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Citations for Millipore Sigma :
3451 - 3500 of 3774 citations for FGF 21 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... DOPA coated PA-gels were washed 2x with sterile PBS and ECM functionalized with 5 μg/mL human fibronectin (Millipore, FC010) in sterile PBS 1 h at 37 °C to generate an expected fibronectin coating density of 6 μM/cm2.
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bioRxiv - Cancer Biology 2021Quote: Selective depletion of the myeloid cell compartment in human neocortical slices was performed by supplementing the growth medium with 11 μmol of Clodronate (Sigma, D4434) for 72h at 37ºC ...
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bioRxiv - Biochemistry 2021Quote: ... Rabbit anti-SNAP-25 polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of human SNAP-25 (Sigma S9684), was used as a positive control to detect both cleaved and intact SNAP-25 ...
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bioRxiv - Biochemistry 2021Quote: ... was cultivated in fresh human erythrocytes (either A⁺ or O+) in RPMI-1640 culture medium supplemented with 25 mM HEPES (pH 7.5, Sigma Aldrich, USA), 0.2 mM hypoxanthine (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... asexual blood stage cultures were maintained at 0.75-5% parasitaemia and 4% haematocrit using O+ or A+ human erythrocytes (NHS National Blood Service) supplemented with 30 units/ml heparin (Sigma-Aldrich). Culture medium was prepared from RPMI-1640 with 25mM HEPES (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4-weeks old female mice were injected intraperitoneally with 5IU Pregnant Mare Serum Gonadotropin (PMSG, Prospec) followed by 5 IU human Chorionic Gonadotropin (hCG, Sigma-Aldrich) 46-48 hours later ...
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bioRxiv - Cell Biology 2020Quote: ... containing a cDNA sequence of WT human CAV1 codon optimized for E.coli expression and cloned into a pET20 vector (Novagen No. 69739) using Nde I and Xho I restriction sites ...
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bioRxiv - Genomics 2020Quote: ... of pregnant mare’s serum gonadotrophin (PMSG) (USBiological Life Sciences G8575A) and 5 IU of human chorionic gonadotrophin (hCG) (Sigma-Aldrich C1063) 46-48 h later ...
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK) 293T cells and human non-small-cell lung cancer (NSCLC) A549 cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: Human bronchial epithelial cells (BEAS-2B) were obtained (ATCC, Manassas, VA, USA) and maintained in HAM’s F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD ...
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bioRxiv - Immunology 2020Quote: ... The THP1-Dual and THP1-Dual KO (IRF3/TBK1/MyD) cells (human lung monocytes) were cultured in RPMI 1640 (Sigma-Aldrich) medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Microbiology 2021Quote: ... and then seeded at a 1:4 dome-to-well ratio in a 96-well plate coated in 100 µL of 34 µg/mL human collagen IV (Sigma C5533). Cells were fed every two days and were used for experiments after they were fully confluent (4-5 days) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, A8592, 1:5,000 dilution) and TMB substrate ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into mouse hypothalamus Pomc neuronal cells (mHypoA-POMC/GFP-1 from Sanbio #CLU500) or human astrocytes (CCF-STTG, ATCC) grown in 96-well plates of 132 using X-tremeGene (Sigma, 6366244001) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Females were super-ovulated at 6–8 weeks with 0.1 ml CARD HyperOva (Cosmo Bio Co., Cat. No. KYD-010-EX) and 5 IU hCG (Human chorionic gonadotrophin, Sigma-Aldrich) at intervals of 48 hours ...
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bioRxiv - Genetics 2022Quote: ... then dissociated again using “program C” and transferred through a 100 μm cell strainer into 20mL RPMI containing 10% human AB serum (Sigma Aldrich). Next ...
