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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10-μg protein samples were separated on NuPAGE 4%-12% Bis-Tris protein gels and transferred onto Immobilon-P PVDF membranes (EMD Millipore, Billerica, MA). The membranes were incubated for 1 hour in blocking buffer (Tris-buffered saline with 0.1% Tween and 5% nonfat dry milk ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... we coated plates with 1 µg/ml of recombinant NP119 and used a goat anti-mouse IgG-HRP (ψ-chain specific) as secondary antibody (Sigma, A3673-1ML; diluted 1:1000). To measure anti-drug antibody responses directed against the AAV-delivered YTS169-IgG2a we coated plates with 1 µg/ml of YTS169-IgG2a or YTS191-IgG2a and used a goat anti-mouse IgG1-HRP (Jackson ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...
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bioRxiv - Microbiology 2023Quote: ... were coated overnight at 4°C with 20nM rAgApyrase and an unrelated protein amongst the eight candidate proteins (AGAP007393) in carbonate bicarbonate buffer pH 9.6 (Millipore-Sigma, Burlington, MA, USA, #SRE0102). Plates were washed with 0.05% v/v Tween-20 in TBS (25 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... The elutes were incubated with S-protein beads (EMD Millipore) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Protein samples were diluted in PBS pH=7.4 (Sigma-Aldrich). Neutravidin (NAv-Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: Proteins were concentrated for structural studies using ultrafiltration (Sigma-Millipore, AmiconUltra 4 mL ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:56.6 dilution and Protein-A-agarose beads (Millipore, cat# IP02 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich). Protein lysates were mixed with 5X SDS sample buffer and boiled then resolved on SDS-PAGE gels ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were incubated with protein G agarose (IP04, Millipore), Flag affinity agaros (A2220 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were reduced with 100 mM DTT (Sigma, D9163) for 35 min at 57°C and then handled according to the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were transferred to an Immobilon-Fl membrane (Millipore). The membrane was after transfer blocked using the Odyssey blocking buffer (Licor ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to Immobilon-FL PVDF Membrane (Millipore #IPFL00010) in 1 x Tris/Glycine buffer (Biorad #161-0772 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced with 10 mM dithiothreitol (Sigma Aldrich, UK) in ammonium bicarbonate for 30 min at 37°C and alkylated with 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were precleared with protein A-Sepharose beads (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... carrier protein and 0.2 mL of 100% Trichloroacetic acid (Sigma) was added to the extracted protein fraction ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore) using a semi-dry transfer apparatus at 15 V for 30 minutes (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... protein aliquots were incubated with 10μg of free avidin (Sigma) per 1mg of input protein for 1h at 4°C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich, UK) for 30 min at 37°C and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Genetics 2021Quote: ... 1:250 rat anti-myelin basin protein (MBP) (Millipore MAB386); secondary antibodies were Alexa Fluor® conjugates from Life Technologies and used at 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (Millipore, Billerica, MA) and probed with appropriate dilutions of primary antibody followed by appropriate dilutions of horseradish peroxidase-conjugated secondary antibody ...
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bioRxiv - Biophysics 2020Quote: ... Buffer proteins were purchased from Sigma-Aldrich (St. Louis, MO), and salts were purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2021Quote: ... Protein bands were detected with either anti-Flag (Sigma-Aldrich) or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Mpro proteins were concentrated to 25 mg/ml by Millipore’s ultrafiltration columns and a final concentration of 1 mM DTT was added and stored at −80°C.
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bioRxiv - Developmental Biology 2021Quote: For Hyaluronic Acid-Binding Protein (HA-BP, Sigma-Aldrich, 385911) immunofluorescence ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Protein expression was induced with 0.1% L-arabinose (Sigma-Aldrich). Expression took place for 16 h at 15°C and 220 rpm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Systems Biology 2020Quote: ... blots were stained with Ponseau S total protein stain (Sigma) prior to blocking at room temperature (5% skim milk ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were blotted on PVDF membrane (Millipore Immobilon-P, #IPVH00010) at 100 V for 1 h at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The filtrates were then concentrated in protein concentration columns (Millipore CENTRICON YM-10 Centrifugal Filter Unit 2 mL 10 kDa ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were wet-transferred to PVDF membranes (Immobilon-P, Millipore), blocked in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore, #IPF00010) for 2 h at 30 V on ice ...
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bioRxiv - Biochemistry 2021Quote: Protein was labeled with Fluorescein Isothiocyanate (FITC, Merk, Sigma-Aldrich) according to the supplier’s instructions with slight modifications ...
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bioRxiv - Molecular Biology 2021Quote: Protein mixtures were precipitated with TCA (Sigma Aldrich, Cat# T0699) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fusion proteins were captured using V5 agarose beads (Sigma # A7345) that were pre-cleared overnight with PBS + 0.1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared with 40 μl Protein A agarose beads (Millipore), and then immunoprecipitated with specific antibodies against REST/NRSF (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... the proteins were concentrated with Amicon Ultra 4 MWCO10kDa (Millipore) to approximately 3 – 4 mL ...
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bioRxiv - Molecular Biology 2019Quote: The protein was concentrated with Amicon Ultra 4 MWCO30kDa (Millipore) at 20 °C at 1650 × g ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were reduced with 2μl of 0.2M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...