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Citations for Millipore Sigma :
301 - 350 of 385 citations for rac Clopidogrel MP Endo Derivative 13C d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Control cultures were swapped from SM supplemented with 13C-glucose to SM solely supplemented with 12C-amino acids (12C-AA; Sigma #Y1896) at standard culturing concentrations.
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bioRxiv - Immunology 2020Quote: ... 5 × 106 activated B cells were washed with PBS and re-cultured in glucose-free medium RPMI medium containing 10% dialyzed FBS and uniformly labeled [13C]-glucose (2 g/L; Sigma-Aldrich) for 24 and 48 h ...
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bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
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bioRxiv - Molecular Biology 2023Quote: ... 15N labelled protein was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 15N isotopically labelled protein sample was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transferred to complete KSR media containing a final concentration of 4500 mg/L [U-13C]glucose 99% (CAT# CLM-1396-1, Sigma-Aldrich). This is the same concentration of unlabelled glucose normally found in the complete KSR media ...
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bioRxiv - Plant Biology 2024Quote: ... The dimethylation reaction was carried out by adding 2 µL of a 4% formaldehyde solution (Formaldehyde 13C, d2 solution, 596388, Sigma-Aldrich or Formaldehyde-D2 ...
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bioRxiv - Biochemistry 2024Quote: ... was dissolved in 1 ml 0.2 M acetate buffer (pH 5.6) containing 40 mM CaCI2 and 30% 13C-glycerol (w/w) (Sigma-Aldrich, Cat#489476). Forty micrograms of 1,2-dioleoyl-PC were dissolved in 1.7 ml diethyl ether ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼50 mg of frozen liver tissue (n = 5 per dose) was supplemented with 6 nanomoles of 13C and 15N labeled amino acid internal standards (767964, Sigma-Aldrich) and lysed with a Mixer Mill 300 and a 3 mm stainless steel ball in a solution of methanol/chloroform (1:2 v/v) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Before analysis the sample extracts were diluted 1:100 in an aqueous mix of isotope-labeled amino acids (algal amino acid mixture-13C-15N; Sigma-Aldrich). Chromatographic separation was done on a Shimadzu Nexera X3 equipped with an Agilent 1290 infinity II inline filter (0.3 µm ...
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bioRxiv - Genetics 2024Quote: ... coli OP50 grown in a M9 minimal medium supplemented with 13C-D-glucose (Cortecnec, France) and 1 g/L of 14N-ammonium chloride (Sigma-Aldrich) as sole carbon and nitrogen sources ...
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bioRxiv - Neuroscience 2021Quote: ... the four-month-old APP/PS1 mice under vitamin D3-sufficient diet condition were intraperitoneally injected weekly with 3 mg/kg of p53 inhibitor Pifithrin-α (PFTα, Sigma-Aldrich, Cat# P4359) for 3 months before harvesting hippocampal tissues for analysis ...
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bioRxiv - Neuroscience 2021Quote: ... We used synthetic AstA (Neo-MPS) and dopamine (Sigma-Aldrich). Crustacean cardioactive peptide (CCAP) ...
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bioRxiv - Biophysics 2022Quote: HK2p was dissolved in N-Methyl-2-pyrrolidone (MP) (Sigma) for channel experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Glycine (MP Biomedical-194825),Triton X-100 (Sigma-Aldrich-T8787), Albumin Bovine Fraction V (MP Biomedical-160069) ...
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bioRxiv - Cell Biology 2021Quote: ... 1992) and derivative mESCs clones were grown in serum/leukemia inhibitory factor (LIF)-containing stem cell medium: DMEM (EmbryoMax, Millipore, Burlington, MA), P/S (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... labeled with self-healing Cy3 and Cy5 derivatives (LD555-CD and LD655-CoA, respectively) and purified through an Optiprep™ (Sigma Aldrich) gradient as previously described 24 ...
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bioRxiv - Molecular Biology 2024Quote: Human A2780 cells (ovarian, adenocarcinoma) and the cisplatin-resistant derivative A2780cis subclone were purchased from Sigma-Aldrich (Cat#93112519 and Cat#93112517) and cultured in RPMI-1640 Medium ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Uric acid-13C 18O was synthesized from urea-13C 18O (Cambridge Isotopes, Andover, MA; Cat #COLM-4861) and 5,6-diaminouracil sulfate (Sigma-Aldrich; Cat #D15103) according to methods of Cavalieri et al (Cavalieri et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... The precultures were washed three times with DBM medium without carbon source and inoculated in the same medium but replacing the non-labeled carbon source with 13C-sodium formate (Sigma-Aldrich Co.), 1-13C-sodium acetate (Cambridge Isotope Laboratories Inc. ...
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bioRxiv - Immunology 2022Quote: ... For some experiments D-mice were fed corn oil alone or corn oil with one of two doses of vitamin D3 (Sigma-Aldrich, C9756, St. Louis, MO). Age and sex-matched Golden Syrian Hamsters were purchased from Envigo (Indianapolis ...
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bioRxiv - Cell Biology 2020Quote: ... FA-DNPH derivatives were detected with an ultraviolet detector (Cas: 228-34016, Shimadzu, Japan) and a C18 reversed-phase column (Sigma-Aldrich, #50208-U), using 65 % acetonitrile as the mobile phase.
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bioRxiv - Immunology 2021Quote: ... 6a-methylprednisolone (MP, Sigma Aldrich M0639, 20mg/kg every other day), and mycophenolate mofetil (MM ...
