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Citations for Millipore Sigma :
301 - 350 of 5716 citations for Recombinant Human Granzyme B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Genomics 2023Quote: The human pre-B ALL cell line Reh (DSMZ, ACC 22) was maintained in RPMI-1640 medium (Sigma, R0883) supplemented with 2 mM L-Glutamine (Sigma ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Cytochalasin B (Cyt B; produced by Drechslera dematioidea, Sigma-Aldrich). To ingest particles ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Cell Biology 2023Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cytochalasin B (Sigma), and 1,2-dipalmitoyl-sn-glycerol (Sigma D9135)
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bioRxiv - Microbiology 2020Quote: ... Polymyxin B (Sigma) was used as a positive control due to its outer membrane permeabilizing property.
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bioRxiv - Cell Biology 2022Quote: ... Latrunculin B (Sigma) was dissolved freshly in MFSW from frozen aliquots at 20 mM in DMSO ...
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bioRxiv - Biophysics 2020Quote: ... Latrunculin B (Sigma) was resuspended in DMSO to a concentration of 12.6 mM and used at 1μM for 1 hour before AFM measurements.
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bioRxiv - Cancer Biology 2021Quote: ... B-actin (Sigma), Total PARP (CST) ...
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bioRxiv - Microbiology 2023Quote: ... CelLytic B (Sigma) was included as positive control.
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bioRxiv - Physiology 2024Quote: ... cocktail B (Sigma) and for Na+ measurements we used a Na+ ionophore X (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...