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bioRxiv - Immunology 2022Quote: Serum samples were diluted 1:2 in serum matrix for analysis using the Milliplex Non-Human Primate Magnetic Bead Panel as per the manufacturer’s instructions (Millipore, Burlington, MA). Concentrations for G-CSF ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Cell Biology 2022Quote: ... medium was exchanged with 900 μl of fresh growth medium before cells were transfected with 40 nM of esiRNA against human FXR (MISSION esiRNA, Sigma, EHU133161). EsiRNA was diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Cancer Biology 2022Quote: Human glioma cell line U-87MG (ECACC Product number 89081402, Salisbury, Wiltshire, UK) was maintained in complete medium MEM (Sigma, USA), supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 Mio cells per mL were stimulated in cRPMI (see Methods – primary human material and cell lines) with 20 ng/mL PMA (Sigma Aldrich) for 1h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Cell Biology 2022Quote: Nuclei were isolated from approximately 1,000 differentiated aggregates (∼1,000 cells per aggregate) or approximately 35 mg of frozen human pancreas using a nuclei permeabilization buffer [0.1% Triton X-100 (Sigma-Aldrich, T8787), 1X Pierce Protease Inhibitor (Fischer ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were transferred into a 100-μL droplet of human tubal fluid (HTF) medium supplemented with 4 mg/mL bovine serum albumin (BSA; Sigma-Aldrich) (Minami et al. ...
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bioRxiv - Immunology 2022Quote: ... T cells were cultured with X-VIVO 15 Cell Medium (Cultek, BE02-060Q) supplemented with 5% AB human serum (Sigma, H4522), penicillin-streptomycin (100 mg/mL) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson Immuno Research 109-036-088 109-035-129 and Sigma A0295) in blocking buffer at a 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Neuroscience 2022Quote: ... Immunofluorescence was performed using an automatic liquid handling dispenser (Personal Pipetter, ApricotDesigns) using Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543) and Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
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bioRxiv - Cell Biology 2022Quote: Transient knockdown of human ANXA1 or LGN in MCF-10A cells was achieved by transfection of MISSION® Predesigned siRNAs from Sigma. The following products were used ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Microbiology 2024Quote: The human monocytic cell line U937 (ATCC CRL-1593.2) was obtained from ATCC and primary human peripheral monocytic cells (PBMCs) were purchased from Sigma (HUMANPBMC-0002644). U937 cells were cultured in RPMI medium (Genesee ...
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bioRxiv - Microbiology 2024Quote: ... Primary human PBMC were differentiated into macrophages (hMDMs) after culturing for 7 days with RPMI supplemented with 20% heat-inactivated human serum (Sigma-H4522).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were transferred to a new dish on ice and minced into ∼1mm3 pieces followed by enzymatic digestion using a cocktail for human tissues composed of Collagenase A (10103578001, Sigma Aldrich), Collagenase IV (NC9836075 ...
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bioRxiv - Molecular Biology 2024Quote: ... Spinfection plates were pre-coated using 500 uL of PBS with 10 uG/mL of purified human plasma fibronectin (Sigma-Aldrich) and incubated at 37°C several hours prior to transduction ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Cell Biology 2024Quote: ... U-2 OS human cell line (ATCC HTB-96) was transfected with the GFP-tau vector using the X-tremeGENE HP reagent (Sigma Aldrich) and then selected with 200 µg/mL zeocin ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Bioengineering 2024Quote: ... Rat tail collagen type I solution in 0.6% acetic acid (First Link, UK) was combined with human AB serum (HS; Sigma-Aldrich, H4522), 10x DMEM ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... The tissues were cryopreserved in cryo-medium (40% PBS; 50% Human Serum, Sigma-Aldrich, St. Louis, MO, USA; and 10% DMSO) and stored at -150 °C for further analysis.
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bioRxiv - Cell Biology 2024Quote: ... they were covered with a 100 µl drop of a solution of 20 µg/ml fibronectin (fibronectin from human plasma, Sigma Aldrich) and 15 µg/ml fibrinogen-Alexa488 conjugate (F13191 ...