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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Human E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
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bioRxiv - Genetics 2021Quote: ... Mouse plasma insulin and leptin levels were determined by using Rat/ Mouse Insulin ELISA Kit (Millipore, EZRMI-13K for insulin and EZML-82K for leptin ...
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bioRxiv - Microbiology 2021Quote: ... the antibodies isotypes were determined by ELISA using a mouse Ig isotyping kit (ISO-2, SIGMA).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 125 μl of lysis buffer provided with a CAT ELISA kit (Sigma). CAT expression and Renilla luciferase activities were measured and the ratio was calculated.
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Cell Biology 2020Quote: ... embryos were manually dechorionated in E3 medium using watchmaker forceps (Dumont No.55) and mounted in 0.7 % low melting-point agarose (Sigma-Aldrich) in glass capillaries (Brand Transferpettor caps ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... Embryos were mounted in 0.6% low melting point agarose (prepared in E3) supplemented with 240 μg/mL of MS-222 (Sigma-Aldrich) as previously described 52 ...
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bioRxiv - Developmental Biology 2019Quote: ... with 0.4% low melting agarose (MO BIO laboratories) in E3 embryo medium containing 180mg/ml tricaine (MS-222, Sigma-Aldrich). After the agarose solidified ...
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were manually dechorionated and mounted in glass capillaries in 0.9% low-melting-point agarose (in E3) supplemented with 240 μg/mL of tricane methanesulfonate (MS-222; Sigma-Aldrich). The sample chamber was filled with E3 medium containing 0.01% MS-222 and 0.2 mM PTU (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and 25 ng/μl E3 (HUWE1, catalytic domain, aa 3228-4374/1147 aa) were incubated with 0.3 μg/μl Ub (Sigma-Aldrich) and 11 ng/μl H1.2/H1.2 KxAc in 2 mM ATP ...
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bioRxiv - Molecular Biology 2020Quote: ... embryos were cultivated at 28.5 °C for 3 days in fresh standard E3 medium supplemented with 0.05% methylene blue (Sigma Aldrich, MO) and 0.003% phenylthiourea (PTU ...
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bioRxiv - Cancer Biology 2019Quote: ... larvae were anesthetized in E3 media without methylene blue containing 0.16 mg/ml Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich). Zebrafish lines used are summarized in Table 1.
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bioRxiv - Developmental Biology 2020Quote: 30 embryos were transferred at 52 hpf (3 hours post heat shock) into a 35 mm petri dish containing 5 mL E3 embryo media plus 12.5 mM MG132 (Sigma-Aldrich), or an equal volume of vehicle (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos older than 24 hpf were transferred into E3 medium containing 0.003% 1-phenyl 2-thiourea (PTU, Sigma-Aldrich P7629). Animal work was approved by the local Animal Welfare and Ethical Review Body (AWERB ...
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bioRxiv - Cancer Biology 2023Quote: ... larvae were anesthetized in E3 (embryo medium) without methylene blue containing 0.16 mg/ml tricane (MS22/ethyl3-aminobenzoate - Sigma Aldrich). Transgenic line expressing BFP under the lyzC promoter in the membrane of neutrophils Tg(lyzC:BFP)zf217Tg and transgenic line expressing eGFP under the fli1 promoter of endothelial cells Tg(fli1:EGFP)y1Tg were used in this study (4) ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were grown at 28°C in E3 medium containing 1-phenyl-2-thiourea (0.003%, PTU, Sigma-Aldrich, P7629-10,) to avoid pigmentation ...
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bioRxiv - Cell Biology 2023Quote: ... 3 days post-fertilization (dpf) larvae were anesthetized in E3 medium containing 0.2 mg/mL Tricaine (ethyl 3-aminobenzoate; Sigma-Aldrich) and maintained at 28.5 °C ...
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bioRxiv - Cell Biology 2023Quote: ... was added to the E3 medium of the control group.30 The final concentrations of 80 µM MTZ (Sigma Aldrich) and 50 nM NFP (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used at a 1:500 concentration: anti-K48 ubiquitin (AB_11213655, Millipore), anti-HA (AB_390919 ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μM Cy5-ubiquitin (South Bay Bio) and 10 mg/mL bovine liver glycogen (Sigma) in Phosphate Buffered Saline pH 7.4 containing 0.5 mM TCEP and 5 mM Mg2+-ATP ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then incubated with a rabbit anti-phospho-ubiquitin (Ser65) antibody (Merck Millipore, Burlington ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Immunology 2020Quote: ELISA plates (Cat. M5785, Sigma Aldrich) were first coated overnight at 4°C with 10μg/ml of each B cell peptide epitope ...
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bioRxiv - Molecular Biology 2023Quote: ... Ghrelin ELISA (EZRGRA-90K)(Sigma Aldrich) IGF-1 ELISA (Catalog #80574 ...
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bioRxiv - Immunology 2022Quote: – Antibody enzyme conjugates for ELISA (Sigma): Peroxidase conjugated Anti-mouse polyvalent immunoglobulins (IgG ...
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bioRxiv - Cell Biology 2020Quote: ... Embryos were mechanically dechorionated and mounted in 1% low melting-point agarose in E3 medium supplemented with 20% w/v Iodixanol (Sigma-Aldrich) to minimize refractive index mismatch (60 ...
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bioRxiv - Immunology 2021Quote: ... larvae were anesthetized in E3 media without methylene blue supplemented with 0.16 mg/ml Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... drug stocks were diluted in 28°C E3-MS-222 medium (istaroxime: MedCham Exptress, Lot#11394, ouabain: Sigma-aldrich, Lot#BCBZ9329) and the medium was mixed vigorously to assure a homogeneous concentration of the drug ...
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bioRxiv - Biochemistry 2022Quote: The following reagents were used in this study: the Von Hippel-Lindau (VHL) E3 ligand (S,R,S)-AHPC-PEG3-azide (AHPC) (Sigma Aldrich), MG132 (Sigma Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... The severe hepatocyte injury model included incubating the zebrafish larvae in E3 medium supplemented with 10 mM acetaminophen (Sigma-Aldrich, A7085), and 0.5% Ethanol for 48 hours from 3 to 5 dpf ...
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bioRxiv - Microbiology 2022Quote: Seventy-two hour post-fertilised larvae were first anaesthetized for 2 - 3 min in embryo medium E3 containing 0.2 mg/mL Tricaine (Sigma-Aldrich, USA). After which ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos and larvae were raised until 5 days post fertilization (dpf) in E3 medium containing methylene blue or in 1-phenyl-2-thiourea (PTU, Sigma-Aldrich) if prevention of pigment formation was required ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...