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bioRxiv - Systems Biology 2021Quote: ... 3 replicates of each cell line were grown for 48h in either growth medium with either [U-13C] glucose (Sigma-Aldrich, cat.no. 389374), [U-13C] glutamine (Cambridge Isotope Laboratories ...
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bioRxiv - Systems Biology 2020Quote: ... They were then filtered through 0.22-μm PTFE syringe filters and aliquots of 450 μl were diluted with 25 μl of acetonitrile and 25 μl of a 13C-caffeine (internal) standard solution (Sigma-Aldrich, Steinheim, Germany). These were injected in a liquid chromatography coupled with a quadrupole-time-of-flight mass analyser (LC-QTOF-MS ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Molecular Biology 2020Quote: ChIPs were performed on plasmid shuffled derivatives of KY3232 as described above with an antibody against the FLAG epitope (Sigma-Aldrich, 30 µL A2220) to immunoprecipitate FLAG-Rpb3 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 2 mM (final concentration) of either 12C-(natural isotope abundance control) or 13C-labeled sodium bicarbonate (Sigma-Aldrich, St. Louis, MS, USA). In addition ...
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bioRxiv - Systems Biology 2021Quote: ... including calcipotriol hydrate were purchased from Sigma-Aldrich (St. Louis, MPS, US) unless stated otherwise ...
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bioRxiv - Bioengineering 2021Quote: Cells and MPs were stained with PKH26 red fluorescent stain (Sigma-Aldrich) following the manufacture protocol to stain proteins in cells and MPs ...
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bioRxiv - Microbiology 2021Quote: ... Nor1 and Nurr1 agonists 6-mercaptopurine (6-MP) (Millipore-Sigma, Cat#38171) and amodiaquine (AQ ...
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bioRxiv - Microbiology 2021Quote: ... Nor1 and Nurr1 agonists 6-mercaptopurine (6-MP) (Millipore-Sigma, Cat#38171) and amodiaquine (AQ ...
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bioRxiv - Cancer Biology 2024Quote: ... it was incubated with 20mM Mercapto-propanesulfonate (MPS, Cat #251682, Sigma-Aldrich) in 10mM H2SO4 for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... L-[U-13C]proline (L-proline-13C5, 99 atom %), or L-[U-13C]leucine (L-leucine-13C6, 98 atom %) as indicated in Supplementary Table 1 (Sigma-Aldrich, St. Louis, MO, USA). Studies evaluating flow of amino nitrogen from Stickland-fermented leucine to [U-13C]pyruvate generated in glycolysis used L-[15N]leucine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Studies evaluating flow of amino nitrogen from Stickland-fermented leucine to [U-13C]pyruvate generated in glycolysis used L-[15N]leucine (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2021Quote: ... Stock solutions of Stable isotope-labeled mix (Algal amino acid mixture-13C,15N) for metabolomics approach were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
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bioRxiv - Cancer Biology 2024Quote: ... it was incubated with 20 mM Mercapto-propanesulfonate (MPS, Cat #251682, Sigma-Aldrich) in 10 mM H2SO4 for 30 minutes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Triton X-100 (MP biomedical: SKU:02194854-CF) and paraformaldehyde (Sigma-Aldrich: P6148-500G), 0.22 □m syringe filter (MERK ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescently-labelled MPs were manufactured by addition of 0.001% Coumatin-6 (Sigma-Aldrich, UK) as described previously (Qutachi et al. ...
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bioRxiv - Genetics 2024Quote: Cells were sequentially labeled with 30µM CldU (MP Biochemicals) and 250µM IdU (Sigma-Aldrich) according to the schematic in each figure ...
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bioRxiv - Bioengineering 2024Quote: ... the MPs were degraded over two hours with 200 µl dimethyl sulfoxide (DMSO) (Sigma Aldrich) and 600 µl of 0.2 M NaOH (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... CHO MPs were washed in PBS and resuspended in RIPA buffer (Millipore Sigma, Burlington, MA, USA) supplemented with protease inhibitor (200:1 buffer-to-inhibitor ratio) ...
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bioRxiv - Microbiology 2023Quote: ... MP-AU and enhanced AU containing final concentration 1 mM iron (II) sulfate heptahydrate (Sigma-Aldrich) was performed by Dr Lorna Eades at the University of Edinburgh using the 8900 Triple Quadrupole ICP-MS instrument (Agilent) ...
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bioRxiv - Immunology 2024Quote: ... After adsorption, 0.6% immunodiffusion-grade agarose (MP Biomedical, #952014) in DMEM (Millipore Sigma, #SLM-202-B) supplemented with 5% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... anesthesia was switched to α-chloralose (50 mg/kg/h IV, C0128, Sigma or 100459, MP Biochemicals) prior to data acquisition ...
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bioRxiv - Biochemistry 2022Quote: ... and 5% Goat Serum (Normal Goat Serum [Millipore], 0.5% Triton-X-100 [Sigma-Aldrich], 0.05% Sodium azide [MP]). Measurement of protein levels transpired with 1x phosphate buffered saline ...
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bioRxiv - Synthetic Biology 2020Quote: ... adjusted to pH = 8 with 2 M NaOH and syringe-filtered (Millex® MP 0.22 μm, Millipore Sigma). Extreme caution was taken to prohibit contamination ...
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bioRxiv - Immunology 2021Quote: All MP measurements were performed in Tris buffer (25 mM Tris, 100 mM NaCl, pH 7.6 (Sigma Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... MPs were then incubated overnight with the primary anti-TOM20 rabbit polyclonal antibody (Sigma Life Sciences, cat. # HPA011562) diluted 1:100 in 0.2% BSA-containing PBS ...