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Citations for Millipore Sigma :
301 - 350 of 9992 citations for LIF Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ESC were withdrawn of 2i/LIF for 48 hours and plated at single cell density (50 cells/cm2) in N2B27 media with 2i/LIF on plates coated with 0.1% gelatin (Sigma). At day 5 ...
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bioRxiv - Developmental Biology 2022Quote: ... ~1.5×107 cells were plated in 10-cm dishes in media without LIF and supplemented with 10μM all-trans retinoic acid (Sigma). Cells were kept with constant rotation for 4 days and media was changed every other day ...
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bioRxiv - Developmental Biology 2022Quote: ... serum-LIF-grown ESCs were split with accutase onto laminin-coated 6-well/plates (Sigma, 10μg/ml in PBS) at a density of 0.75×104 cells/well in plain N2B27 medium ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 ng/ml Leukaemia Inhibitory Factor (LIF, produced in-house following a protocol by Tomala et al., 2010) and 0.1 mM beta-mercaptoethanol (Sigma). Chicken DF-1 (Doug Foster strain 1 ...
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bioRxiv - Genomics 2021Quote: ... TX1072 derived mutant cell lines and 1.8 cells were grown on 0.1% gelatin-coated flasks in serum-containing medium supplemented with 2i and LIF (2iL) (DMEM (Sigma), 15% ESC-grade FBS (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... or and 3) without 2i and LIF supplemented with 0.25 M all-trans-retinoic acid (Sigma-Aldrich, Cat #R2625), and cultured for 4 days.
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bioRxiv - Genetics 2024Quote: mESCs were grown on 0.1% gelatin-coated flasks in serum-containing medium supplemented with 2i and LIF (2iL) (DMEM (Sigma), 15% ESC-grade FBS (Gibco) ...
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bioRxiv - Developmental Biology 2019Quote: ... were cultured on gelatin-coated tissue culture plates in the presence of sterile-filtered ESC media with leukemia inhibitory factor (LIF) (ESGro Millipore), ES-qualified FBS (Gemini Bioproducts) ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were thawed on 0.2% gelatin in serum/LIF and after 24h seeded at a density of 0.6 x105 cells/cm2 in 2i/LIF medium on a dish coated with 0.01% poly-L-ornithine (Sigma-Aldrich, P3655) and 500 ng/ml laminin (Corning ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 μg/mL penicillin/streptomycin and 1×106 units/mL leukemia inhibitory factor (LIF; ESGRO, EMD Millipore, catalog no. ESG1106) in a humidified 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... the ligands and small molecule inhibitors used in this study and their concentration were: LIF (1,000 units/ml, Millipore ESG1106), BMP4 (10 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with Small molecule inhibitors PD0325901 (PD, 1 μM, Selleck) and CHIRON99021 (CH, 3 μM, Selleck) and 1000 U/ml LIF (ESGRO, Millipore). Cells were routinely propagated on 0.1% gelatin-coated plastic and replated every 3 days at a split ratio of 1 in 10 following dissociation with Accutase (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Around 1000 mESCs were suspended in 20 µl of differentiation medium (mESCs media without LIF, PD0325901 and CHIR99021, supplemented with 1µM retinoic acid (Sigma-Aldrich). After 3 days ...
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bioRxiv - Molecular Biology 2022Quote: Murine embryonic stem cells were cultured in 2i/LIF media on tissue culture plates coated with 0.2% gelatin (Sigma, G1890). The 2i/LIF media contained ...
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bioRxiv - Developmental Biology 2023Quote: ESCs were differentiated to EPL cells by culturing 20,000 cells/cm2 in EPL cell medium (90% DMEM, 10% FBS, Pen/Strep, 0.1 mM β-Me, 330 U/mL LIF, 400 μM L-proline, Sigma) for 6 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin, Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin/Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/mL ...
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bioRxiv - Genomics 2021Quote: mESCs were grown at 37 °C under a 95% air and 5% CO2 atmosphere on gelatin in serum/LIF medium composed of GMEM (Sigma, G5154) supplemented with 15% FBS (HyClone™) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1987) and were routinely cultured on 0.1% gelatin coated plates in serum/LIF medium [Glasgow Minimum Essential Medium (Sigma-Aldrich; G5154), 10 % foetal calf serum ...
