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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... BSA (0.5% wt/vol, Sigma-Aldrich), SUPERase in (0.2 U/μL ...
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bioRxiv - Neuroscience 2021Quote: ... 50 wt% final concentration sucrose (Sigma) and 10 wt% final concentration triethanolamine (Aldrich Chemistry ...
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bioRxiv - Microbiology 2021Quote: ... or 0.02% (wt/vol) cholesterol (Sigma). These cell suspensions were then mixed with 100 µl of freshly prepared 2x bismuth solution in clear flat-bottomed 96-well microtiter plates (Costar) ...
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bioRxiv - Bioengineering 2020Quote: ... 10% (wt/vol) Acrylamide (Sigma, A3553), 0.1% (wt/vol ...
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bioRxiv - Microbiology 2023Quote: ... 0.21% (wt/vol) sodium bicarbonate (Sigma), and 10% (vol/vol ...
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bioRxiv - Biophysics 2023Quote: ... 0.0425% (wt/vol) Pluronic F108 (Sigma) supplemented with 1 mM IPTG.
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bioRxiv - Bioengineering 2024Quote: ... 0.5 wt.% BSA (A4612, Sigma-Aldrich), and 0.5 wt.% methylcellulose (M7027 ...
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bioRxiv - Molecular Biology 2020Quote: ... potassium cyanide (KCN; #60178) for the MRC complex IV and antimycin A (AA; #A8674) for the MRC complex III (purchased from Sigma-Aldrich).
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bioRxiv - Bioengineering 2020Quote: A 4 wt.% poly(ethylene oxide) (PEO, Mv = 900,000 g mol−1, Sigma-Aldrich) solution was prepared by dissolving PEO in DI water and stirring the solution overnight on a hotplate set to 48 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Crosslinking solution 1: PBS with 4% (wt/vol) acrylamide (Sigma-Aldrich, St. Louis, MO) and 0.25% (wt/vol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were fixed and stained with 1% (wt/vol) Rhodamine B (Millipore Sigma, R6626). Colony diameter was measured from scanned images of plates using ImageJ (v2.14.0/1.54f ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Plant Biology 2024Quote: ... and mouse anti-His (Novagen).
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Biochemistry 2024Quote: Human C-reactive protein (Cat. No. C4063) and Human erythrocyte catalase (Cat. No. C3556) were obtained from Sigma Aldrich chemicals ...
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bioRxiv - Immunology 2021Quote: ... 1 U/kg human insulin (Sigma) was given i.p ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/mL human fibrinogen (Millipore), and 10 U/mL heparin (Sigma-Aldrich)).
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bioRxiv - Cell Biology 2023Quote: ... 1 ug/ml human insulin (Sigma), 10 nM hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:2000 human anti-CREST (Sigma) and donkey anti-human Dylight550 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1 % human albumin (Sigma). PBMC were thawed and rested overnight in X-Vivo-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines stably expressing the WT/mutant proteins were selected using media supplemented with 5 μg/ml puromycin (Sigma-Aldrich). The reconstitution of mutant GSECs was assessed by SDS-PAGE/western blotting ...
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bioRxiv - Plant Biology 2023Quote: ... The fusion protein was cleaved using Thrombin (from bovine plasma, 50 NIH units/mg dry wt) following the manufacturer’s instructions (Sigma-Aldrich). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were expressed in bacteria and purified via the 6xHis tag using Ni-NTA His•Bind Resin (Novagen). The purified protein segments were then incubated with a pre-designed peptide array (Intavis) ...
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bioRxiv - Biochemistry 2024Quote: ... SUMO2-fusion proteins with Hexa-histidine (His6) tags were purified from HIS-Select Nickel (Ni2+) Affinity Gel resin (Sigma) through Poly-Prep Chromatography hand-columns (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Either 350 µl of purified CD95L-IZ-His (0.409 mg/ml) or a standard protein mix at 5.5 mg/ml (Sigma-Aldrich) was loaded and eluted at 0.3 ml/min flow rate with 0.3 ml fraction size in PBS buffer ...
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bioRxiv - Biophysics 2021Quote: ... A 50 µl drop of monomer solution (2 M NaCl, 2.5% (wt/wt) acrylamide (AA, A9099, Sigma), 0.15% (wt/wt ...
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bioRxiv - Neuroscience 2022Quote: ... Complex lipid removal was achieved incubating the samples in a 2:1 mixture of dichloromethane (Sigma-Aldrich) and methanol overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was purified using its incubation with 1 ml of anti-FLAG M2 affinity gel (Sigma) 6h at 4CC ...
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... were isolated from either naïve or infected or tumour-bearing HHD or BALB/c mice and were cultured in the presence of HLA-A2 restricted IAV M1 peptide 158-66(GILGFVFTL)(Cambridge peptide) or NY-ESO-1 peptide 157-65 (SLLMWITQC)(Sigma peptide) or H-2Kd binding IAV NP peptide 147-55(TYQRTRALV ...
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bioRxiv - Immunology 2022Quote: ... 2 mM vanadyl-ribonucleoside complex (Sigma, St. Louis, MO), 0.02% RNAse-free BSA (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... in complex with fatty-acid free BSA (Sigma-Aldrich) at a molar ratio of 6:1 added 4 hours after seeding ...
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bioRxiv - Biochemistry 2023Quote: Short oligos for EMSA and complex formation (Sigma-Aldrich) were reconstituted with TE buffer (10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... and Peroxidase anti-Peroxidase Soluble Complex antibody (P1291, Sigma) were also used.
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bioRxiv - Molecular Biology 2023Quote: ... anti-complex I – 75 kDa subunit (ABN302, EMD Millipore), anti-complex III ubiquinol-cytochrome C reductase core protein 2 (UQCRC2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2-hydroxypropyl-β-cyclodextrin complex (HBC; H107; Sigma-Aldrich) was used as vehicle control ...
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bioRxiv - Cell Biology 2024Quote: ... 10mM Complex V buffer (0,1M Tris (Sigma-Aldrich #T1503), 0,01M MgCl2 (Sigma-Aldrich #M9272) ...
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bioRxiv - Cancer Biology 2024Quote: ... 4.48 mg of 2-picoline borane complex (Sigma-Aldrich) in 25 µL of the same solvent was added and incubated for another 1.5 hour ...
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bioRxiv - Genomics 2020Quote: Gel-shift assays were performed using recombinant Human IRF2 protein (Sigma, #SRP6338). 10 pmoles of single-stranded oligos for surface fish IRF2 binding site and corresponding mutant cavefish IRF2 site were end-labeled with P32 radioisotope using polynucleotide kinase ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Developmental Biology 2024Quote: The rabbit anti-RFX antibody from Sigma was raised against 270-405 aa of human RFX3 protein (HPA035689, Sigma) and another anti-RFX3 antibody against 1-413 aa of human RFX3 were both used (14784-1-AP ...
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bioRxiv - Cell Biology 2021Quote: ... Replenishment of cholesterol was performed by incubating the cholesterol-depleted cells in 10 mM MβCD-cholesterol complex (1:1 mol/mol; Sigma Aldrich: C3045) at 37°C for 30 min38 ...
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bioRxiv - Immunology 2020Quote: ... HLA-peptide complexes were loaded on preconditioned 50mg C18 desalting columns (Sigma Millipore), then followed three 0.1% TFA in water washes ...