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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Preparation of nuclear and cytoplasmic fractions was performed according to the user manual supplied with the CelLytic™ PN Plant Nuclei Isolation/Extraction Kit (Sigma) with minor modifications ...
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bioRxiv - Immunology 2021Quote: ... Bone marrow isolations were done by collecting left and right tibia and flushing bones with 5ml Hanks Balanced Salt solution (HBSS) (Sigma-Aldrich) using a syringe with a 26G needle ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm lengths of leaf tip) using the ‘Crude Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich). DNA extraction from crude nuclei was performed using two different methods ...
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bioRxiv - Neuroscience 2023Quote: ... we prepared sucrose gradients with Nuclei PURE Sucrose Buffer and Nuclei PURE 2M Sucrose Cushion Solution from the Nuclei PURE Nuclei Isolation Kit (NUC-201, Sigma-Aldrich), and samples were carefully overlaid and centrifuged at 13,000 g for 45 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacture’s protocol with the following modifications: a) RNA isolation was performed in the dark and b) DTT (Sigma Aldrich #43816) was added to the aqueous phase (final concentration 0.1 mM ...
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bioRxiv - Biophysics 2023Quote: ... cardiomyocytes were prepared by cutting frozen tissue over dry ice in 5-10 mg pieces and immediately incubating in ice-cold (0°C) isolation buffer with 0.3% Triton X-100 in the presence of protease (Sigma-Aldrich, MO) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Biophysics 2022Quote: ... Total genomic DNA from cecal digesta was extracted using PowerSoil® DNA Isolation Kit (Sigma-Aldrich, Carlsbad, United States of America). DNA concentration and purity were assessed using a Synergy HTX (Gene Company Limited ...
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bioRxiv - Genomics 2023Quote: ... IGEPAL CA-630 was used for the lysis buffer a 10% solution of which was prepared fresh on the day of nuclear isolation using 100% stock (Sigma i8896). After trypsinization and pelleting ...
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bioRxiv - Cell Biology 2023Quote: ... treated with LNPs containing mRNA sequences of either the anti-NSP9 nanobody or tdTomato were used for total mRNA isolation by TRI Reagent (Millipore-Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Then after having carried out a pre-isolation on a 40%-23%-20%-11% ficoll gradient (Sigma Ficoll type 400, F4375), the islets were hand picked and maintained overnight à 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Debris was removed with density gradient centrifugation using Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer from the Nuclei PURE prep isolation kit (no. NUC201-1KT, Sigma Aldrich). Nuclei PURE 2 M sucrose cushion solution and Nuclei PURE sucrose cushion buffer were first mixed in a 9:1 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... the tissue was homogenized with two strokes at 300 rpm using a loose-fitting Teflon homogenizer followed by nitrogen cavitation at 800 psi for 10 min in ice cold isolation buffer supplemented with 0.5% fatty acid free BSA (Sigma-Aldrich, A7030) and protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... The sample was then spun at 1,000g for 3 min and resuspended in 0.5 mL Nuclei Isolation Reagent (NIR, S2 Genomics, #100-063-396) with 0.1 ul/uL RNase inhibitor (Protector, Millipore Sigma, #3335399001); all subsequent solutions had RNase inhibitor ...
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bioRxiv - Genetics 2021Quote: Exponentially growing cells at OD600 nm = 0.2 were injected into the microfluidics chambers of a CellASIC plate (Y04C-02 plate for haploid cells, CellASIC ONIX2 Microfluidic System, Millipore), through the inlet wells ...
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bioRxiv - Genomics 2020Quote: ... Cells were imaged in standard imaging buffer with an oxygen scavenging system containing 0.5 mg/mL glucose oxidase (Sigma-Aldrich), 40 μg/mL catalase (Roche or Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... These plasmids were transfected into Rossetta 2 cells and this system was used for expression according to manufacturer’s instructions (EMD Millipore) for expression ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... KSRP post-translational modifications were monitored in Huh7.5-SGR and Huh7.5 cells grown on Nunc Lab-Tek Chamber Slide™ system 8 wells (Permanox, Sigma-Aldrich), 10,000 cells/well ...
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bioRxiv - Cell Biology 2020Quote: ... mid-log phase fission yeast cells in YEA were placed into a Y04C-02 microfluidic chamber controlled by a CellASIC Onix2 system (CAX2-S0000; Millipore). 300 μl YEA was placed into the flow-wells ...
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bioRxiv - Neuroscience 2021Quote: ... or by either bath application or a custom-made perfusion system (for imaging cells cultured on 12-mm coverslips): ATP (Sigma), ADP (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and then samples of 40 μg were separated on SDS–PAGE gel in Bio-Rad Mini-PROTEAN Vertical Electrophoresis Cell System and blotted to the Immobilin-P membrane (IPVH00010, Millipore) in transfer buffer (25 mM Tris–HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... HRP/DAB system (Millipore Sigma) was used for detection ...
