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Citations for Millipore Sigma :
3351 - 3400 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... then proteins were extracted by adding 5 volumes of protein extraction buffer containing 1X MS-SAFE protease and phosphatase inhibitor (Sigma MSSAFE) and 1mM PMSF ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The obtained protein lysates were pre-cleared by centrifugation and incubated with 100 μl (50% slurry) of protein A beads (Millipore Sigma), coated specific antibodies or corresponding species-specific IgG isotype control in 1 ml of 1 × IP buffer ...
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bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: Antigen functional activity was confirmed by assessing ACE2 binding relative to a spike protein positive control (Callaghan Innovation, New Zealand) and ovalbumin negative control protein (Sigma, #A5503). ELISA plates were coated for 1 hour by incubation at ambient temperature with 50 μL antigen (0.072 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were mixed with 200uL of pre-washed slurry of a 1:1 mix of EZview Red Protein G and EZview Red Protein A bead affinity gel slurry (Sigma-Aldrich) and rotated at 4C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted with 4 mL of elution buffer and concentrated to 1 mL in a 3K protein concentrator (Millipore Sigma). Concentrated protein was purified by SEC as described above.
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bioRxiv - Cell Biology 2024Quote: ... Pelleted debris was discarded and supernatants (solubilized mitochondrial proteins or protein complexes) were transferred to fresh tubes and coomasie brilliant blue G (CBBG, Sigma B0770) was added from a 5% stock to give a 4 g:g (digitonin:CBBG ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2021Quote: The levels of 32 different cytokines and chemokines in mouse sera were measured using a Milliplex MAP Mouse Cytokine/Chemokine premixed 32 Plex (Millipore), following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2020Quote: ... the plates were incubated with the mouse anti-HA antibody followed by goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich). The absorbance at 405 nm was read by the microplate reader (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse anti-Ankyrin-G (Neuromab N106/36; IF 1:200) Mouse anti-ß-actin (Sigma A5316; Western blot 1:5000), Rabbit anti-ß-actin (Cell Signaling Technologies #4907 ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... rabbit anti-phospho-eIF2α (1:1000, CST), mouse anti-eIF2α (1:1000, CST) or mouse anti-GAPDH (1:20000, Millipore) antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit, AB15580, Abcam, 1:250; TuJ1: mouse, MAB1195, R and D Systems, 1:100; GFAP: mouse, clone GA5, MAB3402, Millipore, 1:500 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200; Acta2, mouse, Sigma, 1:800 ...
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bioRxiv - Biochemistry 2021Quote: ... and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL; goat anti-mouse IgG, Atto647N, Sigma Aldrich, Germany) were mixed in one batch and offered in a volume of 2 µL to the beads from the opposite side ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406 ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Immunology 2021Quote: ... mouse monoclonal anti Rho-GDI (Santa-Cruz Biotechnology, 1:500) and mouse monoclonal anti-FLAG M2 Ab (Sigma, 1:1000), anti-ERK1/2 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Cell Biology 2022Quote: ... Protein complexes were identified by adding anti-mouse IgG agarose (and mouse IgG agarose as control, #A6531 and #A0919 respectively, both Sigma) for 1 hour at 4ºC in rotation ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (rat anti-BrdU, 1:100, Abcam; mouse anti-BrdU, 1:100,BD Biosciences; mouse anti-Nestin, 1:300, Millipore; rabbit anti-GFAP ...
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bioRxiv - Microbiology 2022Quote: ... the microarrays were overlaid with the VP1 proteins for 90 minutes as protein-antibody complexes that were prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: ... the microarray was overlaid with the VP1 protein for 90 minutes as a protein-antibody complex that was prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Cell Biology 2023Quote: Polyclonal antibody against the C-terminus of mouse SETX-C were generated by immunizing rabbits. Mouse cDNA encoding Setx (aa. 1900-2701) was subcloned into pET15b (Novagen) and transformed to E.coli strain BL21-CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Vimentin mouse monoclonal (1:1000, 1A4, Sigma), anti-Fibronectin rabbit polyclonal (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies to β-actin (Mouse Monoclonal, #A5441 (Sigma), 1/10000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti β-actin 1:20,000 (Sigma, A1978), rabbit anti-ubiquitin 1:1,000 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Calretinin (1:250, Cat. #: AB5054, Millipore); rabbit anti-phospho-Erk1/2 (1:250 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin (mouse, Sigma, T5168, RRID 477579). Antibodies were raised in rabbits against purified ...
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bioRxiv - Molecular Biology 2020Quote: ... Flag mouse monoclonal antibody (M2, Sigma, 1:1000), HA.11 mouse monoclonal antibody (16B12 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-insulin (1:350, Sigma, #K36AC10), mouse monoclonal anti-proinsulin (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-catenin ascites fluid (C7082, Sigma); rabbit Ab to α-catenin (ab2981 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-α tubulin (T9026, Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... or β-actin (mouse monoclonal AC15, Sigma-Aldrich). Proteins were visualized with goat anti-mouse or goat anti-rabbit IRDye-800 or - 680 secondary antibodies (LI-COR Biosciences ...