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Citations for Millipore Sigma :
301 - 350 of 705 citations for IFNAR1 Rhesus Macaque HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Cancer Biology 2020Quote: ... constructs were transfected with packaging vectors into HEK-293T with Fugene HD (Sigma-Aldrich, St. Louis, MO). After plating ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells were maintained in DMEM10 containing 100 µg/ml G418 (Sigma Aldrich. St Louis, MO). African green monkey kidney cell line Vero E6 ATCC #CRL-1586 were cultured at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s high glucose medium (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and HEK 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6429; Millipore Sigma, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: Human embryonic kidney HEK-293T were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma F8067) and gentamycin (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or HEK-ssCXCR2-tsNluc cells were washed twice with phosphate-buffered saline (PBS, Sigma-Aldrich, Pool, UK) to remove excess labelling or growth medium before being removed by scraping into 10ml PBS ...
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bioRxiv - Cell Biology 2023Quote: HEK cells were grown in an 8-well chamber slide (Millicell EZ slide, Merck-Millipore, Burlington, MA) to early confluence ...
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bioRxiv - Cancer Biology 2023Quote: ... constructs were transfected with packaging vectors into HEK-293T with Fugene HD (Sigma-Aldrich, St. Louis, MO). After plating ...
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bioRxiv - Biochemistry 2023Quote: HEK cells transfected with ERα or ERβ as appropriate were fixed with 4% paraformaldehyde (SIGMA, ref: 1635) in PBS for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... These were transfected into Flp-IN 293 Trex K(AAA)21 wt and ZNF598 ko using Genejuice (Novagen). 24 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: TGF-β1 or INT-767 treated HK2 or 293-T cells were harvested in RIPA buffer (Sigma # R0278); 200 µg cell protein in 400 μL volume was added to 2 µg primary antibody and gently rotated at 4°C overnight ...
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bioRxiv - Genomics 2024Quote: ... All Freestyle 293-F cells containing a landing pad were induced with 2 μg/mL doxycycline (Sigma-Aldrich).
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK 293T cells were carried out using the X-tremeGene 9 DNA Transfection Reagent (Sigma-Aldrich) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Microbiology 2022Quote: ... The Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Developmental Biology 2021Quote: ... HEK cell aggregates were placed in the middle of a drop of 20 μl chicken plasma (Sigma-Aldrich) on non-coated HNO3-cover slips ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were cultured in DMEM (high glucose, Sigma-Aldrich, SH30022.01) with 10% FCS (Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Microbiology 2023Quote: ... University of Lausanne) and Human Embryonic Kidney (HEK) 293T cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, cat. no. D6429) supplemented with 10% heat-inactivated foetal bovine serum (hiFBS ...
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bioRxiv - Immunology 2023Quote: ... HEK-293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium (Cat# D8437, Sigma, MO, USA) supplemented with 10% fetal bovine serum (FBS ...