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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: The purified polyP samples were measured by Malachite Green Phosphate Assay Kits (Sigma, POMG-25H). First ...
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bioRxiv - Biochemistry 2023Quote: ... their ATPase activity was measured using the Malachite Green Phosphate Assay Kit (Sigma-Aldrich MAK307). This assay measures inorganic phosphate released upon ATP hydrolysis ...
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bioRxiv - Physiology 2023Quote: Glycerol was measured in mouse plasma samples using the Glycerol Assay Kit (MAK117, Sigma-Aldrich) following the procedure recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Glucose levels were determined by using a glucose assay kit (Sigma, # GAGO20-1KT, Darmstadt, Germany). Glucose consumption was the difference in glucose concentration compared with DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... The PLA assays were performed using Duolink In Situ green kit Mouse/ Rabbit (Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... ATP measurements were carried out using an ATP bioluminescent somatic cell assay kit (FLASC, Sigma), adapting the protocol from previous studies of human/murine blastomeres and oocytes (van Blerkom et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Neuroscience 2024Quote: The ATP/ADP ratio was analyzed using an ADP/ATP Ratio Assay Kit (MAK135, Sigma), which converts ATP and ADP into their respective intermediates and measures their concentrations in a bioluminescent assay ...
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bioRxiv - Neuroscience 2024Quote: Adenosine 5’-triphosphate (ATP) levels were assessed using an ATP Bioluminescence assay Kit (Sigma/Roche). Briefly ...
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bioRxiv - Plant Biology 2024Quote: Plant total glutathione levels were calculated using a glutathione GSH/GSSG calorimetric assay kit (Sigma) in control or 5 mM H2O2 treatment conditions.
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bioRxiv - Microbiology 2024Quote: The intracellular level of glycogen was measured using the glycogen assay kit (SIGMA MAK 016) using the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: Creatine-kinase (CK) activity was determined (Creatine Kinase Activity Assay Kit, (MAPK116-1KT, Sigma-Aldrich) in serum according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Lactate dehydrogenase (LDH) release was determined using the Lactate Dehydrogenase Activity Assay Kit (MAK066; Sigma) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ATPase activities were measured using the ATPase/GTPase Activity Assay Kit (MAK-113, Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Cellular β-actin was used as a loading control and detected using a mouse anti-β-actin antibody (catalog no. A1987; Sigma-Aldrich). Horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit secondary antibodies were used to detect the primary antibodies (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: PARP10-CD protein was auto-MARylated through incubation for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: A 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... a 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... All chemicals used for sample expansion (glutaraldehyde 50% in water, sodium acrylate, N, N’-methylenbisacrylamide, acrylamide, Proteinase K) were purchased from Sigma Aldrich and used as received.
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bioRxiv - Cell Biology 2022Quote: Late L4–young adult mlt-4:RFP mutants (GUN405) were mutagenized for 4 hr at 22 °C in 0.5 mM N-nitroso-N-ethylurea (ENU, Sigma Aldrich N3385). Worms were washed in M9 buffer (22 mM KH2HPO4 ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was then treated with 1% 3-(N,N-dimethyltetradecylammonio)propanesulphonate (Sigma-Aldrich; in 100 mM citric acid, pH 2.0) at 50°C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... and pcDNA5/FRT/TO Myc SARS CoV2 N and N 2A was transfected into HEK293T cells using GeneJuice (Novagen, Darmstadt, Germany) following the manufacturer’s protocol (12 μg of DNA/sample in total) ...
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bioRxiv - Microbiology 2021Quote: ... Protein of interest was dialyzed using Slide-A-Lyzer Dialysis Cassette 10,000 MWCO (ThermoScientific, Cat. N° 66810) and concentrated using Amicon Ultra Centrifugal filters 10,000 MWCO (Millipore, Cat. N° UFC901024). Purity was verified by Coomassie staining after SDS-PAGE and concentration was measured by Bradford assay.
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bioRxiv - Biophysics 2020Quote: ... 1.4 g of the catalyst N,N,N0,N0- tetramethylethylenediamine was added before 1.4 g of ammonium persulfate (both reagents purchased from Sigma Adrich, USA), which initialized polymerization ...
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bioRxiv - Cell Biology 2021Quote: ... the carboxylic acid groups on the Dynabeads® were activated by a 30-minute incubation with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC, cat. num. 39391, Sigma) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2020Quote: Mevaldehyde was prepared using a mevaldic acid precursor (the hemi-[N,N’-dibenzylethylenediammonium] salt of mevaldic acid) purchased from Sigma Aldrich[18] ...
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bioRxiv - Bioengineering 2021Quote: ... with Irgacure 2959 (BASF, Switzerland, MW=224.3) and 0.1% N-vinyl-2-pyrrolidone (P/N V3409, Sigma-Aldrich, St. Louis, USA). The detailed protocol is described in Day et al ...
