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Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for Mouse Anti Hepatitis C Virus NS5a Antibody 1877 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with a goat anti-choline acetyltransferase antibody (ChAT; 1:1000; Millipore Cat# AB144P, RRID:AB_2079751) in Tris-Triton buffer containing 2% donkey serum and 0.2% Triton X100 ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with the primary antibodies (12h at 4 °C): anti-ChAT (1:500, goat, Sigma-Aldrich, AB144P). Then the sections were washed in PBS five times at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with rabbit anti-HA antibody (1:1000, Cat no. H6908, Sigma-Aldrich, USA). For c-Fos ...
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bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated ON at 4°C with the following primary antibodies diluted in blocking buffer: anti-FLAG (1:200, M2 clone, catalog no. F1804, Sigma-Aldrich); anti-FLAG (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at 4°C with the primary antibodies (Abs): 1:400 rabbit anti-GFAP (EMD Millipore catalog#ABS5804); 1:1000 rabbit anti-Iba1 (Wako catalog#019-19741) ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 48 hours at 4°C with the following primary antibodies: anti-GBA (1:500; human 8E4; human and rodent G4171, Sigma-Aldrich), anti-human α-synuclein (syn211 ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4°C and followed by 1-h incubation at room temperature with HRP-conjugated anti-rabbit secondary antibody (1:2000, Sigma Aldrich). Chemiluminescence was detected using ECL Western Blotting substrate (Merck ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... then blocked with 4% NHS/TBST-0.2% and incubated overnight at 4°C in primary antibody solution (1:2500 anti-tyrosine hydroxylase, clone LNC1, Millipore, Billerica, MA) in 2% NHS/TBST-0.2% ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation at 4°C in a 1:500 dilution of the anti-Calbindin-D-28K antibody (Sigma, CB-955) in blocking solution for 48 hours ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma, F3165). Each reaction was incubated with Protein-G agarose beads (50 μL ...
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bioRxiv - Physiology 2021Quote: ... Sections were then incubated for 45 minutes at 37°C with the primary antibody at 1:100 for anti-laminin (L9393, Sigma-Aldrich). Three washes were then performed with PBS ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated overnight in the same solution at 4°C with a 6000 X dilution of anti-HA-peroxidase antibodies (Sigma-Aldrich) in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared lysates (13,000 rpm, 10 min, 4°C) were incubated with 35 µl anti-FLAG antibody (Sigma-Aldrich, #F1804-1MG, RRID:AB_262044) for 30 min at 4°C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour and incubated overnight at 4°C with primary antibodies to label cells containing tyrosine hydroxylase (anti-tyrosine hydroxylase monoclonal, 1:1000 Millipore, MAB318) and mCherry (anti-dsRed polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were blocked with 5% BSA in 0.1% Triton X-100 for 2 hours and the same solution was used for overnight primary antibody dilution at 4°C with anti-FLAG M2 (Sigma, F1804) 1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were blocked with 5% BSA in 0.1% Triton X-100 for 2 hours and the same solution was used for overnight primary antibody dilution at 4°C with anti-FLAG M2 (Sigma, F1804) 1:300 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections on Visium Spatial Gene Expression Slide were fixed with methanol at −20 °C for 30 min and labelled with an anti-αSMA antibody (Cy3 conjugated, 1:2000, Sigma C6198) for 30 min at room temperature followed by DAPI nuclear counter stain ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-rabbit-HRP or anti-mouse-HRP 1:10000 (Millipore).
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bioRxiv - Pathology 2021Quote: ... mouse anti-phospho-H2AX and rabbit anti-S100A4 (Merck Millipore), rabbit anti-CD31 and rabbit anti-VEGFA (Abcam) ...
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bioRxiv - Neuroscience 2023Quote: ... and hamster anti-Zbtb7a or mouse anti-GFAP (Sigma G3893). The primary antibodies were added in 5% BSA and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... anti-USP14 (anti-mouse, Sigma, Cat#SAB1406778, 1:2500 dilution) and anti-HSC70 antibody (anti-mouse ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-p440WRN (Abgent) and mouse anti-RPA34-20 (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Cell Biology 2020Quote: Proteins tagged with HA (Mps2-3HA and 3HA-Mps2) were detected by an anti-HA mouse monoclonal antibody (1:1,000 dilution, 12CA5; Sigma). Similarly ...