMD Millipore) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: Serum autoantibodies were quantified using the Luminex xMAP platform (Luminex) with the MILLIPLEX MAP Human Autoimmune Autoantibody Panel (HAIAB-10K, Millipore Sigma) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
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bioRxiv - Cell Biology 2023Quote: Round 25mm coverslips were squeaky-cleaned (56) and plasma cleaned before coating with 20µg/mL Human Plasma Fibronectin (EMD Millipore #FC010) at 37C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were harvested and maintained in T cell medium containing Roswell Park Memorial Institute 1640 Medium (RPMI) supplemented with 100 U/ml Penicillin-100ug/ml Streptomycin and 10% human serum (HS, Sigma-Aldrich), IL-7 (ImmunoTools ...
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bioRxiv - Molecular Biology 2023Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Cell Biology 2023Quote: IMR-90 normal human fibroblasts (ATCC, CCL-186) and HEK293T cells were cultured in DMEM (Sigma-Aldrich, D5796; Wako, 044-29765) supplemented with 10% FBS (BioWest ...
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bioRxiv - Genetics 2023Quote: ... Control female mice were injected with 5 U of pregnant mare serum gonadotropin (PMSG, nor272-Prospecbio, Israel) and 46 h later injected with 5 U of human chorionic gonadotropin (HCG, C1063 Sigma-Aldrich) and placed into the home cage of the male to mate overnight ...
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bioRxiv - Bioengineering 2023Quote: ... hydrogel used within the arrays was previously developed to mimic the material properties of human mucus.29 A solution of 4% porcine gastric mucins (PGM; Sigma Aldrich; mucin from porcine stomach ...
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bioRxiv - Immunology 2023Quote: ... and maintained at 37°C with 5% CO2 in complete RPMI in the presence of 5% human AB serum (Sigma-Aldrich) for differentiation into MDMs for 10-14 day (Pierini et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Cell Biology 2024Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... 5 U thrombin increments were added to microcentrifuge tubes containing 50 µl of human citrated plasma (Sigma Cat No. P9523-1ML), the time to clot formation was recorded ...
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bioRxiv - Bioengineering 2024Quote: To test the micromechanical properties of meniscal cells and newly synthesized PCM, 35-mm petri dish (351008, Life Sciences) was treated with 20 µg/mL human fibronectin (10838039001, Sigma-Aldrich) for 12 h prior to seeding meniscal cells with an initial density of 5,000 cells per dish ...
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bioRxiv - Microbiology 2024Quote: ... by human neutrophils was measured over a time of 30 minutes by monitoring luminol-amplified chemiluminescence using 282 μM luminol (Sigma Aldrich). Neutrophils were stimulated either with negative control (Hank Balanced Salt Solution ...
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bioRxiv - Cell Biology 2024Quote: ... The shRNA plasmids were obtained from the human genome-wide TRC shRNA library (purchased from Sigma-Aldrich, USA, Catalog no. SH0411), available at shRNA resource centre ...
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bioRxiv - Neuroscience 2024Quote: Proteins from human cerebral cortex and whole mouse brain were extracted using RIPA buffer (MilliporeSigma) with Protease Inhibitor cocktail (Sigma P8340) as previously described [20] ...
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bioRxiv - Physiology 2024Quote: ... and 120 min after intraperitoneal injection of 1g/kg glucose (glucose tolerance test) or 0.5 U/kg of recombinant human insulin (Sigma Cat.# I6634) (insulin tolerance test).
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bioRxiv - Cell Biology 2024Quote: ... the media were switched into Phase II erythroid differentiation media consisting of Stem Span SFEMII supplemented with 3% normal human serum (Sigma-Aldrich), 3 IU/ml erythropoietin (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were adjusted to 5 x 106 bacteria per 50 μL in PBS then treated with indicated OspC MAbs (1, 2, 3, 10, 20, 30 μg/mL) in the presence or absence of 20% human complement (Sigma-Aldrich) for 16 or 24 h ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Cell Biology 2020Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 120 μg/mL human HDL (Sigma-Aldrich, L1567) for 6 h ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Immunology 2021Quote: ... fresh living donor biopsies or cells remaining following 10X cell capture for sequencing were resuspended in 90% human serum (Sigma, cat# H4522) and 10% DMSO for cryopreservation and cooled to -80°C in a Mr.Frosty (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Bioengineering 2019Quote: ... human skeletal muscle samples were minced and then dissociated into a single cell slurry with Clostridium histolyticum collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Bioengineering 2019Quote: ... were maintained as monolayer cultures in flasks pre-coated with 60 μg/mL solution of human placental collagen type IV (Sigma Aldrich, MO) and grown in bronchial epithelial growth medium (BEGM ...