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Citations for Millipore Sigma :
301 - 350 of 7937 citations for Recombinant Canine EGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: GST and Recombinant Virp1-GST proteins were expressed in Escherichia coli Rosetta strain (EMD Millipore, Burlington, MA). Cells were grown overnight at 37°C in LB media supplied with ampicillin (0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The expression of the recombinant proteins was checked by Western blotting with monoclonal anti-HA antibodies (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... Cellular RAS activity was stimulated with 50ng/ml EGF (Sigma). Were indicated ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml EGF (Sigma, St. Louis, Missouri, USA, E9644), 500 μg/ml hydrocortisone (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... antibiotics (as above) and 5 ng/mL EGF (Sigma, E9644). All cell lines were maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml EGF and 20 ng/ml bFGF (Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... and 5 ng/ml epidermal growth factor (EGF; Sigma-Aldrich). Total RNA was then extracted using RNeasy Mini Kit (Quiagen ...
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bioRxiv - Cell Biology 2022Quote: ... epidermal growth factor (EGF) (20 ng/ml) (Sigma-Aldrich; E9644), cholera toxin (100 ng/ml ...
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bioRxiv - Microbiology 2019Quote: ... and 20 ng/ml epidermal growth factor (EGF) (Sigma-Aldrich). Upon 65% confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... and epidermal growth factor (EGF) (#E9644) were from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2021Quote: ... 1% glutamine and supplemented with EGF (.01 ug/ml, Sigma), insulin (5 ug/ml) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% glutamine and supplemented with EGF (0.01 ug/ml, Sigma) and insulin (5 ug/ml ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 20ng/ml FGF (R&D) and EGF (Sigma), 1% N-2 Supplement (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 5μg/mL hydrocortisone and 20 ng/ml EGF (Sigma-Aldrich) to induce the expression of CSN-2 ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 50 ng/mL EGF (Sigma-Aldrich, E9644-.2MG), 100 ng/mL Noggin (R&D ...
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bioRxiv - Developmental Biology 2024Quote: ... 50 ng/mL epidermal growth factor (EGF, E9644, Sigma-Aldrich), 2 μM CHIR99021 (04-0004 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 ng ml-1 epidermal growth factor (EGF; Sigma–Aldrich), 1% Penicillin-Streptomycin solution (Sigma– Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... EGF-stimulated A431 cell lysate control (Sigma Aldrich #12-302), and E pacifica control lysate (lysed as for S ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 ng/ml epidermal growth factor (EGF; E4127, Sigma). Cells were used up to passage 5 ...
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bioRxiv - Microbiology 2019Quote: ... with eight bi-directional PHW2000 plasmids containing the appropriate influenza A virus segments and coculture in Madin-Darby Canine Kidney (MDCK) cells (ATCC) with addition of 2μg ml−1 of TPCK treated Trypsin (Sigma-Aldrich). Rescued viruses were passaged once in embryonated hen’s eggs to generate working stocks ...
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bioRxiv - Microbiology 2021Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney 293T (293T) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Influenza viruses as well as vesicular stomatitis virus strain Indiana (VSV) were propagated on Madin-Darby canine kidney (MDCKII) cells cultured in minimal essential medium (MEM-Sigma) containing 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: MDCK (Madin-Darby canine kidney, obtained from ATCC) were grown at 37°C in 5% CO2 in MEM medium (Sigma) containing 5% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: Madin–Darby canine kidney (MDCK) cells were cultured in minimum essential medium (MEM; Sigma-Aldrich Co. LLC, St. Louis, MO) supplemented with 5% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... was grown in Madin-Darby canine kidney (MDCK) cells with serum-free MEM (HyClone, Cytiva) containing 1 IU/mL of trypsin (Sigma), 1 μg/mL EDTA (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Recombinant protein was then isolated from the supernatant by nickel affinity chromatography using loose Ni-NTA resin (Sigma). Protein eluted from Ni-NTA resin was filtered and then loaded onto a size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Cell Biology 2021Quote: ... FAK) recombinant proteins to be labeled with Alexa fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentration of total protein in CFE with recombinant Scaf19LKT was determined by Bradford reagent (Sigma-Aldrich, USA), CFE was diluted 1,000-times in 0.1 M sodium carbonate coating buffer (pH 9.0) ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2.5μg/ml of recombinant RBD protein in carbonate-bicarbonate coating buffer (Sigma). After blocking with PBS containing 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... except for the control group for which recombinant fusion proteins were mixed with incomplete Freund adjuvant (IFA, Sigma). Blood samples were collected from the submandibular vein at different time points for more than 6 months and sera were prepared and analysed for the presence of specific antibodies by ELISA as described below ...
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bioRxiv - Immunology 2022Quote: ... a control group received injection of recombinant fusion proteins (3.8 μM) mixed with complete Freund adjuvant (CFA, Sigma) in 50 μl of PBS ...
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Microbiology 2023Quote: Madin-Darby canine kidney (MDCK) cells were grown in Roswell Park Memorial Institute (RPMI)-1640 medium (Sigma Aldrich, Castle Hill, Australia) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cell Biology 2020Quote: ... UK),10 ng/ml epidermal growth factor (EGF) (Sigma-Aldrich, Gillingham, UK), 1 mg/ml human insulin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml epidermal growth factor (EGF) (Sigma-Aldrich, Gillingham, UK) and 5 ug/ml human insulin (Sigma-Aldrich ...