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Citations for Millipore Sigma :
3451 - 3500 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Immunology 2020Quote: ... 1 × AA and 50 U/ml IL-2 (Sigma-Aldrich; MO, USA), were treated in triplicate respectively with D+T NP or D+T solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Lyophilized cytokines (TNF-α, IL-1α, C1q) were obtained from Sigma-Aldrich, resuspended in molecular biology grade water to make stock solutions ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Cell Biology 2024Quote: ... interleukin 1b (recombinant murine Il-1b, Sigma-Aldrich, #I5271, 10 ng/mL) and interferone γ (recombinant mouse INFγ ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies and antisera and dilutions were used: mouse anti-tubulin antibody (1:250, Millipore Sigma, cat# T5168); mouse anti-CSP (clone 2A10 ...
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bioRxiv - Neuroscience 2022Quote: ... in primary antibodies for 24 hours at 4°C [Primary antibodies: Mouse anti-tyrosine hydroxylase (TH) clone LNC1 (1:500; Millipore) and rabbit anti-vesicular monoamine transporter 2 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes were subsequently incubated overnight at 4 °C with primary antibodies (1:2,000 monoclonal mouse anti-TRPA1 antibody, Sigma-Aldrich, and 1:3,000 MF20 mouse anti-myosin heavy chain antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were loaded and run on SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with antiKv2.1 antibody (K89/34, NeuroMab) and anti-α tubulin antibody (Sigma). The blots were detected using chemiluminescence reagent on a Bio-Rad Chemidoc MP and Image J was used for quantification of detected bands.
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-α-tubulin primary antibody as well as secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Sigma-Aldrich. AlexaFluor-labelled secondary antibodies were obtained from ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with primary antibody overnight at 4 °C [1:500 goat anti-RNA polymerase II antibody (PLA0292, Sigma) with 1:500 rabbit anti-PCNA antibody (PLA0079 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... In this case the blot was incubated with the IcdH primary antibody at room temperature for 2 hours and with the secondary antibody (anti-rabbit HRP, Sigma) was done at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the presence of the viruses was revealed by immunostaining in infected cells with a polyclonal rabbit antibody that recognizes the MOPV Z protein (Agrobio, France) and a phosphatase alkaline-conjugated polyclonal goat anti-rabbit antibody (Sigma) and 1-Step NBT/BCIP substrate (Thermo Fisher scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... similarly performed as in co-immunoprecipitation except that the primary antibody used is anti-PCNA antibody (cat#SAB2108448; Sigma-Aldrich) in 1:750 dilutions.
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bioRxiv - Cell Biology 2019Quote: ... Bound primary antibodies were detected using peroxidase-coupled secondary anti-rabbit antibody (7074, Cell signalling) and enhanced chemiluminescence (WBLUF0500, Millipore). Blots were exposed digitally using the ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Immunology 2020Quote: ... Immunofluorescence staining using a goat anti-LYVE1 antibody (R&D) followed by incubation with an Alexa647-conjugated secondary antibody together with Hoechst33342 (Sigma) was performed on 7 µm sections without acetone fixation ...
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bioRxiv - Neuroscience 2021Quote: ... IHC-1:250, no. MAB5374), and Anti-polyglutamine (poly-Q) antibody (WB-1:5000, no. P1874) antibodies were obtained from Millipore-Sigma ...
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bioRxiv - Physiology 2020Quote: ... Brains were then preincubated in 5% normal goat serum in PBT for 3 hours at room temperature with gentle agitation followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS twice and then incubated in antibody solution (2% NGS, 0.2% Triton-X-100, PBS) with anti-PSD-95 antibody (Millipore, MABN68) for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for ChIP analysis: Mouse anti-RNA polymerase II antibody clone CTD4H8 (Merck Millipore, 05-623), Rabbit anti-NF-kB p65 antibody clone D14E12 (Cell Signalling ...
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bioRxiv - Plant Biology 2024Quote: ... and labelled with the secondary antibody (diluted 1:50 in 0.1% BSA in PBS, Anti-Rat-IgG – Gold antibody; Sigma, USA) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated overnight in primary antibody Anti-Tyrosine Hydroxylase Antibody from rabbit (1:500, cat# AB152, Sigma Aldrich) diluted in blocking solution at 4 °C ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... followed by TBS-Tx (2 × 5 min) and incubated with primary antibody (Rat anti-muscarinic M2 monoclonal antibody, unconjugated, clone m2-2-b3, 1:750, Millipore Cat ...
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bioRxiv - Neuroscience 2023Quote: ... Some of the UMB1465 samples were additionally stained with the following antibodies: two samples with anti-NEUN antibody (Millipore MAB377) for neuron sorting ...
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bioRxiv - Neuroscience 2023Quote: ... then blocked in 5% normal donkey serum for 1 hour prior to incubation in primary antibody (anti-GFAP primary antibody, 1:10,000, G9269, Sigma-Aldrich or anti-Iba1 primary antibody 1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were developed by chemiluminescence following incubation with secondary HRP-conjugated antibodies (1:2000) and an HRP-conjugated anti-β-actin antibody (1:40.000, Sigma) was used as loading control ...
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bioRxiv - Cancer Biology 2023Quote: Antibodies used in this study are as follows: mouse monoclonal anti-β-tubulin antibody (Sigma-Aldrich, Cat# T4026, 1:5000), mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used in this study were: polyclonal rabbit anti-human HINT1 antibody (1:1000, Sigma, San Luis, MO, USA), and to demonstrate equal loading mouse monoclonal anti-β-actin antibody (1:5000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling with a rat anti-phospho- histone H3 antibody (polyclonal rat antibody against phosphorylated Ser28 histone H3, Sigma- Aldrich, H9908) was carried out following the previously outlined techniques ...
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bioRxiv - Cell Biology 2023Quote: ... the cover slips were incubated with 150 μL of primary antibody (1:50 anti-FLAG M2 antibody, Sigma-Aldrich F1804) diluted in blocking solution overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated 1 hour at 25°C with a second antibody (anti-mouse HRP conjugated antibody; Sigma) diluted 1:10,000 in PBS-Tween ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with Odyssey blocking buffer (Li-Cor, Lincoln, NE) prior to incubation with appropriate antibodies (anti-DNMT-1,-3A,-3B antibody, 1:1000, Sigma; anti-DCTPP1 antibody ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...