Labshake search
Citations for Millipore Sigma :
3401 - 3450 of 10000+ citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... MIA PaCa-2 cells were treated with 0.1 % v/v DMSO vehicle control or with compounds at 37 °C for 4 h and then stimulated with 200 ng/mL human epidermal growth factor (hEGF, #E9644, Sigma) at 37 °C for 10 min ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... A constant amount of plasmid cDNA (15 μg) was transfected into human embryonic kidney cells 293 T (HEK-293T) using polyethylenimine (PEI; Sigma) in a 1:2 weight ratio in 10 cm plates ...
-
bioRxiv - Pathology 2023Quote: ... human RPE cells were subjected to treatment with 0.2 μg/ml doxorubicin (DOX) (Sigma-Aldrich, #D1515, St. Louis, MO, USA) for a duration of 3 hours in a culture medium containing 0.5% fetal bovine serum (FBS) ...
-
bioRxiv - Microbiology 2023Quote: ... parasites were cultured within O+ human erythrocytes at 5% haematocrit in RPMI 1640 complete medium containing L-glutamine and 25 mM HEPES (Sigma), supplemented with 0.2% sodium bicarbonate ...
-
bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
-
bioRxiv - Physiology 2023Quote: Glycerol stocks of pLKO.1-puro lentiviral plasmid vectors containing shRNA targeting human Nup93 (NM_014669) and an empty vector insert (shEmpty) were purchased from Sigma-Aldrich. Lentivirus expressing shNup93 or shEmpty were generated by PEI-mediated co-transfection of pLKO.1-puro ...
-
bioRxiv - Immunology 2022Quote: ... in mid-logarithmic phase was added at a multiplicity of infection of 2 in RPMI with 10% human serum (Sigma). Bacteria were spun down for 5 min at 800 x g and incubated for 1 h at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: The mouse E0771 (derived from a spontaneous triple negative mammary tumor that arose in a C57BL/6 mouse) and the human K562 (originally generated from a female leukemia patient) cell lines were cultured in RPMI-1640 (Sigma) supplemented with 10% FCS (GIBCO) ...
-
bioRxiv - Cancer Biology 2023Quote: The U251-MG (U251) human glioblastoma cell line was obtained from ATCC and cultured in low- glucose DMEM (Sigma, D5921) with the addition of 10% FCS ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed and incubated with 100 μl per well of samples prepared as described above and with mouse anti-human αvβ6 (Millipore, MAB2077Z) from 312.5 ng/ml to 1.22 ng/ml to produce the standard curve for 60 min at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... GFP measurements in human cell lines were taken on day 4 post-infection and every four days with Guava Easycyte HT instrument (Millipore). The fold change in GFP+ population (normalized to day 4 ...
-
bioRxiv - Microbiology 2023Quote: ... a pellet of neutrophils was resuspended in 800 μL HBSS with calcium and magnesium and 200 μL human serum (Sigma). Each 10 mL lithium-heparin coated tube of blood drawn yielded 1 mL of neutrophil-only solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... and RANTES were simultaneously quantified from the plasma collected from the BM of patients enrolled in the clinical trials using the MILLIPLEX Human Cytokine/Chemokine/Growth Factor Panel A (HCYTA-60K-PX38, Millipore) according to the manufacturer’s guidelines ...
-
bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
-
bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
-
bioRxiv - Physiology 2024Quote: ... Serum samples were stored at −80 °C until assessed by ELISA according to manufacturer’s instructions (Ultrasensitive Human Growth Hormone ELISA, 22-HGHHUU-E01, ALPCO; mGH, EZRMGH-45K and Rat/Mouse Insulin ELISA, EZRMI-13K, Millipore).
-
bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
-
bioRxiv - Immunology 2024Quote: ... An equal volume of IgM antibody at a concentration of 150-200µg/mL was mixed with equal volume of human IgG obtained from blood (Sigma-Aldrich) at a concentration of 1mg/mL (ratio ∼1:5 ...
-
bioRxiv - Immunology 2024Quote: ... Human PBMC were pre-activated with 30 ng/ml anti-CD3 (OKT3, Miltenyi) and 50 IU/ml IL-2 (Sigma) and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma ...
-
bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from human RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Sigma). Proteins were diluted in 4x Laemmli sample buffer ...
-
bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
-
bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
-
bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
-
bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
-
bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
-
bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
-
bioRxiv - Neuroscience 2024Quote: The cortex tissue samples from mice or human were lysed in PBS along with PDE inhibitor (IBMX) and PIC (SIGMA) with homogenizer followed by centrifugation at 12500 rpm for 20min at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... under air-liquid interface conditions in Greens+ keratinocyte proliferation media (Greens media supplemented with 10 ng/mL human EGF (Sigma)) ...
-
bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
-
bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
-
bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
-
bioRxiv - Physiology 2023Quote: ... 1 mg·ml−1 trypsin inhibitor and 1 mg·ml−1 bovine serum albumin (Sigma, UK) at 37°C for 45 minutes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Necrostatin-1 (Nec-1, Sigma), GSK872 (a gift from Anaxis Pty ...
-
bioRxiv - Molecular Biology 2020Quote: ... Endothelin-1 (ET-1, Millipore), isoproterenol hydrochloride (Iso ...
-
bioRxiv - Cell Biology 2020Quote: ... CASPAR-1 (Sigma, 1:500) and GAPDH (Cell Signaling ...
-
bioRxiv - Cell Biology 2022Quote: ... 1:1 Nutrient Mixture (Sigma), 1X N2 supplement (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... 1 g·L-1 metronidazole (Sigma) and 0.5 g·L-1 vancomycin (Macklin) ...
-
bioRxiv - Microbiology 2021Quote: ... 1 g·L-1 neomycin (Sigma), 1 g·L-1 metronidazole (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: ... Endothelin-1 (ET-1, Sigma) was added to the culture at a final concentration of 10 nM and the cell index was recorded in 15-second intervals for 2.5 hours and 15-minute intervals for the next 27.5 hours ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1% glutamax and 1% anti-anti) at a 1:1 ratio and neutralized using 1 M NaOH (Sigma-Aldrich). The neutralized ECM solution was mixed with 3 mg/mL rat tail collagen type I (Corning ...
-
bioRxiv - Physiology 2022Quote: ... ZO-1 (WB 1:500, IF 1:50, Millipore, AB2272), α-catenin (WB 1:500 ...
-
bioRxiv - Immunology 2021Quote: The S-variant and RBD coupled bead sets were incubated with sera from immunized mice at 4 dilutions (1:100; 1:1000; 1:10000; 1:100000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) and analyzed on a Luminex analyzer (Luminex / R&D Systems ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...