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bioRxiv - Cell Biology 2024Quote: ... mESCs were split and approximately 6 × 104 cells were plated onto a 12-well plate coated with 1% matrigel in ESCs medium without LIF and the next day medium was replenished with differentiation medium containing 40% MCDB (Sigma-#M6770), 100µM β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2023Quote: ... 3.0 × 103 iMeLCs were plated into ulta-low attachment U-bottom 96-well plate (PHCBI, MS-9096UZ) in GK15 supplemented with: 10 ng/ml LIF (Millipore, LIF1005), 200 ng/ml of BMP4 (314-BP-010 ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 500nM 4-hydroxytamoxifen (4-OHT) for 72h in 5i+LIF (Supplementary Table1) with 100 μM apoptosis inhibitor Z-vad (Sigma-Aldrich®) and 50 μM of necroptosis inhibitor Necrostatin-1 (Sigma-Aldrich®) ...
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bioRxiv - Cell Biology 2020Quote: ... This basal medium was supplemented with 2i-LIF (3 µM CHIR99021, Sigma-Aldrich, cat. no. SML1046 and 1 µM PD0325901 Sigma-Aldrich, cat ...
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bioRxiv - Cell Biology 2021Quote: ... 1992) and derivative mESCs clones were grown in serum/leukemia inhibitory factor (LIF)-containing stem cell medium: DMEM (EmbryoMax, Millipore, Burlington, MA), P/S (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was changed to 2i + LIF medium consisting of base medium supplemented with CHIR99021 (Chiron, 3 µM, Sigma-Aldrich SML1046-5MG), PD0325901 (1 μM ...
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bioRxiv - Molecular Biology 2019Quote: ... and the mono-parental ESC lines PR8 (39) and AK2 (40) were maintained without feeders on gelatin-coated dishes in serum-free ESGRO Complete PLUS medium (Millipore, with LIF and Gsk3 inhibitor). Differentiation of ESCs into cortical neural progenitor cells (NPCs ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mouse αSMA (Sigma A2547), goat-anti-human TIE1 (R&D AF619) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-mouse IgG (mouse, A9044, 1:10000,Sigma) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-STIM1 (Novus) or mouse anti-FLAG (Sigma) were used for IF staining ...
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bioRxiv - Cell Biology 2023Quote: ... Normal Mouse IgG (12-371, Sigma-aldrich, Mouse polyclonal).
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mg/mL mouse anti-Myc (EMD Millipore, 4A6 [mouse]), 1 mg/mL rabbit anti-Myc (EMD Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti mouse Nestin (Millipore, product no: MAB353, (1:100)) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-γ tubulin and mouse anti-a tubulin (Sigma). Goat anti-mouse IRDye 680RD and goat anti-rabbit IRDye 800CW (LI-COR Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... anti-mouse (Sigma), 1:10,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse IgG (Sigma), and rat IgG (Sigma).
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bioRxiv - Plant Biology 2020Quote: ... anti-mouse (Sigma) or anti-rabbit peroxidase-conjugated (Merck Millipore ...
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bioRxiv - Neuroscience 2019Quote: ... Flag (Mouse, Sigma), vGLUT1 (Guinea Pig ...
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bioRxiv - Microbiology 2019Quote: ... β-actin (mouse, Sigma), GAPDH (mouse ...
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bioRxiv - Neuroscience 2019Quote: ... Flag(mouse, Sigma), GST(rabbit ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP(mouse, Sigma), PSD-95(guinea pig ...
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Lineage, Identity, and Fate of Distinct Progenitor Populations in the Embryonic Olfactory EpitheliumbioRxiv - Developmental Biology 2021Quote: ... pSMAD (Millipore, mouse), BrdU (BD ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse IgG (Sigma), rat IgG (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... H3S10P (Mouse Millipore Cat#06570 multiple Lot# ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse mAb (Sigma) at 1:1000 (only used for Figure S1).