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bioRxiv - Microbiology 2022Quote: ... n-Alkane identification and quantification was performed using an external standard mixture of C21-C40 n-alkanes (PN 04071, Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The first and second extractions were combined for each sample and 250 µL of this combined solution was added to a 300 µL glass insert in a fresh glass septum-topped vial containing and the SCFAs were derivatized using 25 µL N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA; Sigma Aldrich) at 60°C for 30 minutes.
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bioRxiv - Bioengineering 2019Quote: ... Scaffolds were thoroughly washed 5 times with water and then incubated with 4mg/ml N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (Sigma-Aldrich) and 10mg/ml N-hydroxysuccinimide (NHS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... reacted with a mixture of 50 μL N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide (MTBSTFA; Sigma-Aldrich Co. LLC., St Louis, MO, U.S.A.) and 50 μL acetonitrile at 60°C for an hour.
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bioRxiv - Immunology 2020Quote: Nexterion-E microscope slides (Schott, Jena, Germany) were amino-functionalized with a 2% w/v linear copolymer of N,N′-dimethylacrylamide (Sigma-Aldrich) and aminoethyl methacrylate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were then derivatized for gas chromatography analysis using 50 µL solution of 1:1 pyridine: N-Methyl-N-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (chemicals obtained from Sigma Aldrich) and incubated at 60 °C for 30 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... Carboxyl groups on the photo-activated regions were activated with 50 µL/well of 0.05 g/mL N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 03450) and N-Hydrosuccinimide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... DiD staining solution was freshly prepared prior to every assay as a 1:200 dilution of the DiD stock solution [2 mg/mL dilution in N,N-dimethyl formamide (Sigma, #D4551)] in M9 1X buffer ...
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bioRxiv - Cancer Biology 2021Quote: For in vivo studies, AZD5153 (Chemitek, #CT-A5153), AZD5363 (Chemitek, #CT-A5363) and NHWD870 were first dissolved in N,N-dimethylacetamide (Sigma, #270555) and then mixed with 0.5% methylcellulose (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... All chemicals used for sample expansion (glutaraldehyde 50% in water, sodium acrylate, N,N’-methylenbisacrylamide, acrylamide) were purchased from Sigma Aldrich and used as received ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were derivatized for GC-MS analysis by adding 10 μL of pyridine and 50 μL of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Sigma-Aldrich) to the residue ...
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bioRxiv - Systems Biology 2023Quote: ... followed by an incubation at 40°C for 1 h and then adding 70 μl of MSTFA (N methyl-N-trimethylsilyltrifluoroacetamide; Sigma-Aldrich) followed by another incubation at 40°C for 2 h ...
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bioRxiv - Bioengineering 2023Quote: ... Then the samples were immersed in a pre-gel solution containing 30 wt % hydrogel monomers (N,N′-dimethylacrylamide; DMAA, Sigma-Aldrich) and 1 wt % Irgacure 2959 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: AD-293 cells were seeded and transfected the next day with either pcDNA5/FRT/TO-N-FLAG-hBirA*-GEMC1/MCIDAS or pcDNA5/FRT/TO-N-FLAG-hBirA* using PEI (Sigma-Aldrich) ±50 μM biotin (IBA GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... male (N = 2) and female (N = 2) naïve mice were infused with Fluorogold (Sigma, St. Louis, MO; 4% in sterile saline) in the DMS (0.2 µL) ...
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bioRxiv - Physiology 2023Quote: ... at 60°C for 60 min and derivatization with 60 µL of N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) with 1% tertbutyldimethyl-chlorosilane (TBDMCS) (both from Sigma-Aldrich). The suspension was further incubated at 60°C for 60 min ...
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bioRxiv - Cancer Biology 2024Quote: ... at a concentration of 20 mg/mL for 60 min at 37°C and 20 mL of N-tert-butyldimethylsilyl-N-Methyltrifluoracetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (#375934, Sigma-Aldrich) for 60 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... after which the rats in the ACTH group (n=50) were administered with either 3 injections of imipramine (n=10) (IMI, 15 mg/kg i.p. (Sigma-Aldrich, Denmark)) or 1 injection of saline ...
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bioRxiv - Synthetic Biology 2024Quote: ... Magnesium-Ionophor I (N,N’-Diheptyl-N,N’-dimethyl-1,4-butandiamid)(cat #63082) and α-hemolysin from Staphylococcus aureus (cat #H9395) were purchased from Sigma-Aldrich. Magnesium acetate tetrahydrate (cat #A322119 ...
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bioRxiv - Molecular Biology 2024Quote: ... N-(4-ethylphenyl)-2-[[4-ethyl-5-(3-pyridinyl)-4H-1,2,4-triazol-3-yl]thio}acetamide (ORco Receptor Agonist ORcoRAM2; OA) from Asinex Corporation and Vitas M Chemical Ltd; N,N-diethyl-3-methylbenzamide (DEET; V) from Sigma-Aldrich; and coelenterazine from Biosynth ...
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bioRxiv - Genomics 2023Quote: ... The culture medium was then aspirated and cells were lysed by adding 600ul buffer RLT from RNeasy Mini kit with 1:100 V/V β-mercaptoethanol (Sigma M3148-250ML) to the dish ...