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bioRxiv - Cancer Biology 2020Quote: The Duolink Detection System (Sigma) was used to assess c-Met/β1 complex formation as previously described (7).
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bioRxiv - Neuroscience 2021Quote: ... 2.0 Protein Detection System (Millipore). Membranes were imaged using a chemiluminescent SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2022Quote: ... quick immunoblot vacuum system (Millipore) was used for washing steps and secondary antibody incubations ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... system (Millipore, Billerica, MA, USA), followed by incubation with secondary antibodies (IRDye 800CW 1:1000 in PBS/Tween ...
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bioRxiv - Neuroscience 2022Quote: ... followed by chemiluminescence system (Millipore).
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bioRxiv - Neuroscience 2022Quote: ... 2.0 Protein Detection System (Millipore). The following antibodies were used ...
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bioRxiv - Physiology 2022Quote: ... system (Millipore, Billerica, MA, USA). Membranes were subsequently washed 4 times in PBS + 0.1% Tween before incubating with fluorescent secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... Protein Detection System (EMD Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 System for immunohistochemistry (Millipore).
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Cell Biology 2023Quote: ... An ECL detection system (Millipore) was used to visualize protein bands ...
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bioRxiv - Neuroscience 2021Quote: ... The RSCs from both mice were placed together and the tissue was cut into small pieces and transferred into a 1.5 ml microcentrifuge tube with 3 mg/ml isolation solution containing pronase (Sigma, Cat#P6911-1G), 1% BSA and 50 μg/ml DNaseI (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... organelle and nuclear fractions were harvested every 12 h for 2 days using the Nuclei EZ Prep nuclei isolation kit (Sigma NUC-101) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Golgi apparatus was isolated from mouse liver or cultured HCs by sequential sucrose gradients using a Golgi Isolation Kit (GL0010-1KT, Sigma-Aldrich, USA), based on the protocol we previously established (Tang and Wang ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... washed in DEPC treated 1X-PBS and then proceeded for total RNA isolation by the Trizol method as prescribed by the manufacturer’ protocol (Sigma Aldrich, India). RNA pellets so obtained were dissolved in nuclease free MQ water and quantified spectrophotometrically ...
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bioRxiv - Cell Biology 2021Quote: For islet isolation euthanized mice were perfused through the bile duct with Hank’s solution containing collagenase type XI (Sigma, St. Louis, MO). Pancreases were removed ...
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bioRxiv - Bioengineering 2021Quote: ... EVs were collected by a size-based EV isolation method [35, 36] using 50 nm membrane filter (Whatman, WHA110603) with holders (EMD Millipore, SX0002500). Holders with 50 nm membrane filters were connected to a vacuum manifold (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... sEV fractions were collected by a size-based EV isolation method using 50-nm porous membranes (Whatman, WHA110603) with holders (EMD Millipore, SX0002500) by applying a vacuum pressure ...
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bioRxiv - Plant Biology 2022Quote: ... 48 and 81) from ten time points during autumn (225-270 DOY) 2018 using Spectrum™ Plant Total RNA isolation kit (Sigma-Aldrich) according to manufacturer’s protocol (n=3 individual trees per genotype and time point) ...
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bioRxiv - Microbiology 2024Quote: ... Bronchi were cut into 5 mm strips and incubated in isolation medium with 1.4 mg/mL Pronase (Sigma-Aldrich, St. Louis, MO) and 100 μg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... To induce the auxin-degron system in DLD-1 cells, 500 µM indole-3-acetic (A10556, Fisher) and 500 µg ml−1 doxycycline (Sigma, D9891) were added to the media and cells incubated for 24 h.
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with DMEM-BSA and imaged in a Live Cell System chamber (Pathology Devices) at 37°C with 5% CO2 in the presence of 50 μg/ml PI (Sigma-Aldrich) with or without 50 µM blebbistatin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Neuroscience 2022Quote: FLAG-APP770 was expressed in BMECs using an adenoviral system and purified from cell lysates with anti-FLAG M2-agarose (Sigma-Aldrich). The lyophilized sample (30 μg of protein ...
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bioRxiv - Microbiology 2022Quote: ... Secreted CPSs from each cryptococcal strain cells were collected and purified by ultrafiltration using an Amicon® system with a membrane cutoff of 10 kDa (Millipore). The effective diameter and the polydispersity of the secreted polysaccharide preparations were measured in a 1 mg/mL solution (weight per volume ...
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bioRxiv - Microbiology 2023Quote: ... The DNA content of the cells was analyzed using the ImageStreamX MarkII Quantitative imaging analysis for flow cytometry system (Merck Millipore, Germany). About 20,000 cells were collected for each sample and the data of the single cells were analyzed with the Flowjo software.
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT host cells harboring the Flp-In™ T-REx system were maintained in culture medium (DMEM-high glucose (Sigma) medium ...